Figure 3. Representative images from patient-derived tumour organoids.

The four above panels are representing the human colon-derived organoids with different magnifications. Scale bars, 75 μm and 25 μm. The left two panels show the morphological alterations of the first patient’ (P1) a tumour derived organoid at different magnifications during days 4 to 14. The right two panels show another patient's derived tumour (P2) organoids grown and maintained for the same period. The lack of the intact crypt in tumour bulk is mainly due to the transformation of tumour cells as presented on day 0. Beside single cells, some other cells aggregated and formed clusters. Images in the first column, are captured by using a low magnification objective (1.25x) to focus mainly and to chase each specimen for subsequent organoids growth. However, producing sharp and quality 3D images using lower than 10x -magnification is very difficult. We have therefore used 10x objectives for columns 2 for each patient organoid.