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. 2017 Dec 24;9(5):5588–5599. doi: 10.18632/oncotarget.23652

Figure 2. Alteration of MMP2 and A2M expression during aging and CR.

Figure 2

(A) Gene expression on MMP2 was detected by qPCR. The mRNA levels were normalized to the Gapdh level. #P < 0.05 vs young. *P < 0.05 vs old. (B) Western blotting was conducted to detect protein expression of MMP2. β-actin was used as loading control. The blots were quantified by densitometry (n = 8). Data are expressed as the mean ± SEM. #P < 0.05 vs young. (C) Gene expression of A2m is detected by qPCR. mRNA levels were normalized to the Gapdh level. #P < 0.05 vs young. *P < 0.05 vs old. (D) Protein level of A2M is identified by Western blotting. The blots were quantified by densitometry (n = 8). Data are expressed as the mean ± SEM. #P < 0.05 vs young. *P < 0.05 vs old.