Figure 2.

DNA methylation-specific gene expression in the midgut and fat body of Bt-infected resistant and susceptible G. mellonella larvae. The expression of the DNA methyltransferase 1-associated protein 1 and DNA cytosine 5-methyltransferase genes were measured in the midgut and fat body of resistant and susceptible G. mellonella larvae infected with Bt. The values were normalized to the midgut and fat body expression profiles of the corresponding uninfected resistant and uninfected susceptible larvae. The 18S rRNA housekeeping gene was used for internal data normalization. Data are means of 3 independent experiments with standard deviations (*p < 0.05, ** p < 0.005, *** p < 0.0005- fold expression in the fat body and midgut of infected susceptible line and infected resistant line were compared with fold expression of uninfected susceptible line and uninfected resistant line for the same organ respectively).