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. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: Neurotoxicology. 2017 Aug 2;64:185–194. doi: 10.1016/j.neuro.2017.07.026

Figure 2. LY294002, NU7441, KU55933, and KU60019 can reduce Mn uptake at concentrations near the IC50 for PI3K.

Figure 2

A–D) Cellular Fura-2 manganese extraction assay (CFMEA) was used to quantify 24-hour Mn uptake with increasing concentrations of NU7441, LY294002, KU55933, or KU60019. (n=3 with 6 technical replicate wells, error bars represent SD of 3 biological replicates). Red arrows denote reported IC50 for respective SMI. E) CFMEA after 24-hour Mn and/or mTOR inhibitors “mTORi” (Torin or Rapamycin).