Fig. 4.
H3K27me3 and H3K4me3 change in opposite directions at MIR156A and MIR156C during shoot development. (A,B) ChIP analysis of the abundance of H3K27me3 at MIR156A (A) and MIR156C (B) in the shoot apices of 1-, 2-, 3- and 5-week-old Col plants grown under short-day (SD) conditions. (C,D) ChIP analysis of the abundance of H3K4me3 at MIR156A (C) and MIR156C (D) in the shoot apices of 1-, 2-, 3- and 5-week-old Col plants grown under SD conditions. Data are the ratio of H3K27me3 or H3K4me3 relative to H3, and are the mean of three biological replicates±s.e.m. Graphs are aligned with the genomic structure of MIR156A and MIR156C, which is shown at the top of the figure. *P<0.05, one-way ANOVA. Black bars indicate the exons; gray boxes indicate the miR156 hairpin.