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. 2016 Sep 19;139(11):2891–2908. doi: 10.1093/brain/aww228

Figure 2.

Figure 2

Five small molecules decrease levels of expanded ATXN3 in confirmation screens using 293.ATXN3Q81.Luc cells. (A) Representative anti-ATXN3 immunoblots show the efficacy of sodium salinomycin (Na Salinomycin), AM251, aripiprazole, clotrimazole and mifepristone to reduce the amount of mutant ATXN3 fusion protein after 48 h treatment of 293.ATXN3Q81Luc cells. Compounds were dissolved in DMSO except aripiprazole, which was dissolved in 1:1 DMSO/Tween-80. Quantification of bands corresponding to ATXN3Q81Luc and endogenous ATXN3 (endATXN3) is shown in B and C, respectively. Bars represent the mean percentage of each protein relative to vehicle-treated cells and normalized to α-tubulin (±SEM) in three independent experiments. Comparisons between cells treated with a specific compound concentration and cells treated with vehicle were performed using Student’s t-test with statistical significance, as indicated: *P < 0.05 and **P < 0.01.