Figure 1.

Gene expression comparison of Galectins and inflammatory cytokines in lung tissue obtained from (a) ovalbumin (OVA) ‐sensitized tissue inhibitor of metalloproteinases 1 knockout (TIMP‐1 KO) mice versus wild‐type (WT) SHAM mice and (b) OVA‐sensitized TIMP‐1 KO mice versus OVA‐sensitized WT mice. Comparative real‐time quantitative PCR analysis showing relative gene expression of Galectin‐1 (Gal‐1), Gal‐3, ‐9, transforming growth factor‐β ₁ (TGF‐β ₁), TGF‐β ₃, interleukin‐1β (IL1β), interferon‐γ (IFN‐γ) and IL‐17 in lung tissue obtained from OVA‐sensitized TIMP‐1 KO mice versus WT SHAM mice (a) and OVA‐sensitized TIMP‐1 KO mice versus OVA‐sensitized WT mice (b), respectively. Data normalized to the housekeeping gene β‐actin. Gene expression was calculated using the comparative CT method. Results are expressed as the mean ± SD, n = 3 separate experiments. [Colour figure can be viewed at wileyonlinelibrary.com]