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. 2018 Jan 8;9(7):7541–7556. doi: 10.18632/oncotarget.24045

Figure 4. Combination of PENAO with temsirolimus increases mitochondrial dysfunction and enhances apoptosis.

Figure 4

(A) Flowcytometric analysis of HSJD-DIPG007 cells for production of mitochondrial ROS. DIPG cells were treated with 0.25 µM of PENAO, 2.5 µM temsirolimus, combined agents for 18 h and subsequently stained with MitosoxRed and analysed with FACS Canto B. Data represent average and SD of 3 determinations, PENAO vs Combination p < 0.001; temsirolimus vs Combination p < 0.001; (B) Flow cytometric analysis of HSJD-DIPG007 cells for mitochondrial depolarisation. DIPG cells were treated with 0.25 µM of PENAO, 2.5 µM temsirolimus, combined agents for 18 h and subsequently stained with JC1 and analysed with FACS Canto B. Data represent average and SD of 3 determinations; PENAO vs combination p < 0.05; temsirolimus vs combination p < 0.01; (C) Flow cytometric analysis of HSJD-DIPG007 cells for apoptotic cell death. DIPG cells were treated with 5 µM of PENAO, 10 µM temsirolimus, combined agents for 48 h and subsequently stained with AnnexinV-FITC and 7AAD and analysed with FACS Canto B. Data represent averages and SD of 3 determinations. PENAO vs combination p < 0.001; temsirolimus vs combination p < 0.001.