Inhibitory effects of methamphetamine on mast cell activation and cytokine/chemokine production stimulated by lipopolysaccharide in C57BL/6J mice

Abstract

Previous studies have demonstrated that methamphetamine (MA) influences host immunity; however, the effect of MA on lipopolysaccharide (LPS)-induced immune responses remains unknown. Mast cells (MCs) are considered to serve an important role in the innate and acquired immune response, but it remains unknown whether MA modulates MC activation and LPS-stimulated cytokine production. The present study aimed to investigate the effect of MA on LPS-induced MC activation and the production of MC-derived cytokines in mice. Markers for MC activation, including cluster of differentiation 117 and the type I high affinity immunoglobulin E receptor, were assessed in mouse intestines. Levels of MC-derived cytokines in the lungs and thymus were also examined. The results demonstrated that cytokines were produced in the bone marrow-derived mast cells (BMMCs) of mice. The present study demonstrated that MA suppressed the LPS-mediated MC activation in mouse intestines. MA also altered the release of MC cytokines in the lung and thymus following LPS stimulation. In addition, LPS-stimulated cytokines were decreased in the BMMCs of mice following treatment with MA. The present study demonstrated that MA may regulate LPS-stimulated MC activation and cytokine production.

Introduction

Methamphetamine hydrochloride (MA) is a widely abused psychostimulant with an estimated 35 million users worldwide; thus, it has become a public health problem (1). A number of animal and clinical studies have demonstrated that MA abuse induces immunosuppressive effects, thereby increasing susceptibility to infectious diseases (2). Lipopolysaccharide (LPS), an immunostimulant, may mediate the immune response associated with gram-negative bacterial infection. Although previous studies have demonstrated the detrimental effect of MA on host immunity (3,4), the effect of MA following stimulation with LPS on the immune response has not yet been described.

It has been demonstrated that mast cells (MCs) serve an important role in innate and acquired immune responses (5,6), such that certain diseases are associated with changes in the number of MCs at affected sites (7,8). MCs are abundant at the borders of the external environment, including the intestinal mucosa where MCs function as sentinel cells during immune defense (9,10). Cluster of differentiation 117 (CD117, also known as c-kit) is a primary receptor and MCs marker and it has been demonstrated that a loss-of-function mutation in c-kit causes MCs deficiency in mice (11). The type I high affinity immunoglobulin E receptor (FcεRI) is an another receptor and marker of MCs that excites FcεRI and activates MCs (12). It has been demonstrated that LPS induces rodent MCs to secrete a variety of cytokines, including tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-4, IL-6, IL-10, IL-13 and chemokine ligand-5 (CCL-5) (13,14). MC-mediated cytokine production is often greater than that from other immunocytes, including macrophages and T cells (15,16). In addition, MCs serve an important role in the lung (17); indeed, thymic MCs have been implicated in infection-induced thymus involution (18). However, it is remains unknown whether MA modulates MCs activation and the subsequent production of cytokines stimulated by LPS.

The present study assessed the effect of MA on MC activation and release of cytokines stimulated by LPS in C57BL/6J mice. The expression of CD117 and FcεRI was measured in mouse intestines and it was demonstrated that MCs released cytokines in the lung and thymus tissues following treatment with MA and LPS stimulation. To further verify the effect of MA on the response of MCs mediated by LPS, LPS-stimulated cytokine production following MA treatment in mouse bone marrow-derived mast cells (BMMCs) was examined. The results of the present study demonstrate that MA may regulate MC activation and LPS-stimulated cytokine production.

Materials and methods

Animals

A total of 48 C57BL/6J mice aged 6-week-old (weighing ~20 g; 24 males: 24 females) were purchased from the Laboratory Animal Department of Xi'an Jiaotong University Medical School (Xi'an, China). C57BL/6J mice were housed in a specific pathogen-free facility (temperature, 22±3°C; relative humidity, 60±5%) maintained on a 12-h light/dark cycle. All the mice had free access to food and water. Experiments were approved by the Animal Ethics Committee of Xi'an Jiaotong University and all treatment procedures were performed in accordance with the guidelines of the Institutional Animal Care and Use Committee of Xi'an Jiaotong University.

Reagents

MA was purchased from the National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China). MA was dissolved in sterile 0.9% physiological saline and injected intramuscularly (i.m.) at a dose of 5 mg MA/kg. LPS (derived from Escherichia coli; serotype O55:B5; Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) was dissolved in sterile saline and injected i.m. at a dose of 150 µg/kg.

Animal treatments

The 6-week-old sex-matched C57BL/6J mice were randomly divided into 4 groups (n=12), including a normal saline (NS) NS+NS group (control), a MA+NS group, an NS+LPS group and a MA+LPS group. Mice received four i.m. injections of either 5 mg/kg MA or NS at 2 h intervals. Mice then received one i.m. injection of either LPS (150 µg/kg) or NS 24 h following the first MA injection. The 5 mg/kg dose of MA was selected based on the results of a previous study (19) and a preliminary experiment in the present study indicated that 150 µg/kg LPS was the most appropriate dose. Mice were sacrificed by CO₂ asphyxiation and tissues, including lung and thymus tissues, were obtained for histological analysis and cytokine measurement.

Immunohistochemistry

Intestine tissues were fixed in 10% formalin solution at room temperature for 24 h, and then samples were embedded in paraffin and 5-µm thick paraffin sections were prepared. The paraffin sections were deparaffinized and rehydrated in a descending alcohol series. Antigen retrieval was performed using a highly compressed heating method in a citrate buffer solution (95°C, 5–10 min). Endogenous peroxidase activity was blocked using a solution of methanol-0.3% H₂O₂ incubated for 30 min at room temperature. Slides were incubated with the rabbit anti-mouse polyclonal antibodies for CD117 and FcεRI (Beijing Bo Orson Biological Technology Co., Ltd., Beijing, China; dilutions, 1:100 and 1:200, respectively; cat. nos. bs-0672 and bs-13167R, respectively) overnight at 4°C. Slides were then washed three times with PBS (pH 7.4) and incubated with a secondary antibody (anti-rabbit immunoglobulin G; dilution, 1:500; cat. no. bs-0295M; Beijing Bo Orson Biological Technology Co., Ltd.) for 2–3 h at room temperature. Finally, 3,3′-diaminobenzidine (Dr. Wuhan's Biological Engineering Co., Ltd., Wuhan, China) was used for coloration at room temperature for 5 min. The chromogenic reaction was monitored every 3 min using an optical microscope. Following washing, sections were air-dried, dehydrated in ascending concentrations of ethanol, cleared with xylene and mounted under a cover slip with Permount. A total of 10 random fields per slide were examined and analyzed. The images were captured using a microscope (Leica microsystem GmbH, Wetzlar, Germany).

Reverse transcription-quantitative polymerase chain reaction (RT-qPCR)

Total RNA was extracted from the lungs and thymus of mice and subsequently purified using a TRIzol kit (Invitrogen, Thermo Fisher Scientific Inc., Waltham, MA, USA). Nucleic acid concentration and purity (A260/A280) was measured using a microplate instrument. Residual genomic DNA was removed by incubation with RNase-free deoxyribonuclease (Takara Bio, Inc., Otsu, Japan). Reverse transcription was performed using a PrimeScript^™ RT reagent kit (Takara Bio, Inc.) following the manufacturer's protocol. The resulting cDNA was subjected to qPCR using a Stratagene Mx 3005p real-time PCR Detection system (Agilent Technologies Inc., Santa Clara, CA, USA) using SYBR Green II (Takara Bio, Inc.) as a double-strand DNA-specific dye to quantify the expression of TNF-α, IL-1β, IL-6, IL-10, IL-4, IL-13 and CCL-5 in the lung and thymus of mice. The primer sequences were as follows: IL-1β forward, (F) 5′-GTCACAAGAAACCATGGCACAT-3′ and reverse, (R) 5′-GCCCATCAGAGGCAAGGA −3′; IL-4 F, 5′-ACGGAGATGGATGTGCCAAAC-3′ and R, 5′-AGCACCTTGGAAGCCCTACAGA-3′; IL-6 F, 5′-CTGCAAGAGACTTCCATCCAGTT-3′ and R, 5′-AGGGAAGGCCGTGGTTGT-3′; IL-10 F, 5′-GCCAGAGCCACATGCTCCTA-3′ and R, 5′-GATAAGGCTTGGCAACCCAAGTAA-3′; IL-13 F, 5′-CGGCAGCATGGTATGGAGTG-3′ and R, 5′-ATTGCAATTGGAGATGTTGGTCAG-3′; TNF-α F, 5′-GGCTGCCCCGACTACGT-3′ and R, 5′-ACTTTCTCCTGGTATGAGATAGCAAAT-3′; CCL-5 F, 5′-GGAGTATTTCTACACCAGCAGCAAG-3′ and R, 5′-GGCTAGGACTAGAGCAAGCAATGAC-3′; and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) F, 5′-GCACCGTCAAGGCTGAGAAC-3′ and R: 5′-TGGTGAAGACGCCAGTGGA-3′. All primers were synthesized by Bao Bioengineering Co., Ltd. (Dalian, China). The thermo cycling conditions of qPCR were as follows: Initial denaturation at 95°C for 30 sec; followed by 40 cycles at 95°C for 5 sec and 60°C for 30 sec; 1 cycle at 95°C for 60 sec, 55°C for 30 sec, and 95°C for 30 sec. Following the completion of qPCR, specificity was assessed using a melting curve analysis. The results were quantified using the 2^−ΔΔCq method (20). GAPDH was utilized as a reference gene.

Cytokine analysis of lung and thymus

The lungs and thymus of mice were homogenized at 4°C using tissue protein extraction reagent (Xi'an FengZu Biotechnology Co., Ltd., Xi'an, China) with a complete mini protease inhibitor cocktail and complete mini phosphatase inhibitor cocktail tablets (Roche Applied Science, Pleasanton, CA, USA), using 1 inhibitor tablet per 10 ml tissue protein extraction reagent. Tissue homogenates were centrifuged at 12,000 × g for 15 min at 4°C. The total protein concentration in the supernatants of lung and thymus homogenates was determined using a BCA kit (Zhuhai Jian Kangyuan Biopharmaceutical Co., Jian Kangyuan Group Corporation, Guangdong, China). Supernatants were then diluted using a tissue protein extraction reagent to a final protein concentration of 500 µg/ml and stored at 80°C until further use. Cytokines TNF-α, IL-1β, IL-6, IL-10, IL-4, IL-13 and CCL-5 in the supernatants were measured using ELISA (eBioscience; Thermo Fisher Scientific Inc., Waltham, MA, USA; cat. nos. BMS607-3, BMS6002, BMS603-2, BMS614-2, BMS613, BMS6015 and BMS6009INST, respectively) following the manufacturer's protocol.

BMMC preparation and cytokine measurements

BMMCs were obtained from the femurs of 6-week-old C57BL/6J mice, following a previously described protocol (21). Cells were cultured at 37°C in RPMI 1640 medium (Gibco; Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) (Gibco; Thermo Fisher Scientific, Inc.), 10 ng/ml IL-3, 10 ng/ml stem cell factor (SCF), 2 mM L-glutamine, 1 mM sodium pyruvate, 1 mM HEPES, 50 µM 2-mercaptoethanol, 100 U/ml penicillin and 100 µg/ml streptomycin. IL-3 and SCF were purchased from PeproTech Inc. (Rocky Hill, NJ, USA). After 4 weeks, flow cytometry was used to identify whether BMMCs were composed of >95% MCs. BMMCs were then incubated with fluorescence-labeled antibodies, including anti-CD117-flourescein isothocyanate (FITC; dilution, 1:100; cat. no. 553354; BD Biosciences Franklin Lakes, NJ, USA) and anti-FcεR1-APC (dilution, 1:200, cat. no. 17-5898-82; eBioscience, Thermo Fisher Scientific Inc.) for 1 h at 4°C.

BMMCs from C57BL/6J mice were treated with 100 µM/l MA and 1 µg/ml LPS for 24 h at 37°C. The concentration of TNF-α, IL-6, IL-4, IL-13 and CCL-5 cytokines present in the supernatants was then quantified using ELISA kits (eBioscience, Thermo Fisher Scientific Inc.; cat. nos. BMS607-3, BMS603-2, BMS613, BMS6015 and BMS6009INST) according to the manufacturer's protocol.

Statistical analysis

All the analysis was performed using SPSS software version 15.0 (SPSS, Inc., Chicago, IL, USA). One-way analysis of variance was used to determine the difference among groups. Comparisons of all pairs were completed using the Turkey-Kramer test. Data were expressed as the mean ± standard error of the mean and P<0.05 was considered to indicate a statistically significant difference.

Results

MA suppresses LPS-stimulated MC activation in the intestines of C57BL/6J mice

C57BL/6J mice received four i.m. injections of MA (5 mg/kg) or saline and were then injected with LPS or saline 24 h following the first MA injection. Fig. 1 presents the results of immunohistochemical staining for CD117⁺ and FcεRI⁺ in C57BL/6J mice. NS+LPS mice exhibited a significant increase in intestinal CD117⁺ and FcεRI⁺ compared with the NS+NS group (P<0.05; Fig. 1B and D). No effect on CD117⁺ or FcεRI⁺ cells was identified in MA+NS treated mice. However, it was demonstrated that significantly fewer intestinal CD117⁺ and FcεRI⁺ cells were present in MA+LPS treated mice, compared with mice that received NS+LPS (P<0.05; Fig. 1B and D).

Figure 1.

Effect of MA on LPS-stimulated MC activation in the intestines of C57BL/6J mice. (A) Anti-CD117 and (C) FcεRI immunoreactivity of intestines from C57BL/6J mice. Brown stained cells were defined as CD117 or FcεRI-positive cells. Stained sections were examined under a light microscope (magnification, ×400). Scale bar=50 µm. Quantification of immunohistochemical staining for (B) anti-CD117 (D) FcεRI. Data are expressed as the mean ± standard error of the mean (n=12). *P<0.05 vs. NS+NS group; ^#P<0.05 vs. NS+LPS group. MA, methamphetamine; LPS, lipopolysaccharide; CD117, cluster of differentiation 117; FcεRI, type I high affinity immunoglobulin E receptor; NS, normal saline.

MA suppresses LPS-stimulated production of inflammatory cytokines in the lungs of C57BL/6J mice

Mice treated with NS+LPS exhibited a significant increase in the mRNA and protein levels of the pro-inflammatory cytokines TNF-α, IL-1β and IL-6 in the lungs, compared with NS+NS mice (P<0.05; Fig. 2). MA+NS treatment had no effect on cytokine production. However, the mRNA and protein levels of all the pro-inflammatory cytokines were significantly decreased in the MA+LPS group, compared with mice in treated with NS+LPS (P<0.05; Fig. 2). NS+LPS treatment induced a significant increase in IL-10 mRNA and protein expression (P<0.05; Fig. 2). No significant difference in IL-10 production was identified between the MA+NS and the NS+NS groups; however, MA treatment significantly suppressed the LPS-mediated increase in IL-10 mRNA and protein expression (P<0.05; Fig. 2).

Figure 2.

Effect of MA on LPS-stimulated cytokine expression in the lungs of C57BL/6J mice. C57BL/6J mice were treated with four doses of NS or 5 mg/kg MA at 2 h intervals, followed by 150 µg/kg saline or LPS for 24 h. Lungs were then harvested and analyzed for cytokine (A) protein expression using ELISA or (B) mRNA expression using reverse transcription quantitative polymerase chain reaction. Data are expressed as the mean ± standard error of the mean (n=12). *P<0.05 vs. NS+NS group; ^#P<0.05 vs. NS+LPS group. MA, methamphetamine; LPS, Lipopolysaccharide; NS, normal saline; TNF-α, tumor necrosis factor-α; IL, interleukin; CCL-5, chemokine ligand-5.

MCs increase in number following the T helper 2 (Th2) response. Therefore, the effect of MA on LPS-stimulated Th2 cytokine/chemokine production in the lung was assessed. NS+LPS treatment significantly increased the mRNA and protein expression of the Th2 cytokines/chemokines IL-4, IL-13 and chemokine ligand-5 (CCL-5) in the lungs of mice (P<0.05; Fig. 2). MA+NS treatment had no significant effect on the production of Th2 cytokines/chemokines at either level in the lungs of mice. However, the mRNA and protein levels of Th2 cytokines/chemokines were significantly decreased in MA+LPS mice, compared with mice that received NS+LPS (P<0.05; Fig. 2).

MA suppresses the LPS-stimulated production of inflammatory cytokines in the thymus of C57BL/6J mice

NS+LPS treatment significantly increased the production of the pro-inflammatory cytokines TNF-α, IL-1β and IL-6 at the mRNA and protein level in the thymus of mice compared with the NS+NS group (P<0.05; Fig. 3A and B). No significant differences were identified in the mRNA or protein expression of thymic pro-inflammatory cytokines between mice treated with MA+NS and those treated with NS+NS. However, the mRNA and protein levels of the pro-inflammatory cytokines were significantly reduced in the thymus of the mice treated with MA+LPS compared with the NS+LPS group (P<0.05; Fig. 3).

Figure 3.

Effect of MA on LPS-stimulated cytokine expression in the thymus of C57BL/6J mice. C57BL/6J mice were treated with four doses of NS or 5 mg/kg MA at 2 h intervals, followed by 150 µg/kg saline or LPS for 24 h. The Thymus was harvested and analyzed for cytokine (A) protein expression using ELISA or (B) mRNA expression by reverse transcription quantitative polymerase chain reaction. Data are expressed as the mean ± standard error of the mean (n=12). *P<0.05 vs. NS+NS group; ^#P<0.05 vs. NS+LPS group. MA, methamphetamine; LPS, lipopolysaccharide; NS, normal saline; TNF-α, tumor necrosis factor α; IL, interleukin; CCL-5, chemokine ligand-5.

NS+LPS treatment significantly increased the mRNA and protein levels of the anti-inflammatory cytokine IL-10 in the thymus of mice (P<0.05; Fig. 3). MA+NS treatment alone had no effect on thymic IL-10 mRNA or protein expression. However, MA treatment significantly suppressed the LPS-stimulated increase in IL-10 mRNA and protein expression in the thymus of mice compared with the NS+NS group (P<0.05; Fig. 3).

NS+LPS treated mice exhibited a significant increase in the production of the Th2 cytokine/chemokines IL-4, IL-13 and CCL-5 at the mRNA and protein levels in the thymus of mice (P<0.05; Fig. 3). No difference in the expression of Th2 cytokines/chemokine was observed between MA+NS treated mice and the NS+NS group. However, the expression of Th2 cytokine/chemokine mRNA and protein was significantly reduced in the group treated with MA+LPS, compared with mice that received NS+LPS treatment (P<0.05; Fig. 3).

MA suppresses the LPS-stimulated inflammatory cytokine production in the BMMCs of C57BL/6J mice

To verify the suppressive effects of MA on LPS-stimulated cytokine production, BMMCs were cultured and supernatant cytokine levels were measured using ELISA. BMMCs produced significantly higher levels of TNF-α, IL-6, IL-4, IL-13 and CCL-5 in LPS-treated mice compared with NS+NS mice (P<0.05; Fig. 4). However, a significant decrease in cytokine/chemokine production was identified in the BMMCs of mice in the MA+LPS group compared with the LPS group (P<0.05; Fig. 4). IL-1β and IL-10 were not measured in BMMCs, as the concentrations were too low.

Figure 4.

Effect of MA on LPS-stimulated cytokine production in the BMMCs of C57BL/6J mice. BMMCs were treated with NP, MA and/or LPS at the indicated concentrations (MA, 100 µM; LPS, 1 µg/ml; MA+LPS, 100 µM MA+1 µg/ml LPS) for 4 h or 24 h at 37°C. Following treatment, cytokine concentrations in cell supernatants were evaluated using ELISA. Data are expressed as the mean ± standard error of the mean of four samples. *P<0.05 vs. NS+NS group at the same time-point; ^#P<0.05 vs. LPS group at the same time-point; ^##P<0.01 vs. NS+LPS group at the same time-point. MA, methamphetamine; LPS, Lipopolysaccharide; BMMCs, bone marrow-derived mast cells; TNF-α, tumor necrosis factor α; IL, interleukin; CCL-5, chemokine ligand-5.

Discussion

MA is a potent stimulant of the central nervous system and its abuse causes severe psychological and physical effects. Previous studies have revealed that MA negatively impacts immune responses, which may contribute to the higher rate and rapid progression of certain infections found in drug abusers (22,23). The present study therefore assessed the effects of MA on MC activation and cytokine/chemokine production in C57BL/6J mice that received LPS stimulation.

The results demonstrated that MA treatment suppressed LPS-mediated MC activation in the intestines of C57BL/6J mice. MCs are concentrated at interfaces between the host and environment, including the intestinal tract, where they limit the spread of invading pathogens (24). MCs are considered to function as effecter cells during the innate and adaptive immune responses (25–27). CD117 and FcεRI are primary MC surface receptors associated with MC activation (7,12,28–30). The present study demonstrated that MA+LPS treatment significantly decreased the expression of intestinal CD117 and FcεRI. However, the effect of MA and LPS on MCs themselves was not assessed in the present study. Therefore, further experiments should be conducted to investigate the expression profiles of MC cytokines stimulated with varying doses of LPS and MA.

Previous studies have demonstrated that LPS induces MCs to secrete cytokines, thus promoting the innate and adaptive immune responses (31,32). The most studied MC derived cytokine in the innate immune response is TNF-α, which induces the early influx of neutrophils and clearance of pathogens (33–35). It has been demonstrated that IL-6 produced by MCs increases the survival rates of patients with Klebsiella pneumoniae and sepsis by stimulating neutrophil activity (36). IL-10 is an anti-inflammatory cytokine which suppresses the synthesis of inflammatory cytokines, including TNF-α and IL-6 (37). It has been demonstrated that MCs can mediate negative immunomodulatory functions by producing IL-10 in response to chronic irradiation with UVB light (38). In addition, certain MC derived Th2-type cytokines, including IL-4 and IL-13, influence B-cell development and function (11). MCs are also an important source of chemokines, including CCL5, which is involved in Th2-type responses (10). The results of the present study demonstrate that MA treatment suppresses MC derived, LPS-stimulated, inflammatory cytokine production in the lungs and thymus of C57BL/6J mice. It was also revealed that MA has a suppressive effect on the production of LPS-stimulated inflammatory cytokines in the BMMCs of mice. The results indicated that MA abuse leads to immunosuppressive effects, which may increase the risk of infection. However, the present study did not assess the effect of MA on other immune cells. Further studies are required to improve understanding regarding the effects of MA on immune function.

In conclusion, the present study demonstrated that MA may be involved in the regulation of LPS-stimulated MCs activation and cytokine production. This may be responsible for the immune dysfunction and increased susceptibility to infectious diseases associated with MA abuse. Further studies are required to explore the mechanism underlying the immunomodulatory effects of MA.

Glossary

Abbreviations

MA

methamphetamine

LPS

lipopolysaccharide

MCs

mast cells

NS

normal saline

BMMCs

bone marrow-derived mast cells

SCF

stem cell factor

TNF-α

tumor necrosis factor-α

IL

interleukin

CCL-5

chemokine ligand-5