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. 2018 Jan 4;7:e33125. doi: 10.7554/eLife.33125
Criterion How to obtain it Example value
Exposure time Determined at the acquisition step 5 ms
Signal-to-background ratio Mean peak value of detected particle divided by mean background value 5
Detection algorithm used MTT (version xxx)
Tracking algorithm used MTT (version xxx)
Number of particles per frame Provided by Spot-On Mean: 0.76
Number of detections Provided by Spot-On 360000
Number of trajectories of length >3 Provided by Spot-On 10000
Mean trajectory length Provided by Spot-On 4.5 frames
Localization error Provided by Spot-On 30 nm