Corrigendum to “Cytoprotective and Cytotoxic Effects of Rice Bran Extracts in Rat H9c2(2-1) Cardiomyocytes”

In the article titled “Cytoprotective and Cytotoxic Effects of Rice Bran Extracts in Rat H9c2(2-1) Cardiomyocytes” [1], errors in statistical analyses for inhibitory concentration (IC₅₀) have resulted in incorrect tabulations of data for both Tables 2 and 4. The corrected versions of both tables are as below.

Table 2.

The relative inhibitory concentration (IC₅₀) of RBE of BJLN and MR219. Data presented as mean ± standard deviation of three technical replicates (n = 3).

	BJLN		MR219
	Log (dose) (μg/mL)	Dose (μg/mL)	Log (dose) (μg/mL)	Dose (μg/mL)
Day 1 (24 hours)	1.808 ± 0.011	64.27	1.980 ± 0.013	95.56
Day 2 (48 hours)	1.775 ± 0.002	59.57	2.047 ± 0.026	111.40
Day 3 (72 hours)	1.800 ± 0.004	63.10	2.033 ± 0.029	108.00

Table 4.

Average IC₅₀ of H₂O₂ for H9c2(2-1) cells. The IC₅₀ value was determined from respective cell viability curves ( Figure 5) via GraphPad Prism (GraphPad Software Inc., USA). Data represent mean ± standard deviation of 3 (n = 3). ∗ denotes significantly different from negative control treated with media + 1% EtOH at P ≤ 0.05. Graphical representations of data were depicted in Figure 5.

	Average IC50 of H2O2 (μM)
	Log [H2O2]	H2O2
Control sample
Negative control (media + 1% EtOH)	2.433 ± 0.040	271.00
RBE
BJLN (25 μg/mL)	2.776 ± 0.028∗	597.20
BJLN (50 μg/mL)	1.954 ± 0.033∗	89.95
MR219 (50 μg/mL)	2.561 ± 0.035∗	364.20
MR219 (100 μg/mL)	2.158 ± 0.032∗	143.90

Accordingly, in the “Results” (Section 3.1), the text reading “Based on the results (Table 2), the IC₅₀ values of RBE of BJLN were in the range of 61.67 to 64.57 μg/mL over 24, 48, and 72 hours of incubation time.” should be corrected to “Based on the results (Table 2), the IC₅₀ values of RBE of BJLN were in the range of 59.57 to 64.27 μg/mL over 24, 48, and 72 hours of incubation time.”, and “Based on the results, the IC₅₀ values of MR219 RBE were in the range of 95.44 to 111.50 μg/mL over the three different incubation periods (Table 2).” should be corrected to “Based on the results, the IC₅₀ values of MR219 RBE were in the range of 95.56 to 111.40 μg/mL over the three different incubation periods (Table 2).”

In addition, in the “Results” (Section 3.3), the text reading “The positive effects were more distinctive with lower concentrations of RBE (BJLN: 25 μg/mL; MR219: 50 μg/mL) with observable increments in IC₅₀ of H₂O₂ (BJLN: 645.65 μM; MR219: 320.63 μM) (Table 4) when compared to negative control (316.23 μM). When the two extracts were compared, BJLN (25 μg/mL) extract outran MR219 (50 μg/mL) extract in terms of efficacy with a significant increment in IC₅₀ of H₂O₂ approximately twofold (645.65 μM) versus 1.4% (in approximation) when compared to negative control (316.23 μM).” should be replaced with “The positive effects were more distinctive with lower concentrations of RBE (BJLN: 25 μg/mL; MR219: 50 μg/mL) with observable increments in IC₅₀ of H₂O₂ (BJLN: 597.20 μM; MR219: 364.20 μM) (Table 4) when compared to negative control (271.00 μM). When the two extracts were compared, BJLN (25 μg/mL) extract outran MR219 (50 μg/mL) extract in terms of efficacy with a significant increment in IC₅₀ of H₂O₂ by approximately 2-fold (597.20 μM) versus 1.4-fold (in approximation) when compared to negative control (271.00 μM).”, and the text reading “Significant decrements in the IC₅₀ values of H₂O₂ were found for cell pretreated with 50 μg/mL BJLN (92.90 μM) and 100 μg/mL MR219 (171.79 μM) extracts when compared to negative control (316.23 μM) (Table 4). The higher concentrations of BJLN and MR219 extracts selected were near the range of IC₅₀ of both extracts (IC₅₀ of BJLN: 52.18 μg/mL to 73.09 μg/mL; IC₅₀ of MR219: 95.44 μg/mL to 111.50 μg/mL).” should be replaced with “Significant decrements in the IC₅₀ values of H₂O₂ were found for cell pretreated with 50 μg/mL BJLN (89.95 μM) and 100 μg/mL MR219 (143.90 μM) extracts when compared to negative control (271.00 μM) (Table 4). The higher concentrations of BJLN and MR219 extracts selected were near the range of IC₅₀ of both extracts (IC₅₀ of BJLN: 59.57 μg/mL to 64.27 μg/mL; IC₅₀ of MR219: 95.56 μg/mL to 111.40 μg/mL).”

An incorrect version of Figure 3 with missing graphical elements was published. The corrected version of Figure 3 with the inclusion of graphical elements is as shown below.

Figure 3.

Total contents of selected bioactive compounds in the RBE. Different letters on a bar represent significant differences at P ≤ 0.05 (Tukey's test).

Accordingly, Figure 5(b) presented in the original manuscript was also the incorrect version. The fourth datum point for MR219 (50 μg/mL) (grey dotted line) was incorrectly plotted. The correct version of the figure is as shown below with the corrected fourth datum point for MR219 (50 μg/mL) (grey dotted line).

Figure 5.

Effects of H₂O₂ inductions on cell viabilities of H9c2(2-1) cardiomyocytes pretreated with different concentrations of (a) BJLN RBE (25 μg/mL and 50 μg/mL) and (b) MR219 RBE (50 μg/mL and 100 μg/mL).