Figure 9. Asparate and pyruvate supplementation inhibits the effects of metformin on cell proliferation.

Cells were seeded and after 24 h were treated with either control, metformin (10 mM) with or without (A) aspartate (100 uM) or (B) pyruvate (1 mM). Cell proliferation was assessed after an additional 72 h by Celltiter Glo. Proliferation is displayed relative to vehicle control. ^* denotes p-value < 0.01 relative to control. ^# denotes p-value < 0.01 relative to metformin treatment.