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. 2018 Feb 11;20(3):233–243. doi: 10.1016/j.neo.2018.01.006

Figure 5.

Figure 5

Loss of Src myristoylation inhibits paracrine FGF10-induced tumorigenesis. (A) 293 T cells were transduced with control vector, 0.1, 1, and 10 μL of Src(WT) or Src(G2A) lentivirus. The transduced cells were harvested, and protein lysates were analyzed for the expression levels of Src, phospho-Src and γ-tubulin. (B-C) Phase and RFP fluorescence images (B) and weight (C) of the regenerated prostate tissues derived from PrECs-Src(WT) + GFP-UGSM, PrECs-Src(G2A) + GFP-UGSM, PrECs-Src(WT) + FGF10-UGSM, and PrECs-Src(G2A) + FGF10-UGSM groups. Scale bar, 0.5 mm. Values are mean ± SD. *: p<0.05. (D) The regenerated tissues derived from PrECs-Src(WT) + GFP-UGSM, PrECs-Src(G2A) + GFP-UGSM, PrECs-Src(WT) + FGF10-UGSM, and PrECs-Src(G2A) + FGF10-UGSM groups were analyzed for H&E staining, RFP signal, and IHC staining of Src, CK5 (red)/CK8 (green)/DAPI (blue), and E-Cadherin (red)/vimentin (green)/DAPI (blue). Scale bar, 100 μm.