Linear relationship between anxiety-like behavior, exploratory locomotion on the EPM, and nicotine responses. In Experiment 1, (A) adult males that exhibited greater preference for 100 µg/ml nicotine spent less time on the open arms of the EPM. (B) Males and (C) females that exhibited greater nicotine-induced (0.5 mg/kg, i.p.) locomotor depression during late adolescence entered the closed arms of the EPM more frequently during adulthood. In Experiment 2, (D) preference for 100 µg/ml nicotine was not associated with time spent on the open arms of the EPM. Furthermore, nicotine-induced locomotor depression during adulthood was not associated with closed arm entries on the EPM for (E) males or (F) females. Shaded regions signify 95% confidence intervals.