Criterion | How to obtain it | Example value |
---|---|---|
Exposure time | Determined at the acquisition step | 5 ms |
Signal-to-background ratio | Mean peak value of detected particle divided by mean background value | 5 |
Detection algorithm used | MTT (version xxx) | |
Tracking algorithm used | MTT (version xxx) | |
Number of particles per frame | Provided by Spot-On | Mean: 0.76 |
Number of detections | Provided by Spot-On | 360000 |
Number of trajectories of length >3 | Provided by Spot-On | 10000 |
Mean trajectory length | Provided by Spot-On | 4.5 frames |
Localization error | Provided by Spot-On | 30 nm |