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. 2017 Dec 15;67(3):423–436. doi: 10.2337/db17-0736

Figure 1.

Figure 1

SRp55 is highly expressed in human pancreatic β-cells, and its depletion leads to increased β-cell apoptosis. A: Fluorescence microscopy of insulin and SRp55 in human islets (left panel) and in the human EndoC-βH1 cell line (right panel) shows staining of SRp55 (red), insulin (green), and nuclei (blue). B: mRNA expression of SRp55 in human islets, EndoC-βH1 cells, and a panel of normal human tissues was measured by quantitative RT-PCR (qRT-PCR) and normalized by the housekeeping gene β-actin. C and D: Human islets were transfected with siCTL or specific siRNA against SRp55 (siSR#1 and siSR#2) for 48 h. SRp55 KD levels were assessed by qRT-PCR (C), and apoptosis was evaluated by Hoechst/propidium iodide (PI) staining (D). E–I: EndoC-βH1 cells were transfected with control or specific siRNA against SRp55 for 48 h. SRp55 KD levels were assessed by qRT-PCR (E) and by Western blotting (F). Apoptosis of EndoC-βH1 cells after SRp55 KD was evaluated by Hoechst/PI staining (G) and by cleaved caspase 3 immunofluorescence (H and I). Scale bars = 1 µm. Results are the mean ± SEM of three to nine independent experiments. *P < 0.05, **P < 0.01, and ***P < 0.001 vs. siCTL (paired t test).