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ΔgacI mutants do not express GlcNAc. (a) Latex agglutination reaction with GAC-specific beads. (b) GlcNAc-specific sWGA lectin staining of WT (black) and ΔgacI (white) strains. Two independent replicates containing triplicate samples were prepared, and representative data are presented (mean ± standard error of the mean [SEM]). Significant differences (P < 0.0001; denoted by ****) as determined by an unpaired Student t test are indicated.
