In Table 2, the sequence of oligonucleotide STX-Any r is incorrect. Please see the corrected Table 2 here.
Table 2. Oligonucleotides used in this study.
| Name | Sequence (5’- 3’) | Characteristics | Reference |
|---|---|---|---|
| Stx1-UP Stx1-LP |
CAGTTAATGTGGTGGCGAAGG ACTGCTAATAGTTCTGCGCATC |
gene stx1-A+B | [16] |
| S2Aup S2Alp |
ATGAAGTGTATATTATTTA TTCTTCATGCTTAACTCCT |
stx2A fragment | [9] |
| GK3 GK4 |
TCAGCCCCATACGATATAAG TGGAGTGGTGAATCCGTTAG |
stx2B fragment | [16] |
| UP378 LP378 |
GCGTTTTGACCATCTTCGT ACAGGAGCAGTTTCAGACAG |
stx2A fragment | [42] |
| Tc-5 Tc-3 |
TCAGCCCCATACGATATAAG TGGAGTGGTGAATCCGTTAG |
gene tet (Tc) |
[44] |
| Tc-int | TGTCGGAATGGACGATAT | binding 5’of tet cassette | [44] |
| RR46 LP RR46-UP |
GAGCTCTAAGGAGGTTAT GTGCAGTACTCATTCGTT |
gene Red recombinase in vector pKD46 | [44] |
| pBADf pBADr |
ATGCCATAGCATTTTTATCC GATTTAATCTGTATCAGG |
Confirmation of pBAD construct | Invitrogen |
| pGEM7up | TGTAATACGACTCACTAT | Confirmation of pGEM construct | Promega |
| RecX up RecAv lp | TCAGTCGGCAAAATTTCGC AGGTGCACTGAAAGCGGCTCG |
recA gene | This study |
| Tc-5RecX lp Tc-3RecAvup |
CTAACGGATTCACCACTCCACGCAGTAGCCTGACGACGCA CTTATATCGTATGGGGCTGAGGACCTGCGCGTATTCGCCA |
fragment of the tet gene for generation of ΔrecA mutant | This study |
| Tc-5RcsB Tc-3RcsC |
CTTATATCGTATGGGGCTGAGCGAATACCGAACAAGACTA CTAACGGATTCACCACTCCATCACTACCGCTCTGCCCTGC | fragment of the tet gene for ΔrcsBΔrcsC mutant |
This study |
| RcsAup YedPlp |
ATGTCAACGATTATTATGGAT TGGCTAATACCTGAGATGAT |
fragment between rscA and dsrA genes | This study |
| Tc-5RcsA Tc-3YedP |
CTTATATCGTATGGGGCTGACGCTGACTGTTAGAAGCATC CTAACGGATTCACCACTCCAATGTTTTCAATTCAACAACC |
fragment of the tet gene for ΔrcsAΔdsrA mutant | This study |
| CpxAup CpxPlp |
GAATAACGCAGAGCATTAC GAACTGACTGCCAGCGTTGA |
CpxAR phosphorelay system | This study |
| Tc-5CpxA Tc-3CpxP |
CTTATATCGTATGGGGCTGAAGTTGTGGAGTGAAGTGCTG CTAACGGATTCACCACTCCATGAAGCCTTCCATCTCGAG |
fragment of the tet gene for ΔcpxAΔcpR mutant | This study |
| BaeSup BaeRlp |
TGAAGTTCTGGCGACCCGGTAT CTAAACGATGCGGCAGGCGTCG |
BaeSR phosphorelay system | This study |
| Tc-5BaeS Tc-3BaeR |
CTTATATCGTATGGGGCTGACTGAAAGACAAAGCGATCAT TAACGGATTCACCACTCCAGCGTAGTAACCGACCGCACC |
fragment of the tet gene for ΔbaeSΔbaeR mutant | This study |
| STX-Any f STX-Any r STX-Any M |
ACGGACAGCAGTTATACCACTCT CTGATTTGCATTCCGGAACGT FAM- CCAGCGCTGCGACACG-NFQ |
Real-Time PCR for stx2 gene detection | [45] |
Reference
- 1.Imamovic L, Muniesa M (2012) Characterizing RecA-Independent Induction of Shiga toxin2-Encoding Phages by EDTA Treatment. PLoS ONE 7(2): e32393 https://doi.org/10.1371/journal.pone.0032393 [DOI] [PMC free article] [PubMed] [Google Scholar]
