Table 1.
DNA and genome copy numbers measured by the three sampling techniques
| Sampling technique | Median total DNAa (min-max) (ng/cm 2) | Median bacterial DNAb (min-max) (ng/cm2) | Median fungal DNAb (min-max) (ng/cm2) | Median bacterial genome copiesb (min-max) (GCN/cm2) | Median Fungal Genome copiesb (min-max) (GCN/cm2) |
|---|---|---|---|---|---|
| Cut | 0.718 (0.638–1.764) | 0.02 (0.005–0.119) | 0.002 (0.001–0.043) | 324 (82–1773) | 1 (0.34–19) |
| Vacuum | 4.804 (2.980–12.787) | 0.597 (0.311–0.953) | 0.180 (0.103–0.341) | 15,380 (7901–24,797) | 79 (45–150) |
| Swab | 1.523 (0.978–1.898) | 0.040 (0.022–0.060) | 0.020 (0.010–0.027) | 623 (344–916) | 9 (4–12) |
Note that n = 7 for each of the sampling techniques
aMeasured using Quant-iT™ PicoGreen® dsDNA Assay Kit
bMeasured using qPCR methods