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. 2017 Dec 12;10(2):290–303. doi: 10.1080/19420862.2017.1405203

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© 2018 The Author(s). Published by Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 9. — Dynamic of glycan species profile during a fed-batch process. A mAb3 expressing clone 5 was cultivated in a 14-day fed-batch process using media CDM-B and respective feeds. A: The glycan species abundance at different process timepoints, measured by RP-HPLC, is shown for sum of a-fucosylated (A-fuc) IgGs, sum of high mannose IgGs, sum of a-fucosylated complex-type IgGs, and sum of a-fucosylated hybrid-type IgG. B: The abundance of galactosylated IgGs and sum of GlcNAc-bisecting glycan species is shown.