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. 2018 Feb 1;9:95. doi: 10.3389/fmicb.2018.00095

Aspergillus terreus Inhibits Growth and Induces Morphological Abnormalities in Pythium aphanidermatum and Suppresses Pythium-Induced Damping-Off of Cucumber

Boshra A Halo 1, Rashid A Al-Yahyai 1, Abdullah M Al-Sadi 1,*
PMCID: PMC5799290  PMID: 29449831

Abstract

The study investigated the efficacy of two isolates of Aspergillus terreus (65P and 9F) on the growth, morphology and pathogenicity of Pythium aphanidermatum on cucumber. In vitro tests showed that the two isolates inhibited the growth of P. aphanidermatum in culture. Investigating P. aphanidermatum hyphae close to the inhibition zone showed that the hyphae showed abnormal growth and loss of internal content. Treating P. aphanidermatum with the culture filtrate (CF) of A. terreus resulted in significant rise in cellular leakage of P. aphanidermatum mycelium. Testing glucanase enzyme activity by both A. terreus isolates showed a significant increase in glucanase activity. This suggests that the cell walls of Pythium, which consist of glucan, are affected by the glucanase enzyme produced by A. terreus. In addition, Aspergillus isolates produced siderephore, which is suggested to be involved in inhibition of Pythium growth. Also, the CFs of 65P and 9F isolates significantly reduced spore production by P. aphanidermatum compared to the control (P < 0.05). In bioassay tests, the two isolates of A. terreus increased the survival rate of cucumber seedlings from 10 to 20% in the control seedlings treated with P. aphanidermatum to 38–39% when the biocontrol agents were used. No disease symptoms were observed on cucumber seedlings only treated with the isolates 65P and 9F of A. terreus. In addition, the A. terreus isolates did not have any negative effects on the growth of cucumber seedlings. This study shows that isolates of A. terreus can help suppress Pythium-induced damping-off of cucumber, which is suggested to be through the effect of A. terreus and its glucanase enzyme on P. aphanidermatum mycelium.

Keywords: biocontrol, antagonistic activity, cucumber damping-off, oomycete, biological control

Introduction

In Oman, over 90% of greenhouses are dedicated exclusively for cucumber (Cucumis sativus). However, cucumber production suffers from Pythium-induced damping-off disease which is responsible for over 75% mortality in cucumber seedlings (Al-Kiyumi, 2006; Al-Sadi et al., 2012). Damping-off of vegetable crops is caused by several species of Pythium (Kraus and Loper, 1992; Al-Sadi et al., 2010), Rhizoctonia solani (Asaka and Shoda, 1996; Sadeghi et al., 2006), Phytophthora capsici (Sharifi-Tehrani and Omati, 1999), and Fusarium (Berg et al., 2017; Hrunyk et al., 2017; Lamprecht and Tewoldemedhin, 2017). P. aphanidermatum is the main causal agent of damping-off of cucumber in Oman and elsewhere (Al-Sadi et al., 2011, 2012; Hatami et al., 2013).

Several methods are used to manage damping-off disease, which include chemical, physical and biological methods. Chemical control of Pythium damping-off is practiced through the use of Mefenoxam, Hymexazol, Propamocarb, and other fungicides (Papavizas et al., 1978; Al-Sa’di et al., 2008, Al-Sadi et al., 2015a). Growers usually use solarization in summer to reduce Pythium propagules in soil (Deadman et al., 2007). Biological control, using microorganisms to inhibit plant pathogens, offers another alternative to chemical control. Several studies indicated the successful use of biocontrol agents to suppress Pythium damping-off of cucumber. These include the use of biocontrol agents such as Pseudomonas fluorescens, Trichoderma harzianum, and Penicillium stipitatum (Georgakopoulos et al., 2002; Al-Hinai et al., 2010; Al-Sadi et al., 2015b).

Endophytic fungi promote biotic stress tolerance, including disease stress, to host plants and they have critical roles in plant survival under stress conditions. Various endophytic fungi and bacteria, including Actinomycetes, Bacillus, Pseudomonas, Trichoderma, and Epicoccum were reported to elicit plant disease tolerance in tomato, cotton, chilli, potato and cacao (Rajendran and Samiyappan, 2008; Lahlali and Hijri, 2010; Muthukumar et al., 2010; Goudjal et al., 2014). There are several mechanisms followed by endophytic fungi and bacteria in the biocontrol of pathogens. These include the synthesis of secondary metabolites such as antibacterial, antifungal and anti-insect substances (Xiao et al., 2014; Mousa et al., 2016; Burgess et al., 2017), competition in rhizosphere (Weller, 1988; Whipps, 2001) and the induction of defense responses in plants against pathogens (Yedidia et al., 1999; Howell, 2003). Others play an important role in mineral and element solubilisation for plant absorption and nutrition (Wakelin et al., 2004; Zhang et al., 2013).

Aspergillus terreus is a common fungus in soil and plants (Tarafdar et al., 1988; Khan et al., 2010). A study showed that an antifungal compound from A. terreus effectively inhibited the phytopathogenic fungi Botrytis cinerea, Rhizoctonia solani, and Pythium ultimum (Kim et al., 1998). Another study revealed the effect of Aspergillus species bioactive metabolites on Pythium ultimum control (Abdallah et al., 2014). Moreover, applying A. terreus provided effective disease control to soil infested with P. deliense that causes damping-off disease of maize (Abdelzaher et al., 2000). Also, the combined treatment of A. terreus and Acremonium strictum led to antagonistic influence on root-knot disease of tomato caused by Meloidogyne incognita (Singh and Mathur, 2010).

To our knowledge, the efficacy of A. terreus to control P. aphanidermatum-induced damping-off of cucumber has not yet been reported, therefore, this study aims to investigate the ability of the endophytic fungus A. terreus to suppress P. aphanidermatum and Pythium-induced damping-off of cucumber. Objectives of this research work were (1) to select and identify endophytic fungi from plants in Oman which are effective in suppressing P. aphanidermatum; (2) to determine the morphological changes of P. aphanidermatum under A. terreus treatments using light microscope and scanning electron microscope; (3) to determine the effect of A. terreus culture filtrate (CF) on spore production and cellular leakage of P. aphanidermatum; and (4) to investigate the ability of A. terreus in the biocontrol of damping-off of cucumber. The selection of Rhazya stricta and Tephrosia apollinea plants for the isolation of endophytic fungi was mainly due to the intention to isolate endophytes which are present in/on native plants, and not on cultivated plants on which endophytes might be introduced from abroad. This will help come up with antagonistic fungi adapted to conditions of this part of the world.

Materials and Methods

Collection, Isolation, and Identification of Aspergillus Isolates

Fresh plants of Rhazya stricta and Tephrosia apollinea were collected from Haima and Adam, in the Sultanate of Oman in May 2016. Endophytic fungi present in the samples were isolated using a modified method of Larran et al. (2002). Briefly, root, shoot and leaves were washed under running tap water and cut into several pieces (approximately 5 mm diameter). Then, they were surface sterilized by dipping successively into 70% ethanol for 1 min, sodium hypochlorite 1% for 1.5–2 min, and finally rinsed twice in sterile distilled water. Four pieces of each sample were placed in each Petri dish containing 2.5% potato dextrose agar (PDA). Dishes were incubated in darkness at 27°C for 7 days and checked every 2 days for the emergence of endophytic fungi. Colonies growing on plates were then transferred to PDA plates. For the biocontrol study, P. aphanidermatum strain SQUCC002 was obtained from the Sultan Qaboos University culture collection.

To identity the isolated Aspergillus to the species level, total genomic DNA was extracted from freeze dried mycelium using the protocol of Lee and Taylor (1990). The internal transcribed spacer region of the ribosomal RNA (ITS), b-tubulin (TUB) and Calmodulin (CMD) regions were amplified using the primer pairs ITS1 and ITS4 (White et al., 1990), BT2A/BT2B (Koenraadt et al., 1992), and CMD5/CMD6 (Hong et al., 2005), respectively. The temperature profile for the ITS was an initial denaturation step for 10 min at 95°C, followed by 35 cycles of denaturation at 95°C for 30 s, annealing at 55°C for 30 s and extension at 72°C for 90 s and a final extension step of 72°C for 10 min. The temperature profile for TUB and CMD was an initial denaturation step for 5 min at 94°C, followed by 35 cycles of denaturation at 94°C for 45 s, annealing at 55°C for 45 s and extension at 72°C for 60 s and a final extension step of 72°C for 7 min. Purification and sequencing of PCR products were carried out at Macrogen, Korea. Sequences were aligned and improved using MEGA v.6 (Tamura et al., 2013). A maximum likelihood analysis was performed by using raxmlGUI v.1.3 (Silvestro and Michalak, 2012) to identify the species of Aspergillus using the combined alignment of ITS, TUB, and CMD regions. The optimal ML tree search was conducted with 1000 separate runs, using the default algorithm. Bootstrap 50% majority-rule consensus trees were generated and the final tree was selected among suboptimal trees from each run by comparing likelihood scores under the GTRGAMMA substitution model. Sequences generated from the analysis were deposited in GenBank under the accession numbers: ITS (65P: MG050978, 9F: MG050979), TUB (65P: MG050980, 9F: MG050981), and CMD: (65P: MG050982, 9F: MG050983).

Effect of Aspergillus terreus on Growth and Morphology of Pythium aphanidermatum

The antagonistic activity of Aspergillus terreus 65P and 9F isolates was checked against P. aphanidermatum in vitro. A 3-mm diameter disk of 2-day old P. aphanidermatum culture was placed on the edge of PDA plates. On the opposite edge, a 3-mm diameter disk of 7 day-old A. terreus culture was placed. The plates were incubated at 28°C until fungal mycelia of the control plate of P. aphanidermatum covered the agar surface. After that, the inhibition zone length (mm) was measured for all plates. The experiment was repeated twice using three replicates each time.

The changes in the hyphal morphology of P. aphanidermatum under the effect of A. terreus isolates 65P and 9F was screened using a light microscope. The morphology of 50 main hyphae and 50 hyphal tips were thoroughly examined to determine the morphological differences between P. aphanidermatum grown on PDA (Control) and P. aphanidermatum hypha close to the inhibition zone with A. terreus. Three basic morphological characteristics were screened in this study: general shape, internal content (cytoplasm), and end form of both main and hyphal tips. The experiment was repeated twice. Morphology of hyphae was also examined using a scanning electron microscope SEM (INSTUMENT JSM- 5600) at a voltage of 20 kV. A protocol described by Goldstein et al. (2003) was followed to prepare samples for the electron microscopy.

Effects of Aspergillus terreus Culture Filtrate on Electrolyte Leakage and Oospore Production of Pythium aphanidermatum

Aspergillus terreus isolates 65P and 9F were grown in potato dextrose broth (PDB) for ten days in an incubator at 28°C, and then the fungal mycelium and CF were separated by centrifugation at 10,000 g. Then CF was further filtered using Minisart filters with 0.2 μm pore size. The effect of CF of 65P and 9F CFs on P. aphanidermatum cellular leakage was studied by measuring extracellular conductivity (Lee et al., 1998; Manhas and Kaur, 2016). P. aphanidermatum was grown in PDB for 7 days at 28°C in an incubator shaker. Subsequently the fungal mycelium was obtained by centrifugation for 20 min at 10,000g. After that, the mycelium was washed in sterile distilled water and dried on sterile filter paper. Five microgram of dried mycelium was added to 10 ml of 65P and 9F CFs. The supernatants were obtained twice instantly (0 min) and after 24 h of treatment by centrifugation at 10,000g for 15 min. Conductivity meter was used to measure the extracellular conductivity for the treatments and control. The test was repeated three times.

The influence of bioactive antifungal metabolites of A. terreus strains 65P and 9F on P. aphanidermatum oospore production was studied. The CF was mixed with V8 agar at a concentration of 20%, whereas the control plates had V8 agar only. Then the plates were inoculated with 3 mm disk of P. aphanidermatum PDA plates at 28°C for three weeks. Oospores were enumerated in 30 consecutive microscopic squares at 40x magnification. There were three replicates for treatments and control.

Biochemcial Analysis of the Culture Filtrate of Aspergillus Isolates

To detect extracellular enzyme (glucanase) production by A. terreus isolates the method of Jackson et al. (2013) was followed. Briefly, glucanase substrate (4-MUB-β-D-cellobioside), 4-methylumbelliferone standard, and NaHCO3 buffer solutions were prepared. Then the CF of A. terreus and the media (PDB) were organized on a 96-well black microplate and mixed with the prepared solutions, using six replicates for each sample. Absorbance was measured at 410 nm, and the following formula was used to determine enzyme activity (Jackson et al., 2013):

Enzyme activity = (mean sample fluorescence – mean initial sample fluorescence)/((mean standard fluorescence/0.5 mol) × (mean quench control fluorescence/mean standard fluorescence) × (0.2 ml) × (time in hour)).

Analysis of Aspergillus terreus metabolites was done by growing A. terreus isolates 65P and 9F in PDB for ten days in an incubator at 28°C. Then the CF was separated from fungal mycelium spores in a centrifuge, followed by filtration through Minisart filters with 0.2 μm pore size. Liquid chromatography–mass spectrometry (LC-MS) was used for the detection of metabolites produced by A. terreus isolates depending on their masses. The CFs of A. terreus isolates 65P and 9F were concentrated by a freeze dryer machine to 25% of its total volume, then injected directly to LC-MS equipped with an electrospray ionization (ESI) source. The analysis was performed in positive polarities at ion spray voltage of +3000 V, Frag = 5.0V CF = 0.000, and DF = 0.000.

Four media were prepared to detect siderophore production by 65P and 9F strains including: King B medium consisting of glycerine 10 g/L, proteose-peptone 20 g/L, and MgSO4 1.5 g/L (de Villegas et al., 2002), glucose medium consisting of K2HPO4 0.56 g/L, Glucose 10 g/L, urea 0.85 g/L, and Glutamic acid 1 g/L (de Villegas et al., 2002), citrate medium consisting of K2HP04 6.0 g/L, KH2P04 3.0 g/L, (NH4)2S04 1.0 g/L, MgS04.7H20 0.2 g/L, and citric acid 4.0 g/L (Meyer and Abdallah, 1978) and asparagine medium consisting of Asparagine 5 g/L, MgSO4 0.1 g/L, and K2HPO4 0.5 g/L (Rachid and Ahmed, 2005). The pH for all the media was adjusted to 7.0. A 3 mm diameter mycelial disk taken from fresh potato dextrose agar plates of 65P and 9F isolates was transferred to the prepared media and incubated at 28°C on a rotary shaker (120 rpm) for 5 days. The culture broths were centrifuged at 10,000 g for 15 min and the supernatants were filtered through 0.2 μM Minisart filters. The absorbance of the supernatants was measured at 400 nm using six replicates for each sample.

The concentration was calculated according to method of Meyer and Abdallah (1978) using absorption maximum (λ = 400 nm) and molar extinction coefficient ε = 20000 (Rachid and Ahmed, 2005).

Control of Pythium-Induced Damping-Off of Cucumber by Aspergillus Isolates

The effect of 65P and 9F A. terreus isolates on Pythium damping-off of cucumber was studied using the following experimental approach. Four pots (12-cm in diameter) were used, with seven cucumber seeds sown in each pot. There were one control (irrigated with PDB) and three treatments: the first treatment was inoculated with a 57 mm plate of P. aphanidermatum, 2 cm below soil level; the second treatment was irrigated with 25 ml spore/mycelial suspension of 65P or 9F A. terreus isolates and the third treatment was inoculated with P. aphanidermatum and irrigated with 25ml spore/mycelial suspension of 65P or 9F A. terreus isolates (Al-Hinai et al., 2010). Pots were incubated in a glasshouse and the temperature was adjusted at 28°C. After 3 weeks the surviving seedlings, seedlings shoot length, seedlings fresh weight and seedlings dry weight were determined. The experiment was repeated twice.

Statistical Analysis

Data were analyzed using IBM SPSS Statistics 24.0. Treatment means ± SD were compared using independent sample t-test, One-way ANOVA and Duncan’s Multiple Range Test. Pearson Chi-Square test was used for P. aphanidermatum morphological study and Poisson test was applied for spore production count analyze.

Results

Identification of Aspergillus Isolates

Aspergillus isolate 65P was isolated from the root of R. stricta and Aspergillus isolate 9F was isolated from the root of T. apollinea. Species in Aspergillus are shown in Figure 1. The combined ITS, TUB and CMD dataset comprises 12 isolates of Aspergillus with Penicillium herquei (CBS 336.48) as the outgroup taxon. The manually adjusted dataset comprised 1664 characters including gaps (ITS: 1-565, TUB: 566-1102, CMD: 1103- 1664). A best scoring RAxML tree resulted with the value of Likelihood: –7589.972036 (Figure 1). The Aspergillus isolates in this study grouped with previously published A. terreus with high a boostrap support.

FIGURE 1.

FIGURE 1

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RaXML tree for the analysis Aspergillus spp. based on the combined ITS, TUB, and CMD gene regions. The tree is rooted with Penicillium herquei (CBS 336.48).

Effect of Aspergillus terreus on Growth and Morphology of Pythium aphanidermatum

The antagonism test showed the suppression of P. aphanidermatum growth in PDA plates under the influence of A. terreus isolates. This suppression was illustrated through the production of an inhibition zone by A. terreus. The inhibition zone produced by isolate 65P (8.66 mm) was significantly larger than the one produced by isolate 9F (Table 1).

Table 1.

Effect of Aspergillus terreus isolates 65P and 9F on the inhibition of Pythium aphanidermatum growth and the effect of their culture filtrate (CF) on extracellular conductivity (0–24 h) and spore production by P. aphanidermatum.

Inhibition zone (mm) Extracellular conductivity (mV) Spore production (no.)
Control 0 c 2.1 b 73 a
9F 5.8 b 24.6 a 17 b
65P 8.7 a 35.7 a 18 b
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Values with the same letters in the same column are not significantly different from each other (Duncan test, P > 0.05).

Microscopic examination showed that Aspergillus isolates 65P and 9F induced significant changes in the general appearance, content and ends of P. aphanidermatum hyphae (Figure 2). The effects were on the main hyphae and hypha branches close to the inhibition zone. The general appearance of hyphae became wavy (Figure 3), while for the internal content the hyphae lost most or part of its content (the cytoplasm) (Figure 3). Hyphal ends were also affected (Figure 3). Significant differences were found between the two Aspergillus isolates in their effect on general shape of main hypha and branches and also on the internal content of the main hyphae.

FIGURE 2.

FIGURE 2

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Effect of Aspergillus terreus 65P and 9F isolates on Pythium aphanidermatum morphology using light microscope. Normal general shape in control plate (A) vs. disintegrated/wavy hyphae (B); (C) the normal internal content; (D) abnormal hyphae: semi-empty pattern with content degradation under influence of 9F treatment, and abnormal hyphae: pale granular, semi empty and empty under influence of 65P (E) and 9F (F) treatments consecutively; (G) normal ends in control plate (no treatment); (H,I) wrapped up end under effect 65P treatment; (J) wrapped up end under effect 9F treatment; (K) swollen end under effect 9F treatment and (L,M) hook-like end under effect of 65P and 9F treatments, consecutively.

FIGURE 3.

FIGURE 3

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Effect of A. terreus on the hyphal morphology of P. aphanidermatum. Columns and bars represent percentage ± CV of SD. Values with the same letters are not significantly different from each other (Pearson Chi-Square: asymptotic significance, 2-sided; > 0.05).

Furthermore, the scanning electron microscope showed that Aspergillus isolates 65P and 9F caused considerable changes in P. aphanidermatum hyphal morphology (Figure 4). Most of the observed hyphal patterns were wrinkled in both treatments compared to control which had normal hyphae.

FIGURE 4.

FIGURE 4

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Effect of A. terreus on the P. aphanidermatum hyphae morphology using SEM. (A,B) Abnormal hyphae: wrinkled or shrunken patterns under effect of 9F treatment (C,D) abnormal hyphae: wrinkled or shrunken patterns with under effect of 65P treatment, (E,F) normal patterns of hyphae in the control.

Effects of Aspergillus terreus Culture Filtrate on Electrolyte Leakage and Oospore Production of Pythium aphanidermatum

Treating P. aphanidermatum with the CF of Aspergillus isolates 65P and 9F resulted in significantly higher extracellular conductivity values after 24 h in comparison with the control, with the values been 35.7 mV for 65P, 24.6 mV for 9F and 2.1 mV for the control (Table 1). This gives indication that the mycelium of P. aphanidermatum leaked electrolytes as a result of possible enzymes or metabolites produced by A. terreus.

The CFs of 65P and 9F isolates of A. terreus significantly reduced spore production by P. aphanidermatum compared to the control (P < 0.05) (Table 1). Oospores which were produced in control (73) were higher than the spores in 65P treatment (18) and 9F treatment (17).

Biochemical Analysis of the Culture Filtrate of Aspergillus Isolates

The concentration of glucanase in the CFs of 65P (3.99) and 9F (4.73) were significantly higher than the media control which was (0.47). Enzyme activity is expressed in nmoles substrate consumed h-1 ml of CF or media-1). This confrms that A. terreus isolaetes produce glucanse enzyme and its activity increases within minutes of application.

The spectra of isolate 65P CF showed a set of peaks in the positive and negative ion ESI spectra. The positive spectra showed distributions of peaks ranging between 146.8000 and 909.6000 m/z (Supplementary Figure S1). However, peaks in the negative ion mode spectra were in the range of 148.8000 to 2522.7000 m/z. Peaks in the positive and negative ion mode spectra for isolate 9F were in the ranges of 132.0000 to 928.5000 m/z and 149.0000 to 2560.3000 m/z, respectively (Supplementary Figure S2).

Siderophore production by 65P and 9F isolates in four media is displayed in Table 2. It indicates siderophore secretion by both isolates of A. terreus at varying values depending on the medium. The best medium that contains the highest amount of siderphore was King B medium for 65P isolate (93.63 μM) and 9F isolate (55.88 μM), followed by Glucose medium (63.78 μM for 65P isolate and 15.91 μM for 9F isolate). However, Citrate and Asparagine media had low amounts of siderophore compared to King B and Glucose media.

Table 2.

Effect of medium content on siderophores concentration (μM) produced by 65P and 9F isolates.

Medium King B medium Glucose medium Citrate medium Asparagine medium
65P 93.63a ± 3.74 63.78b ± 3.23 7.18e ± 0.11 6.96e ± 0.32
9F 55.88c ± 1.29 15.91d ± 0.52 6.99e ± 0.11 7.20e ± 0.1
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Values (concentration in μM ± SD) with the same letters are not significantly different from each other (Duncan test, P > 0.05).

Control of Pythium-Induced Damping-Off of Cucumber by Aspergillus Isolates

Inoculation of cucumber seedlings with P. aphanidermatum resulted in the development of damping-off symptoms, with only 10–20% of the seedlings survived. The rate of seedling survival increased to 39.3% in seedlings treated by 65P and 37.5% in seedlings treated by 9F (Figure 5).

FIGURE 5.

FIGURE 5

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The biocontrol efficacy of endophytic fungi treatments against P. aphanidermatum damping-off. (A) 65P treatment (B) 9F treatment. Columns and bars represent percentage ±95% confidence limit. Values with the same letters are not significantly different from each other (Duncan test, P > 0.05).

No significant effect was found for the 65P isolate on the shoot length of cucumber seedlings, while the 9F isolate significantly increased the shoot length of cucumber seedlings (Figure 6). No effect was found for Aspergillus isolates on the shoot fresh and dry weight (Figure 7).

FIGURE 6.

FIGURE 6

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Effect of inoculation with 65P (A) and 9F (B) fungi on cucumber seedlings shoot length. Error bars represent 95% confidence limit of a means. Values with the same letters are not significantly different from each other (ANOVA Test, P > 0.05).

FIGURE 7.

FIGURE 7

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Effect of inoculation with 65P (A) and 9F (B) fungi on cucumber seedlings shoot weight. Error bars represent 95% confidence limit of a means. The same letters are not significantly different from each other (ANOVA Test, P > 0.05).

Discussion

The current study identified Aspergillus terreus as an entophytic fungus in R. stricta and T. apollinea. A. terreus was not isolated previously from R. stricta and T. apollinea. A. terreus was identified to the species level based on sequences of the ITS, TUB, and CALMODULIN genes, which were efficient in discriminating this species from other Aspergillus species (Peterson, 2008; Arabatzis and Velegraki, 2013; Samson et al., 2014).

Aspergillus terreus 65P and 9F isolates inhibited the growth of P. aphanidermatum in vitro. A study carried out by Kumar et al. (2000) showed the production of an inhibition zone by A. terreus against Neurospora crassa. Moreover, a study by Wang et al. (2011) revealed that new compounds from A. terreus could produce inhibition zone against Pseudomonas aeruginosa and Enterobacter aerogenes growth. It is likely that an inhibition zone was produced because A. terreus secrets metabolites that interfere with the growth of P. aphanidermatum. Ferrón et al. (2005) and Wang et al. (2011) reported the production of terremides A and B and lovastatin by A. terreus that were found responsible for the production of inhibition zones. In our study, the use of CF from A. terreus isolates resulted in electrolyte leakage in P. aphanidermatum mycelium and also interfered with oospore production in P. aphanidermatum. Extracellular conductivity of Streptomyces hydrogenans supernatant that was treated with Alternaria brassicicola mycelium was increased with the progress of time compared to 0 min (Manhas and Kaur, 2016). P. aphanidermatum cell wall polysaccharides consist of 18% of cellulose and 82% of (1 → 3), (1 → 6)-β-D-glucans (Blaschek et al., 1992). Our study showed that A. terreus 65P and 9F isolates have the ability to produce glucanase enzyme. A previous study by Gao et al. (2008) proved the production of extracellular enzymes by thermoacidophilic fungal A. terreus M11. Also, the results of Djonović et al. (2006) demonstrated the involvement of β-1,6-glucanase in mycoparasitism and its relevance in the biocontrol activity of Trichoderma virens against plant pathogen Pythium ultimum. Furthermore, T. harzianum produced 1,3-β-glucanase and cellulase which led to control Pythium damping-off of cucumber seedlings (Thrane et al., 1997). In addition, El-Tarabily et al. (2009) showed that the glucanase-producing actinomycetes could replace the use of metalaxyl in the control of Pythium aphanidermatum diseases. Many other fungi have the ability to produce extracellular enzymes such as Phoma medicaginis and Penicillium citrinum (Khan et al., 2016) and Talaromyces emersonii (McHale and Coughlan, 1981). The production of extracellular enzymes by Talaromyces flavus effectively contributed to control of Sclerotium rolfsii and Verticillium dahliae (Madi et al., 1997). The electrolyte leakage, which was observed in our study, appears to be because of the glucanase enzyme production by A. terreus isolates, which appears to play a role in the biological control against P. aphanidermatum through the degradation of Pythium cell wall and release of cell components.

Aspergillus terreus has been shown to produce several metabolites (Nakagawa et al., 1982; Hendrickson et al., 1999; Gao et al., 2008; Goutam et al., 2017; Saha et al., 2017). In our study, by comparing the current molecular weight results of 65P (389.1 m/z) and 9F (390.7 m/z) with the findings from previous studies, both isolates seem to produce mevastatin (390.513 g/mol). Mevastatin production by A. terreus was reported by previous studies (Manzoni et al., 1998). It has also been reported to be produced by other fungi (Brown et al., 1976; Reino et al., 2008). Mevastatin has been reported to have biological inhibitory activities (Kumar et al., 2000). Thus, it is possible that the production of this compound and others could explain the suppression role of A. terreus isolates against P. aphanidermatum.

Our study showed that A. terreus isolates induced morphological changes in the mycelium of P. aphanidermatum. The general shape of Pythium mycelium changed from straight normal pattern to abnormal patterns as wavy, and the internal content became granular, disintegrated and lost. A previous study by Chet et al. (1981) showed that Trichoderma hamatum induced morphological changes in Pythium spp and Rhizoctonia solani, where the mycelium had bulbular or hook-like structures that contained granular cytoplasm. Another study by Paulitz et al. (2000) showed that Pseudomonas aureofaciens resulted in abnormalities in hyphal morphology of Pythium ultimum. Prapagdee et al. (2008) showed that Streptomyces hygroscopicus produced extracellular antifungal metabolites such as chitinase and β-1,3-glucanase that affected the growth and morphology of Colletotrichum gloeosporioides and Sclerotium rolfsii phytopathogenic fungi. Several metabolites such as enzymes, antibiotics and organic acids are secreted by A. terreus (Calton et al., 1978; Nakagawa et al., 1982; Hendrickson et al., 1999; Gao et al., 2008; Goutam et al., 2017; Saha et al., 2017; Sreedevi et al., 2017). In our study, it is very likely that the production of glucanase enzyme contributed to abnormalities in Pythium mycelia.

Our scanning electron microscope results showed that P. aphanidermatum hyphae became wrinkled or shrunken as well as smaller in size; however, in control the hyphae had normal cell wall morphology with a smooth surface and full content. These results indicate that P. aphanidermatum cytoplasm in the treated petri dishes plates were degraded by the effect of A. terreus isolates and their enzyme, glucanase. This shrunken morphology was observed in Fusarium oxysporum mycelia which were treated with Streptomyces cinereus (Gangwar et al., 2015).

Spore production by P. aphanidermatum has been inhibited by the CF of 65P and 9F isolates. A previous study by El-Tarabily (2006) showed that P. aphanidermatum oospores were parasitized by Actinoplanes philippinensis and Micromonospora chalcea and as a result had disorganized cytoplasm. Also, Manhas and Kaur (2016) study demonstrated suppression of germination as well as loss of pigmentation and shrinkage of Alternaria brassicicola spores due to treatment with the CF of Streptomyces hydrogenans. Only Actinoplanes campanulatus was capable of affecting P. aphanidermatum oospores in El-Tarabily et al. (2009) study.

Our results proved that both A. terreus isolates can produce siderophore, with some differences between the isolates in their ability to produce siderophore. King B was the best medium for obtaining the highest value of siderophore. Similarly Rachid and Ahmed (2005) found that king B medium is better than several other media. Many studies indicated siderophore production by several fungi such as Aspergillus terreus (Waqas et al., 2015), Aspergillus fumigatus (Haas, 2014), and Chaetostylum fresenii (Thieken and Winkelmann, 1992).

Siderophore production is an effective mechanism of biological control of multiple diseases caused bacterial and fungal agents. Siderophore production by Rhodotorula glutinis has been reported to suppress blue rot caused by Penicillium expansum in harvested apples (Calvente et al., 1999). Moreover, Bacillus subtilis inhibits Fusarium-wilt of tomatoes disease and secrets siderophore. Furthermore, the inhibition role of Pseudomonas fluorescens against several Pythium species is due to siderophore production (Weller and Cook, 1986).

The bioassay test showed a significant effect of A. terreus on the survival of cucumber seedlings inoculated by P. aphanidermatum. The A. terreus isolates did not have any negative effects on the shoot length, dry weight, or fresh weight. Various studies were carried out to characterize the biocontrol efficacy against P. aphanidermatum damping-off by multiple microorganisms such as Trichoderma harzianum (Sivan et al., 1984), Pseudomonas chlororaphis, and Bacillus subtilis (Nakkeeran et al., 2006) and endophytic actinomycetes (El-Tarabily et al., 2009). Some studies used several biocontrol species, which led to greater disease suppression (Szczech and Shoda, 2004; Liu et al., 2017). Other studies used seed treatment with biocontrol agents which proved its effectiveness in damping-off control (Lifshitz et al., 1986; Callan et al., 1990).

Our study demonstrates for the first time that A. terreus can be effectively used to manage Pythium-induced damping-off of cucumber. A. terreus was found to interfere with the growth and spore production of P. aphanidermatum and induce morphological changes in its mycelium. In addition, A. terreus produces glucanase enzyme and mevastatin, the activities of which are suggested to play a role in the antagonism against P. aphanidermatum. In addition to the active role of enzymes in biological control, several other secondary metabolites contribute effectively to the control of plant pathogens such as siderophore (Schwyn and Neilands, 1987; Naureen et al., 2017) and Hydrogen cyanide (HCN) (Ramette et al., 2003). Baakza et al. (2004) indicated production of siderophore by Aspergillus species including A. terreus. It is therefore important to conduct a separate study to look into all the possible mechanisms by which A. terreus inhibits or interfere with P. aphanidermatum. Since A. terreus is known to have some side effects on human health, future studies should take into account investigating the side effects, if any, of A. terreus when used as a biocontrol agent. These include effects of plants as well as safety during applications. The effects of A. terreus on other biocontrol agents in soil should also be investigated.

Author Contributions

BH, RA-Y, and AA-S designed the experiment. BH carried out the experiments. BH and AA-S analyzed the data. BH, RA-Y, and AA-S wrote the manuscript. All authors approved the manuscript.

Conflict of Interest Statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Acknowledgments

The authors would like to thank Dr. Sajeewa for his help in phylogenetic analysis.

Footnotes

Funding. Authors would like to acknowledge financial support from Sultan Qaboos University and Oman Animal and Plant Genetic Resources Center through the projects EG/AGR/CROP/16/01 and IG/AGR/CROP/16/03.

Supplementary Material

The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fmicb.2018.00095/full#supplementary-material

FIGURE S1

Mass spectrum (m/z) of Aspergillus terreus 65P isolate culture filtrate (CF). (A–C) Shows positive ESI scan (at 0.146 min and 100–3000 m/z), (at 156 min and 100:1075 m/z), (at 156 min and 260–920 m/z), respectively, while (D–F) show negative ESI scan (at 0.146 min and 100:3000 m/z), (at 0.146 min and 100:1250 m/z), (at 0.146 min and 1050:3000 m/z), respectively.

Click here for additional data file. (411.9KB, JPEG)
Click here for additional data file. (411.9KB, JPEG)
FIGURE S2

Mass spectrum (m/z) of A. terreus 9F isolate CF. (A–C) Shows positive ESI scan (at 0.146 min and 100–3000 m/z), (at 136 min and 100:1125 m/z), (at 136 min and 280–1140 m/z), respectively, while (D–F) show negative ESI scan (at 0.146 min and 100:3000 m/z), (at 0.146 min and 100:1300 m/z), (at 0.146 min and 500:3000 m/z), respectively.

Click here for additional data file. (374KB, JPEG)
Click here for additional data file. (374KB, JPEG)

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Associated Data

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Supplementary Materials

FIGURE S1

Mass spectrum (m/z) of Aspergillus terreus 65P isolate culture filtrate (CF). (A–C) Shows positive ESI scan (at 0.146 min and 100–3000 m/z), (at 156 min and 100:1075 m/z), (at 156 min and 260–920 m/z), respectively, while (D–F) show negative ESI scan (at 0.146 min and 100:3000 m/z), (at 0.146 min and 100:1250 m/z), (at 0.146 min and 1050:3000 m/z), respectively.

Click here for additional data file. (411.9KB, JPEG)
Click here for additional data file. (411.9KB, JPEG)
FIGURE S2

Mass spectrum (m/z) of A. terreus 9F isolate CF. (A–C) Shows positive ESI scan (at 0.146 min and 100–3000 m/z), (at 136 min and 100:1125 m/z), (at 136 min and 280–1140 m/z), respectively, while (D–F) show negative ESI scan (at 0.146 min and 100:3000 m/z), (at 0.146 min and 100:1300 m/z), (at 0.146 min and 500:3000 m/z), respectively.

Click here for additional data file. (374KB, JPEG)
Click here for additional data file. (374KB, JPEG)

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