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Immunohistochemical characterization of Donor 1 MSC aggregates with incorporated microparticle-loaded transgenes. Tissue sections were stained for col I, II, and X (pink/red) with Fast Green Counterstain after A) 2 weeks and B) 4 weeks in vitro culture. Col I, collagen type I; Col II, collagen type II; Col X, collagen type X; MCM, mineral-coated hydroxyapatite microparticles; TGFβ1, transforming growth factor-beta 1; BMP2, bone morphogenetic protein-2. Scale bars, 100 μm.
