Figure 1.

Expression profile of SiHsTRPA mRNA and the protein expression in HEK293 cells. A, Detection of SiHsTRPA mRNA and S. invicta 18S rRNA in the abdomen, antennae, head, legs, and thorax by RT-PCR. The position of 100–300-bp DNA molecular weight marker (MW) is shown at the left. B, Relative expression levels of SiHsTRPA mRNA in the abdomen, antennae, head, legs, and thorax measured by qRT-PCR. S. invicta 18S rRNA was used as the internal standard, and the level in the abdomen was set as 1. C, Proteins expressed in HEK293 cells transfected with empty vector (Mock) and SiHsTRPA-expressing construct were analyzed by Western blot. The size (in kilodaltons) of protein molecular weight marker (MW) is at the left. D, Localizations of plasma membrane-bound FITC-WGA and SiHsTRPA tagged with V5-epitope in the transfected HEK293 cells by immunofluorescence. The merged image is also shown.