| Delivery |
Bathing, feeding, injection, transfection, transduction, transgenic |
Injection, transfection, transduction, transgenic |
| Mode of action |
RISC-induced mRNA degradation |
DSB triggered InDel formation |
| Transcriptional regulator recruitment |
| Specificity |
19-bp homology |
20-bp homology |
| Tolerates up to 10 mismatches |
Tolerates up to 3 mismatches |
| Side effect prone |
|
| Efficacy |
Strong in C. elegans
|
Null alleles |
| Strong in D. melanogaster
|
Highly efficient in many organisms across almost all domains |
| Weaker in H. sapiens
|
|
| Applications |
Pooled and arrayed, uni- and multivariate screening |
Pooled, uni- and multivariate screening |
| Single gene tests |
Single gene tests |
