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. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: Neurotoxicology. 2017 Aug 2;64:185–194. doi: 10.1016/j.neuro.2017.07.026

Figure 8. PI3K inhibition does not block Mn-induced p-p53 expression in Day 11 Islet 1 hiPSC-derived Neuroprogenitors.

Figure 8

A) Representative western blot of confirmed complete inhibition of Mn-induced p-p53 activity after 24 hour treatment with Mn and 1µM KU50019. B) Representative Simple Wes “lane view” image of Islet-1 neuroprogenitor lysates – cells were treated with 200uM Mn and 7µM LY294002, or 1µM KU60019 with and without 200µM Mn – probed for p-p53 (Ser15). C) Quantification of Simple Wes for p-p53/actin and normalized to respective untreated vehicle. n=3 from three separate control hiPSC cell lines. All Mn + SMI values were compared with 50µM or 100µM Mn alone by t-test. *p<0.05, **p<0.01. Error bars= SEM.