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. 2017 Oct 23;37(6):697–709. doi: 10.1038/onc.2017.358

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Copyright © 2018 The Author(s)

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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Localization of FAM49B in the mitochondria. (a) Cells co-stained with Mitotracker (red) and anti-FAM49B antibody (green) was detected by immunofluorescence. Tissue sections were analyzed with a laser scanning confocal microscope (Zeiss LSM5 Pascal, Jena, Germany) using its multichannel acquisition mode to avoid fluorescence cross talk. Images were acquired with a × 100 oil-immersion objective, scale bar 10 μm. The pixels indicating co-localization are shown in yellow. (b) FAM49B expression in the cytosolic (Cyto) and mitochondria-enriched (Mito) fractions of HPDE, CFPAC1 and T3M4 cells was measured by western blot analysis. The mitochondrial marker ATP5A was used as a control of the quality of the mitochondrial fractions. GAPDH served as a cytosolic marker.