Table 1.

Cryopreservation Parameters and Suggested Methods: A quick summary of the parameters which had the best outcome for the islets during cryopreservation. Assessment methods include islet viability, glucose sensitivity, and GSIS values after thawing.

Parameter	Method	Reference Number
Cryoprotectant	EPA+DHA+Metformin	40
Cooling Rate	Rapid (50–70°C/min)	27–29
Thawing Rate	Rapid (150–200°C/min)	32
Oxygen Environment	50% during thawing	54
3D Structure	Freeze as individual cells, re-aggregate into spheroids after thaw	51
Encapsulation	1.75% Alginate encapsulation prior to cryopreservation	Unpublished data (Lakey J, et al.)