Abstract
Purpose: Tumour cells produce energy through glycolysis and lactate dehydrogenase (LDH) is a key part of glycolysis. Elevation of serum LDH may indicate poor prognosis in primary duodenum adenocarcinoma. We aim to explore the prognostic significance of LDH in this disease.
Methods and materials: Two hundred forty-four patients diagnosed with primary duodenum adenocarcinoma who were treated at the Sun Yat-sen Cancer Center from February 1996 to January 2016 were retrospectively analysed. We collected routine clinical data, including baseline LDH. Patients were classified into a normal LDH group (≤ 245U/L) and higher LDH group (>245U/L). Correlations of the LDH level and other clinicopathological characteristics were explored using the Chi-square test. Prognostic factors for overall survival were identified using univariate and multivariate analyses.
Results: Two hundred seven patients (84.9%) had normal LDH levels, while 37 patients (15.1%) had abnormally high LDH levels. Higher LDH levels were significantly associated with more distant metastasis, node metastasis, poor differentiation and TNM stage Ⅲ-Ⅳ (P<0.05). Consistently, patients with node metastasis, poor differentiation and TNM stageⅢ-Ⅳ had a significantly higher median LDH level (P<0.05). The median survival of patients in the higher LDH group was significantly shorter than that of the patients in the normal LDH group (16.3 m vs. 42.5 m, P=0.02). Using multivariate analysis, LDH, age and radical surgery were independent prognostic factors associated with overall survival(OS) (HR=1.571, P=0.036 for LDH; HR=1.514, P=0.013 for age; HR=0.248, P<0.0001 for radical surgery, respectively).
Conclusions: For the first time, our research suggests that baseline serum LDH is an independent prognostic factor in primary duodenum adenocarcinoma patients and elevated baseline serum LDH indicates a poor prognosis.
Keywords: lactate dehydrogenase, primary duodenum adenocarcinoma, prognosis, serum biomarker
Background
Primary duodenum adenocarcinoma is a rare tumour with a very low estimated incidence rate (<0.5/100,000) 1-2. It makes up approximately half of small bowel cancers and approximately 1% of all gastrointestinal cancers 3. Due to a lack of specific duodenal adenocarcinoma symptoms, a portion of patients are diagnosed at the metastatic stage 4. The median overall survival(OS) of metastatic duodenum adenocarcinoma, ranging from 2 to 8 months, is much worse than advanced colorectal cancer 4. Various studies have attempted to evaluate different prognostic factors for primary duodenum adenocarcinoma, and some studies have identified the T stage, nodal metastasis, pathologic grade, lymphovascular invasion, age, weight loss and perineural invasion as prognostic factors 5-12. Due to the rare incidence, the number of patients in previous studies was relatively small, with a range from 17 to 178 patients 5-12. However, none of these studies identified any prognostic serum biomarker.
The metabolism of tumour cells has its own characteristics. To coincide with the rapid development of the tumour, tumour cells produce energy through glycolysis. We call this the Warburg effect. This alternation of cancer cell metabolism is believed to be a main change in neoplastic transformation 13. The hypoxic microenvironment is another feature of malignant tumours. In conditions of hypoxia, lactate dehydrogenase (LDH) can convert pyruvate to lactate to support tumour cells. Hypoxia inducible factor 1α (HIF-1α) can transcriptionally upregulate LDH in tumour cells to ensure anaerobic glycolysis and produce enough lactate 14. High levels of lactate can in turn promote higher expression of HIF-1α. Thus, HIF-1α and LDH interact and strengthen each other 14. Furthermore, higher LDH is significantly correlated with increased vascular density and decreased infiltration of lymphocytes within the tumours. Therefore, the increased LDH level reflects these alterations and suggests more severe tumour burden, tumour angiogenesis and tumour progression conditions, all of which lead to the poor prognosis of malignant tumours 15.
Elevated serum LDH has been confirmed as a poor prognostic factor in many types of malignancies, such as renal cell cancer, nasopharyngeal carcinoma, pancreatic cancer, lymphoma, breast cancer, prostate cancer, colorectal cancer, lung cancer and oesophageal squamous cell carcinoma 16-24. Some researchers even incorporated LDH into the prognostic scores 19, 24. The serum LDH level is routinely tested in daily clinical practice, which is economical and convenient to perform. It is worthwhile for us to explore the prognostic significance of the serum LDH level in patients with primary duodenum adenocarcinoma.
For the first time, this research investigates the prognostic significance of serum LDH level in a large cohort of 244 patients with primary duodenum adenocarcinoma. The relationship between LDH and other clinical factors was also explored in the current study.
Methods
Patient selection and evaluation
We studied electronic medical records of primary duodenum adenocarcinoma patients hospitalized in Sun Yat-sen Cancer Center from February 1996 to January 2016. Written informed consent was provided by all patients included in our study. The ethics committees of Sun Yat-sen Cancer Center approved our study. We conducted this study and observed the Helsinki declaration.
We included patients who were diagnosed as having primary duodenum adenocarcinoma by pathology. The exclusion criteria is as follows: (1) pathologically diagnosed with other types of tumours, such as gastrointestinal stromal tumour, neuroendocrine carcinoma and lymphoma; (2) patients with tumours of the distal common bile duct, pancreas or the ampulla of Vater; and (3) patients with incomplete clinicopathological factor information, with synchronous malignant tumours or patients who were lost to follow-up within three months. In total, 244 patients were enrolled in our study.
Data collection
At first diagnosis, we collected clinical data, including gender, age, abdominal pain, smoking history, family history of cancer, white blood cells (WBC), neutrophil, lymphocyte, monocyte, haemoglobin, platelet, albumin, LDH, TNM stage, pathological differentiation, endoscopy examination and imaging results. TNM stages were classified according to the 7th edition of the TNM staging system by the American Joint Committee on Cancer standards(AJCC). We measured the serum LDH after 2 months of therapy. We also included other prognostic factors, such as the platelet-lymphocyte ratio (PLR), lymphocyte-monocyte ratio (LMR), neutrophil-lymphocyte ratio (NLR), and prognostic nutritional index (PNI, PNI = 5 ×lymphocyte count (109/L) + 10 ×albumin (g/dL)). We regularly followed up patients by phone or using follow-up in the outpatient clinic. The last follow-up date was June 20th, 2017. The OS was the primary outcome and defined as the period from diagnosis to the date of death or to the date of the last follow-up.
Statistical analysis
We performed statistical analysis using SPSS (version 22.0; IBM Corp., Armonk, NY, USA), GraphPad Prism 6.0 (GraphPad Software Inc., La Jolla, CA, USA) and MedCalc software (version 11.4.20; MedCalc Software bvba, Acacialaan, Belgium). We considered a two-tailed P<0.05 as statistical significance. Cut-off points for the LDH, WBC, haemoglobin and platelet levels were determined using the upper limit of the normal levels, while cut-off values of NLR, PLR, PNI, and LMR were established using the receiver operating characteristic (ROC) curve analysis. The optimal cut-off value had the maximum Youden index (sensitivity + specificity - 1). We compared differences in the categorical factors between the groups using the Chi-square test and Fisher's exact test. Median values between the different groups were compared using the Mann-Whitney test. In the univariate analyses, we estimated the survival difference of the distinct variables using the Kaplan-Meier method (log-rank test) and identified significant factors. We subsequently brought these factors into the Cox proportional hazards regression model in multivariate analysis to distinguish the independent prognostic factors.
Results
Patient characteristics
A total of 244 patients with primary duodenum adenocarcinoma were included in our study. Baseline demographics are shown in Table 1. The median age was 57 years old with an interquartile range (IQR) of 49-63 years old. One hundred fifty-four (63.1%)patients were male and 90 (36.9%)patients were female. Ninety-five (38.9%) patients presented with abdominal pain at the first diagnosis. Fifty-two (21.3%) patients were diagnosed with a metastatic tumour and 192 (78.7%) patients had a non-metastatic tumour. Thirty-seven (15.2%), 86 (35.2%) and 69 (28.3%) patients were diagnosed at stages Ⅰ, Ⅱ and Ⅲ, respectively. One hundred sixty-four (67.2%) patients received radical surgery while 80 (32.8%) patients received palliative therapies, such as chemotherapy and radiotherapy. For all included patients, the 1-year, 2-year, 3-year and 5-year survival rates were 75.0%, 57.8%, 42.6% and 27.5%, respectively. For patients who received radical surgery, the 1-year, and 2-year, 3-year and 5-year survival rates were 90.2%, 74.4%, 57.3% and 35.4%, respectively. Eventually, 207 (84.9%) patients were classified into the normal LDH (≤245U/L) group, while 37 (15.1%) patients were in the higher LDH (>245U/L) group.
Table 1.
Baseline demographics of 244 patients with primary duodenum adenocarcinoma.
| Characteristic | No. of cases (%) |
|---|---|
| Age, years (median/IQR) | 57 (49-63) |
| Gender | |
| Male | 154 (63.1) |
| Female | 90 (36.9) |
| Abdominal pain | |
| Yes | 95 (38.9) |
| No | 149 (61.1) |
| Smoking history | |
| Yes | 56 (23.0) |
| No | 188 (77.0) |
| Family history of cancer | |
| Yes | 33 (13.5) |
| No | 211 (86.5) |
|
Laboratory parameters
(median/ IQR) |
|
| WBC (*109/L) | 7.4 (5.6-10.4) |
| Neutrophil (*109/L) | 5.1 (3.3-7.5) |
| Lymphocyte (*109/L) | 1.4 (1.1-1.8) |
| Monocyte (*109/L) | 0.5 (0.4-0.8) |
| Haemoglobin (g/L) | 108 (95-123) |
| Platelet (*109/L) | 259 (186-340) |
| Albumin (g/L) | 38 (34.5-41.3) |
| LDH (U/L) | 176 (153-216) |
| T category (AJCC 7th) | |
| T1 | 10 (4.1) |
| T2 | 61 (25.0) |
| T3 | 54 (22.1) |
| T4 | 119 (48.8) |
| N category (AJCC 7th) | |
| N0 | 128 (52.5) |
| N1 | 92 (37.7) |
| N2 | 24 (9.8) |
| M category (AJCC 7th) | |
| M0 | 192 (78.7) |
| M1 | 52 (21.3) |
| TNM (AJCC 7th) | |
| Ⅰ | 37 (15.2) |
| Ⅱ | 86 (35.2) |
| Ⅲ | 69 (28.3) |
| Ⅳ | 52 (21.3) |
| Radical surgery | |
| Yes | 164 (67.2) |
| No | 80 (32.8) |
| 1-year survival rate | 183 (75.0) |
| 2-year survival rate | 141 (57.8) |
| 3-year survival rate | 104 (42.6) |
| 5-year survival rate | 67 (27.5) |
IQR: Interquartile range; TNM: Tumour-node-metastasis; AJCC: American Joint Committee on Cancer; WBC: white blood cell; LDH: lactate dehydrogenase.
The median baseline serum LDH levels were significantly higher in patients with node metastases than those without (188.5U/L vs. 171.5U/L, P=0.01). The same can be said for those with stage Ⅲ-Ⅳ compared to those with stage Ⅰ-Ⅱ (190U/L vs. 171U/L, P=0.009), as well as those with poor differentiation compared to those with well & moderate differentiation (190U/L vs. 171U/L, P=0.018) (Figure 1).
Figure 1.
Baseline serum LDH levels were compared using the Mann-Whitney test in different subgroups. (A), Without node metastasis vs. with node metastasis; (B), TNM stage Ⅰ-Ⅱ vs. TNM stage Ⅲ-Ⅳ; (C), Well and moderate differentiation vs. poor differentiation.
The association between LDH and clinicopathological factors
We classified all patients into a series of subgroups according to the cut-off values of the different clinicopathological factors and analysed the potential correlations between these factors and the serum LDH level. We found that higher LDH level(>245U/L) was significantly associated with more distant metastasis, more node metastasis, poorer differentiation, more advanced TNM stage(Ⅲ-Ⅳ) and higher neutrophil to lymphocyte ratio (NLR)(P<0.05). There was a statistical trend between the higher LDH level and older age (>57 years old) (P=0.051). We did not find other significant associations between the LDH and other clinicopathological factors listed in Table 2 so far.
Table 2.
Correlation analysis for clinicopathologic variables in the normal LDH group and higher LDH group among the 244 primary duodenum adenocarcinoma patients.
| Variable | Normal LDH ≤ 245 U/L n (%) |
High LDH > 245 U/L n (%) |
P value |
|---|---|---|---|
| Total | 207 (84.9) | 37 (15.1) | |
| Age, years | 0.051 | ||
| > 57 | 92 (44.4) | 23 (62.2) | |
| ≤ 57 | 115 (55.6) | 14 (37.8) | |
| Gender | 0.584 | ||
| male | 129 (62.3) | 25 (67.6) | |
| female | 78 (37.7) | 12 (32.4) | |
| WBC | 0.315 | ||
| > 10 * 109/L | 53 (25.6) | 13 (35.1) | |
| ≤ 10 * 109/L | 154 (74.4) | 24 (64.9) | |
| Haemoglobin | 0.695 | ||
| > 120 g/L | 58 (28.0) | 9 (24.3) | |
| ≤ 120 g/L | 149 (72.0) | 28 (75.7) | |
| Platelet | 1 | ||
| > 300 * 109/L | 73 (35.3) | 13 (35.1) | |
| ≤ 300 * 109/L | 134 (64.7) | 24 (64.9) | |
| NLR | 0.022 | ||
| > 2.56 | 131 (63.3) | 31 (83.8) | |
| ≤ 2.56 | 76 (36.7) | 6 (16.2) | |
| PLR | 0.673 | ||
| > 267.5 | 47 (22.7) | 10 (27) | |
| ≤ 267.5 | 160 (77.3) | 27 (73) | |
| PNI | 0.216 | ||
| > 45.7 | 102 (49.3) | 14 (37.8) | |
| ≤ 45.7 | 105 (50.7) | 23 (62.2) | |
| LMR | 0.521 | ||
| > 4.7 | 44 (21.3) | 6 (16.2) | |
| ≤ 4.7 | 163 (78.7) | 31 (83.8) | |
| T category | 0.239 | ||
| 1-2 | 57 (27.5) | 14 (37.8) | |
| 3-4 | 150 (72.5) | 23 (62.2) | |
| N category | 0.012 | ||
| 0 | 116 (56.0) | 12 (32.4) | |
| 1-2 | 91 (44.0) | 25 (67.6) | |
| M category (M0/M1) | 0.031 | ||
| 0 | 168 (81.2) | 24 (64.9) | |
| 1 | 39 (18.8) | 13 (35.1) | |
| Degree of differentiation | 0.037 | ||
| Well and moderately differentiated | 144 (69.6) | 19 (51.4) | |
| Poorly differentiated | 63 (30.4) | 18 (48.6) | |
| TNM stage | 0.007 | ||
| Ⅰ-Ⅱ | 112 (54.1) | 11 (29.7) | |
| Ⅲ-Ⅳ | 95 (45.9) | 26 (70.3) |
LDH: lactate dehydrogenase; TNM: Tumour-node-metastasis; WBC: white blood cell; PLR: platelet-lymphocyte ratio; LMR: lymphocyte-monocyte ratio; PNI: prognostic nutritional index; NLR: neutrophil-lymphocyte ratio.
Survival analysis
The median survival of all the patients was 31.3 months. At the end of our study, a total of 148 patients died of primary duodenum adenocarcinoma and 96 patients had survived. Median survival of the patients with a normal LDH level(≤245U/L) was significantly longer than that of patients with a higher LDH level(>245U/L) (42.5 m vs. 16.3 m, P=0.02). The 1-year, 2-year, 3-year and 5-year survival rates were 77.8%, 60.4%, 44.9% and 28.5%, respectively, for patients in the normal LDH group, while the rates were 59.5%, 43.2%,29.7% and 21.6% for those in the higher LDH group, respectively. As shown in Table 3 and Figure 2, various factors, including age, node metastasis, distant metastasis, radical surgery, TNM stage, degree of differentiation, PNI and LDH were all significantly associated with OS(P<0.05). However, factors such as gender, T category (T1-2 vs. T3-4), NLR, PLR and LMR were not associated with survival in the current study (P>0.05). In the multivariate analysis, we brought all the significant variables determined by the univariate analyses into the Cox proportional hazards model. As the results showed, LDH, age and radical surgery were independent prognostic factors associated with the OS (HR=1.571, P=0.036 for LDH; HR=1.514, P=0.013 for age; HR=0.248, P<0.0001 for radical surgery, respectively).
Table 3.
Univariate and multivariate analyses of the prognostic factors for the overall survival of 244 patients with primary duodenum adenocarcinoma.
| Variable | Univariate | Multivariate | |||||
|---|---|---|---|---|---|---|---|
| Log-rank χ2 | P value | B | SE | HR | 95% CI | P value | |
| Gender (male/female) | 2.33 | 0.13 | |||||
| Age, years (> 57/≤ 57) | 12.09 | 0.0005* | 0.415 | 0.167 | 1.514 | 1.093 to 2.096 | 0.013 |
| T category (AJCC 7th) (T1/T2/T3/T4) | 4.97 | 0.17 | |||||
| N category (AJCC 7th) | 12.06 | 0.0024* | |||||
| N0 | NA | NA | 1 | Reference | NA | ||
| N1 | 0.230 | 0.540 | 1.258 | 0.4389 to 3.6083 | 0.670 | ||
| N2 | 0.582 | 0.589 | 1.789 | 0.5671 to 5.6424 | 0.324 | ||
| M category (AJCC 7th) (M0/M1) | 61.61 | < 0.0001* | 0.206 | 0.305 | 1.228 | 0.6777 to 2.2256 | 0.500 |
| Radical surgery (Yes/No) | 82.54 | < 0.0001* | -1.396 | 0.172 | 0.248 | 0.177 to 0.346 | <0.0001 |
|
TNM (AJCC 7th) (Ⅰ-Ⅱ/ Ⅲ-Ⅳ) |
14.94 | 0.0001* | -0.278 | 0.587 | 0.757 | 0.2411 to 2.3774 | 0.635 |
|
Degree of differentiation |
7.40 | 0.02* | |||||
| Well differentiation | NA | NA | 1 | Reference | NA | ||
| Moderate differentiation | -0.168 | 0.263 | 0.846 | 0.5067 to 1.4114 | 0.523 | ||
| Poor differentiation | 0.142 | 0.284 | 1.153 | 0.6625 to 2.0070 | 0.616 | ||
| NLR (> 2.56/≤ 2.56) | 3.32 | 0.07 | |||||
| PLR (> 267.5/≤ 267.5) | 0.89 | 0.34 | |||||
| PNI (> 45.7/≤ 45.7) | 4.98 | 0.03* | 0.023 | 0.179 | 1.023 | 0.7214 to 1.4505 | 0.899 |
| LMR (> 4.7/≤ 4.7) | 2.51 | 0.11 | |||||
| LDH (> 245/≤ 245) | 5.25 | 0.02* | 0.452 | 0.216 | 1.571 | 1.032 to 2.393 | 0.036 |
*All variables have a P value less than 0.05. We entered all the variables with P < 0.05 into multivariate analyses. NA: not applicable; TNM: Tumour-node-metastasis; AJCC: American Joint Committee on Cancer; WBC: white blood cell; PLR: platelet-lymphocyte ratio; LMR: lymphocyte-monocyte ratio; PNI: prognostic nutritional index; LDH: lactate dehydrogenase; NLR: neutrophil-lymphocyte ratio.
Figure 2.
Kaplan-Meier curves stratified by different variables in 244 patients with duodenum adenocarcinoma. OS curves stratified by (A), gender; (B), age; (C), T category; (D), N category; (E), M category; (F), Simplified TNM stage (Ⅰ-Ⅱ vs. Ⅲ-Ⅳ); (G), radical surgery; (H), LMR; (I), PNI; (J), NLR; (K), PLR; (L), LDH, and (M), Degree of pathological differentiation. TNM: tumour-node-metastasis; LMR: lymphocyte-monocyte ratio; PNI: the prognostic nutritional index; NLR: the neutrophil-lymphocyte ratio; PLR: the platelet-lymphocyte ratio; LDH: lactate dehydrogenase.
Based on the serum LDH values before and after 2 months of radical or palliative treatment, we divided these patients into 4 groups: the low-low group(n=180), low-high group(n=27), high-low group(n=17) and high-high group (n=20) (Table 4). The high-high group showed a similar OS with that of the low-high and high-low group (14.5 m vs 15.4 m vs 39.3 m, P>0.05), while the high-low group showed a similar OS with that of the low-low group (39.3 m vs 52.1 m, P=0.592). Patients with elevated LDH before or after 2 months of treatment (High-high/high-low/ low-high group) showed a significantly shorter OS than that of the low-low group (15.9 m vs 52.1 m, P=0.0003) (Table 4 and Figure 3).
Table 4.
Overall survival of the different groups based on serum LDH before and after treatment among the 244 primary duodenum adenocarcinoma patients in the present study.
| Serum LDH | Median overall survival(months) | 95% CI (months) |
p value |
|---|---|---|---|
| High-high(n=20) | 14.5 | 1.1-27.9 | |
| High-low(n=17) | 39.3 | 0-80.6 | Vs. high-high: 0.116 |
| Vs. low-low: 0.592 | |||
| Low-high(n=27) | 15.4 | 6-24.8 | Vs. high-high: 0.499 |
| Low-low(n=180) | 52.1 | 36.7-67.6 | Vs. high-high:<0.0001 |
| High-high/high-low/ low-high(n=64) | 15.9 | 11.7-20.1 | Vs. low-low: <0.0001 |
LDH: lactate dehydrogenase
Figure 3.
Kaplan-Meier curves for the OS of 244 patients with primary duodenum adenocarcinoma, which were stratified by the values of serum LDH before and after 2 months of radical or palliative treatments. (A) low-low group, low-high group, high-low group and high-high group (B) low-low group and High-high/high-low/ low-high group.
Comparison of LA-TNM and TNM staging systems
To improve the prognostic value of the TNM staging system, we integrated age and LDH into this system. Age > 57 and LDH > 245U/L were assigned 1 point while age ≤57 and LDH≤245U/L were assigned 0 points. Figure 4 A and B show the survival curves stratified by the TNM and LA-TNM staging system, respectively. The log-rank test showed that the P values of these two staging systems were both less than 0.001. A staging system had a better performance when it had a higher χ2 value and a lower Akaike information criterion(AIC) value in the likelihood ratio test (LRT). The LRT indicated that the χ2 value of the LA-TNM system was significantly higher than that of the TNM system (38.49 vs. 29.29, P<0.001). Moreover, the AIC value of the LA-TNM system was significantly lower than that of the TNM system (1437 vs. 1445.32, P<0.001) (Table 5). Therefore, the LA-TNM system was superior to the TNM system in predicting the overall survival of this cohort of patients in our study.
Figure 4.
Kaplan-Meier curves for the OS of 244 patients with primary duodenum adenocarcinoma, which were stratified by TNM (A), and LA-TNM (B). TNM: Tumour-node-metastasis; LA: LDH-Age.
Table 5.
Comparisons of the value of the TNM and LA-TNM systems in the predicting prognosis of the overall survival among the 244 primary duodenum adenocarcinoma patients in the present study.
| Stage system | Likelihood ratio test χ2 | AIC | p value |
|---|---|---|---|
| TNM | 29.29 | 1445.32 | < 0.01 |
| LA-TNM | 38.46 | 1437 | < 0.01 |
LA: LDH-Age; TNM: Tumour-node-metastasis; AIC: Akaike information criterion.
Discussion
Our team previously confirmed that the serum LDH level was an independent prognostic factor in locally advanced nasopharyngeal carcinoma and oesophageal squamous cell carcinoma 18,19. Other researchers have also confirmed LDH as an independent prognostic factor in pancreatic cancer and colorectal cancer 21, 23. However, the prognostic significance of LDH in primary duodenum adenocarcinoma has never been assessed hitherto. To the best of our knowledge, this is the first study exploring the prognostic value of LDH in a large cohort of patients with primary duodenum adenocarcinoma, and we found that elevated baseline serum LDH indicated a poor prognosis.
The tumour microenvironment is frequently hypoxic due to the rapid growth of the tumour cells 13. In hypoxic conditions, LDH can catalyse pyruvate into lactic acid and plays a key role in anaerobic glycolysis. HIF-1α and Myc are reported to transcriptionally activate key genes in tumour cellular energy metabolism, such as LDH, thereby raising anaerobic glycolysis and producing high levels of lactate 14,28. High levels of lactate will in turn enhance the expression of HIF-1α 14-15. By modulating metabolism and affecting the tumour microenvironment, the elevation of LDH is closely related to the tumour aggressiveness, metastasis, invasion and tumour burden 14-15. Consistent with this, our study suggested that a higher LDH level was significantly associated with more distant metastasis, node metastasis, poor differentiation and TNM stage Ⅲ-Ⅳ. On the other hand, a significantly higher median LDH level was observed in patients with a node metastasis, poor differentiation and a TNM stage of Ⅲ-Ⅳ. Researchers revealed that the expression of lactate dehydrogenase-5 (LDH-5) was associated with the hypoxia-inducible factor (HIF-1α) pathway, angiogenic factor production and poor prognosis in gastric cancer, but not in primary duodenum adenocarcinoma 15. Further work needs to be done to clarify if these aspects have some distinctive features in primary duodenum adenocarcinoma.
In addition to hypoxia, inflammation is another essential characteristic of tumours, leading to shorter survival in cancer patients 25-27. Systemic inflammation is correlated with hypoxia. Our study demonstrated that the LDH level (>245U/L) was significantly associated with a higher NLR.
Previous studies revealed that LDH, HIF-1α and Myc may be regulated by PI3K/Akt/TORC1/HIF pathway 28-30. In renal cell cancer carcinoma patients with high LDH levels, temsirolimus could provide survival benefits by inhibiting TORC128. Further studies need to be done to clarify whether temsirolimus will benefit primary duodenum adenocarcinoma patients with a higher level of LDH. It is worth mentioning that in colon cancer cells, researchers have found that LDH inhibitors could reduce production of lactate and reduce inflammation-induced changes 31.
An association between a high LDH level and tumour angiogenesis was determined by preclinical studies. HIF-1α was confirmed to increase the serum LDH level and tumour angiogenesis in vivo. Low levels of LDH may be predictive of the effectiveness of antiangiogenetic therapies 32-33. In a recent study, LDH was found to be capable of predicting the benefits from bevacizumab beyond progression in metastatic colorectal cancer. Patients with low serum LDH levels achieved significant PFS benefits from the bevacizumab maintenance treatment 32. In another study, the colorectal cancer patients with high serum LDH levels did not benefit from regorafenib 33. Moreover, a retrospective study included 21 unresectable or recurrent duodenum cancer patients to explore the value of the combination of chemotherapy with bevacizumab. The median OS of patients who received a bevacizumab-chemotherapy combination therapy was 21.9 months 34. The predictive role of LDH in primary duodenum adenocarcinoma patients receiving antiangiogenetic therapy needs to be further validated.
The cut-off values of LDH varied in the different studies, ranging from 170 to 450U/L. A recent meta-analysis explored the prognostic value of LDH in different types of tumours and classified LDH into four groups: <250U/L,250-300U/L,301-400U/L and >400 U/L. They drew the conclusion that differences among the distinct cut-off points were not statistically significant(P=0.99) 20. The ULN of LDH was 245U/L in our hospital, and was based on our classification of patients into either the normal LDH group(≤245U/L) or the high LDH group(>245U/L). Through analysis of 244 patients, we identified that patients in the higher LDH group had worse survival outcomes than those in the normal LDH group. Univariate analyses and multivariate analysis confirmed LDH as an independent prognostic indicator of OS. Higher LDH levels indicated more aggressive and unfavourable outcomes for primary duodenum adenocarcinoma patients. Other independent prognostic factors of OS in the current study were radical surgery and age. It is no doubt that radical surgery is the most important curative treatment for patients with primary duodenum adenocarcinoma. Abeezar et al analysed the prognostic factors of 137 primary duodenum adenocarcinoma patients and found that age and the number of involved lymph nodes were independent prognostic factors 9. We again confirmed age as a prognostic factor after multivariate analysis. A higher LDH level (>245U/L) indicates apparent hypoxia, exuberant angiogenesis and tumour aggressiveness, which might eventually lead to a worse OS.
To improve the prognostic value of the TNM staging system, we integrated age and LDH into this system to establish the LA-TNM staging system. LRT indicated that the LA-TNM system had a significantly larger χ2 value and a significantly smaller AIC value when compared with the TNM system in predicting the OS. LA-TNM outperformed the TNM system in predicting the overall survival in our selected cohort of patients.
There are several limitations of our study. First, this is a retrospective and single centre study. Although we strictly selected patients in accordance with our inclusion and exclusion criteria, selection bias may still exist. Second, we set the cut-off value of the serum LDH level as the ULN of 245U/L. As such, external validation cohorts from other hospitals will be needed for further confirmation of higher LDH level(>245U/L) as an independent risk factor for primary duodenum adenocarcinoma. Third, although the theoretical result is economical and convenient to perform, the low proportion of these patients that had a higher level of LDH may lead to limited clinical application. Additionally, a combination of serum biomarkers may be worth exploring. Large scale prospective randomized controlled clinical trials are warranted to further clarify the prognostic value of LDH in primary duodenum adenocarcinoma. Basic studies should be conducted to study the mechanisms of LDH and LDH inhibitors in primary duodenum adenocarcinoma.
In conclusion, our study provided the first demonstration that LDH is an independent prognostic factor for primary duodenum adenocarcinoma and that elevated baseline serum LDH levels indicate a poor prognosis.
Acknowledgments
This study was supported by the National High Technology Research and Development Program of China (863 Program), China(2015AA020103); National Natural Science Foundation (31600710, 81372374 and 81602066), Natural Science Foundation of Guangdong (No. 2014A030313205, 2014A030313146 and 2016A030313302) and the Project on the Integration of Industry, Education and Research of Guangdong Province (grant no. 2012B091100460), Fundamental Research Funds for the Central Universities (Grant number. 16ykpy25); and The third outstanding young talents training plan and medical scientist program of the Sun Yat-sen Cancer Center.
Abbreviations
- TNM
Tumour-node-metastasis
- AJCC
American Joint Committee on Cancer
- OS
overall survival
- ROC curve
receiver operating characteristics curve
- WBC
white blood cell
- PLR
platelet-lymphocyte ratio
- LMR
lymphocyte-monocyte ratio
- PNI
prognostic nutritional index
- LDH
lactate dehydrogenase
- NLR
neutrophil-lymphocyte ratio.
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