Fig. 2. Cu chelation impairs myogenic differentiation. (A) Representative light micrographs of primary myoblasts derived from mouse satellite cells differentiated in media containing insulin (control), 30 μM TEPA, or 30 μM TEPA and 30 μM CuSO4 after 24 and 48 h. (B) Steady state mRNA levels of the myogenic markers Myog, MyhII, Acta1 and Ckm in primary myoblasts differentiated as indicated in panel (A). Data represent the mean of at least four independent biological replicates ±SD. **P < 0.01.
