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. 2018 Feb 6;7:e32637. doi: 10.7554/eLife.32637

Figure 4. Transient knockdown of hcrt increases sleep in four dpf cavefish.

(A) Morpholino knock-down of hcrt does not alter total sleep in surface fish, p=0.640, n = 34, while in cavefish total sleep time was significantly increased with hcrt knock-down, p=0.0002, n = 40; 2-way ANOVA, F(1,133)=45.82, p<0.0001 (B) Waking activity was not significantly altered in either surface fish (p=0.343, n = 34) or Pachón cavefish (p=0.084, n = 40; 2-way ANOVA, F(1, 133)=5.807). (C) Total number of sleep bouts in surface fish was not significantly different from injected controls, p=0.459, n = 34. While in Pachón cavefish, total sleep bouts over 24 hr was significantly increased in hcrt MO fish compared to control fish (p=0.0004, n = 40; 2-way ANOVA, F(1,133)=8.295, p=0.004). (D) Average sleep bout duration was not different between controls and hcrt MO injected surface fish (p=0.081, n = 34). There was a significant increase in average bout duration In Pachón hcrt MO injected fish compared to their respective controls (p=0.004, n = 40; 2-way ANOVA, F(1,133)=13.61, p=0.0003).

Figure 4—source data 1. Transient knockdown of hcrt increases sleep in 4 days post fertilization cavefish.
This source file includes analysis comparing the effects of hcrt morpholino treatment on sleep duration (Figure 3A) waking activity (Figure 3B), total number of sleep bouts (Figure 3C), and average sleep bout duration (Figure 3D).
elife-32637-fig4-data1.xlsx (13.4KB, xlsx)
DOI: 10.7554/eLife.32637.017

Figure 4.

Figure 4—figure supplement 1. Morpholino injections do not affect survival in development.

Figure 4—figure supplement 1.

(A) No significant difference in surface fish survival from embryo injection to 96 hr post fertilization (Kruskall-Wallis, p=0.719, Dunn’s post test, Wild Type vs. Scramble MO, p=0.862, Scramble MO vs. hcrt MO, p=0.701). (B) Pachón fish survival from embryo injection compared to controls up to 96 hr post fertilization (Kruskall-Wallis, p=0.429, Dunn’s post test, Wild Type vs. Scramble MO, p=0.299, Scramble MO vs. hcrt MO, p=0.206).
Figure 4—figure supplement 1—source data 1. The effect of morpholino injection on survival in surface fish and cavefish.
This source file includes data analysis quantifying the effects of morpholino injection on survival in surface fish (Figure 4—figure supplement 1A), and Pachón cavefish; Figure 4—figure supplement 1B over the first 96 hr post fertilization.
elife-32637-fig4-figsupp1-data1.xlsx (10.2KB, xlsx)
DOI: 10.7554/eLife.32637.019