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. 2017 Dec 26;9(4):5155–5168. doi: 10.18632/oncotarget.23703

Figure 3. mRNA expression pattern of REV3L, miR-340 and RNF-130 in normal colon and colon cancer cell lines.

Figure 3

(A) mRNA expression levels of REV3L in CCD-18-Co normal coloncell line and HCT-116, DLD-1 colon cancer cells. Cell lines were analyzed for REV3L mRNA levels by qPCR. GAPDH gene was used as endogenous control. (B) miR-340 expression levels were quantified in CCD-18-Co normal colon cell line and HCT-116, DLD-1 colon cancer cell lines by qPCR. Expression of miR for each colon cancer cell line was compared with the normal cell line CCD-18-Co. U6 expression was used as endogenous control. (C) mRNA expression levels of RNF-130, the intronic region of the gene that codes for miR-340 in CCD-18-Co normal colon cell line and HCT-116, DLD-1 colon cancer cell lines. GAPDH gene was used as endogenous control. Consecutive to miR-340 expression, RNF-130 mRNA expression levels decreases in HCT-116, DLD-1 colon cancer cells compared to CCD-18-Co normal colon cell line. The data are presented as means with SDs for three independent experiments. *p<0.05; **p<0.01; ***p<0.001.