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. 2018 Feb 9;7:e33220. doi: 10.7554/eLife.33220

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© 2018, Szuperak et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Author response image 2. — Total sleep (A) and quantification of number of dividing NBs (B) shows that the relationship between proliferation rate and sleep is mixed. Expression of UAS-Ras1^V12or UAS-Alk in NBs (using worniu-GAL4) results in a comparable increase in NB division, but only UAS-Ras increases sleep. In contrast, expression of UAS-dp60 causes a dramatic reduction in dividing NBs, but sleep is increased. For sleep experiments, n=29, 41, 35, 22 larvae from left to right. For measurement of dividing NBs, n=18, 19, 14, 11 brains from left to right. ***P<0.0001. ANOVA with Tukey’s test. (We thank Dr. Sarah Siegrist for worniu-GAL4 and UAS-dp60).