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. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: Ocul Surf. 2017 Nov 4;16(1):58–69. doi: 10.1016/j.jtos.2017.11.002

Review. The diagnosis of limbal stem cell deficiency

Qihua Le a,b, Jianjiang Xu b, Sophie X Deng a,*
PMCID: PMC5844504  NIHMSID: NIHMS919758  PMID: 29113917

Abstract

Limbal stem cells (LSCs) maintain the normal homeostasis and wound healing of corneal epithelium. Limbal stem cell deficiency (LSCD) is a pathologic condition that results from the dysfunction and/or an insufficient quantity of LSCs. The diagnosis of LSCD has been made mainly based on medical history and clinical signs, which often are not specific to LSCD. Methods to stage the severity of LSCD have been lacking. With the application of newly developed ocular imaging modalities and molecular methods as diagnostic tools, standardized quantitative criteria for the staging of LSCD can be established. Because of these recent advancements, effective patient-specific therapy for different stages of LSCD may be feasible.

Keywords: Anterior-segment optical coherence tomography, Conjunctival marker, Diagnosis, Impression cytology, In vivo confocal microscopy, Limbal stem cell deficiency, Molecular marker

1. Introduction

Limbal stem cells (LSCs) are adult stem cells that further differentiate into corneal epithelium. Functional LSCs are essential for maintaining the integrity of the corneal surface and transparency of the cornea. The limbus is the transition area between the transparent cornea and the opaque sclera [1]. Normal limbus and LSCs act as a barrier against the invasion of conjunctival epithelial cells onto the cornea [24]. In the past four decades, most studies of LSCD have focused on the biology of LSCs and their function in maintaining the regeneration of the corneal epithelium under healthy and pathologic conditions.

LSCs reside in the basal layer of epithelium within the limbal area [5, 6]. Studies have provided direct evidence of LSC survival and self-maintenance at the limbus by using transgenic mice that expressed ubiquitous green fluorescent protein [7, 8]. The palisades of Vogt, a limbal structure that is shown to harbor a high density of LSCs, provide the niche microenvironment that is necessary for the survival and function of LSCs, and maintenance of their stemness. It might represent the collective influence of coexisting local stromal cells, the extracellular matrix, local vasculature and soluble growth factors [4, 6, 912]. LSCs are also found outside of the palisades of Vogt in adult and fetal human eyes, including limbal epithelial crypts and limbal epithelial pit [7, 13, 14].

Direct damage to LSCs and/or the destruction of their niche microenvironment leads to limbal stem cell deficiency (LSCD). As a result, the barrier function of the limbus is compromised, and the corneal epithelium is replaced with conjunctival epithelial cells, which is the hallmark of LSCD. Neovascularization could occur within the corneal epithelium and stroma, and the cornea becomes opaque eventually, leading to vision loss and blindness [3, 15].

Accurate diagnosis of LSCD is crucial because appropriate treatments can prevent progression of the condition and further damage to the ocular surface. For example, penetrating keratoplasty cannot restore sight to an eye blinded by LSCD before functional LSCs are restored [16]. In the past several decades, diagnosis of LSCD was predominantly made based on the patient’s medical history and clinical signs. However, inherent limitations are associated with the interpretation of clinical signs [17]. For instance, the presence of a fibrovascular pannus may be caused by previous infectious keratitis rather than LSCD. Moreover, there is no consensus on the methods to stage the severity of LSCD. With the application of newly developed ocular imaging techniques and progress in identifying molecular diagnostic markers and developing new tests based on these markers, the diagnosis of LSCD is coming into a new era.

To review the recent advances in the diagnosis of LSCD, we performed a systematic search on PubMed for English language literature published before March 31, 2017. The following combined search terms were used: “limbal stem cell deficiency” AND “diagnosis,” “limbal stem cell deficiency” AND ”in vivo confocal microscopy,” “limbal stem cell deficiency” AND “optical coherence tomography,” “limbal stem cell deficiency” AND “impression cytology.” Publications that are not related to the diagnosis of LSCD, or are published in non-English language were excluded. We collected data on diagnostic methods (clinical findings, in vivo confocal microscopy, anterior segment optical coherence tomography [OCT], impression cytology, or detection for various epithelial molecular markers). This literature review summarizes the diagnostic methods currently used in the diagnosis of LSCD and reports recent advances in the diagnosis and classification of LSCD. Based on these findings, a diagnostic approach for LSCD is proposed in the near future to overcome the limitations using the current diagnostic methods. We hope that this review will further emphasize the need to include objective testing using in vivo imaging modalities.

2. Etiology

The two pathologic mechanisms of LSCD are the direct destructive loss of LSCs and the loss of the limbal microenvironment/niche needed for LSC survival. The delicate niche of LSCs plays an important role in maintaining the LSC pool. Pathologic processes leading to either the direct loss of the LSC pool or the dysfunction of the limbal niche can result in the same phenotype of LSCD.

The etiology of LSCD can be primary, resulting from genetic mutations that lead to LSC dysfunction or destruction. The known causes of primary LSCD include aniridia [18, 19], congenital epidermal dysplasia [20, 21], dyskeratosis congenital [22], keratitis associated with multiple endocrine deficiencies [3], Turner syndrome [23], lacrimo-auriculo-dento-digital (LADD) syndrome [24], and xeroderma pigmentosum [25].

LSCD can also be secondary, resulting from external factors that directly destroy LSCs, damage the stem cell niche, or both. Common causes include chemical or thermal injuries [5, 15, 2628]; chronic inflammation and cicatricial process from mucous membrane pemphigoid [28, 29], Stevens-Johnson syndrome [30, 31], graft-versus-host disease [32] and chronic limbitis [33, 34]; iatrogenic injury caused by ocular surgeries, radiation, cryotherapy, or systemic chemotherapy [28, 3538]; drug-induced toxicity such as that caused by mitomycin C, 5-fluorouracil, and sulfur mustard [3941]; and contact lens wear [28, 42, 43]. LSCD secondary to other ocular surface disorders has also been reported; these disorders include extensive microbial infection [28], neurotrophic (neural and ischemic) keratopathy [44], bullous keratopathy [45], and extensive ocular surface tumors [46].

Animal models have been established to investigate the pathogenesis of LSCD. Transcription factor PAX6 (paired box 6) is critical in anterior segment and corneal development. Mutations in PAX6 lead to aniridia and LSCD both in humans and mice [47, 48]. In the Pax6+/− mouse model with heterozygous Pax6+/Sey-Neu mutant allele on a congenic CBA/Ca genetic background, unstable corneal homeostasis and features of progressive corneal deterioration are observed. This mouse model has been used to investigate the effect of Pax6 genotype and age on corneal epithelial cells and to explore the pathogenesis of LSCD [49]. Another mouse model of LSCD was created by using topical administration of benzalkonium chloride at high concentrations [50]. Sulfur mustard exposure induces severe ocular injury and late-onset LSCD in humans. Both rabbit [51] and mouse [52] models of sulfur mustard gas injury have been created. Information obtained from studies of these animal models will shed light on different mechanisms by which LSCD develops and aid in the development of appropriate treatments for LSCD arising from different causes.

3. Clinical presentations

3.1. Symptoms

Patients suffering from LSCD may present with a wide variety of symptoms related to poor epithelial wound healing and recurrent erosions. Patients often experience chronic conjunctival redness, decreased vision, photophobia, foreign body sensation, tearing, blepharospasm, and recurrent episodes of pain from recurrent epithelial breakdown. The pain, photophobia, and discomfort are often debilitating. However, most of these symptoms are nonspecific and inadequate to make the diagnosis correctly.

3.2. Clinical findings under slit-lamp biomicroscopy

Slit-lamp biomicroscopy has been the most commonly used method to make the diagnosis of LSCD. Examination under white light without fluorescein staining provides very limited information to make a correct diagnosis of LSCD. Examination under cobalt blue light using fluorescein staining is essential to detect the subtle signs of LSCD, particularly in the mild or early stage of LSCD (Figs. 1F and 1G).

Figure 1.

Figure 1

Clinical presentations and confocal images of LSCD at different stages.

Figure 1A, 1F, 1K and 1P are slit lamp photos under white light. Figure 1B, 1G, 1L and 1Q are slit lamp photos under cobalt blue light. Figure 1C, 1H, 1M and 1R are confocal images of wing cells at central cornea. Figure 1D, 1I, 1N and 1S are confocal images of epithelial basal cells at central cornea. Figure 1E, 1J, 1O and 1T are confocal images of limbal epithelium and the palisades of Vogt.

Healthy corneal epithelium is transparent (A) without fluorescein staining (B). Normal wing cells (C) have a dark cytoplasm, well-defined bright borders, and no visible nuclei. Basal epithelial cells (D), which are characterized with dark cytoplasm, fairly defined cell borders and no visible nuclei, are clearly seen in normal eyes. The subbasal nerve plexus is also visible in this layer. In healthy eyes, the palisades of Vogt may be detected at the limbal area and appear as hyper-reflective, double-contour, linear structures (E). Late fluorescein staining can be detected at mild stage of LSCD, with a clear line of demarcation may be visible between the corneal and conjunctival epithelial cells in sectoral LSCD (F and G). Corneal epithelial cells in mild LSCD have less distinct borders and prominent nuclei (H and I). The density of subbasal nerve plexus decrease dramatically, along with the infiltration of dendritic cells (I). The structure of palisades of Vogt is altered even in mild LSCD. A large number of inflammatory and dendritic cells are sometimes present with blood vessels (J). Epithelial opacity (K) and persistent late fluorescein staining in a vortex pattern (L) are the signs of a more advanced stage. The morphological abnormalities of wing cells (M) and basal cells (N) at central cornea continue to progress. The degradation of subbusal nerve plexus deteriorates further (N). The palisades of Vogt are absent at limbal area, along with enlargement of limbal epithelial cells (O). Epithelial and stromal opacity, neovascularization (P), and persistent epithelial defect (Q) on the corneal surface may be present in severe or total LSCD. Metaplastic epithelial cells are visible in eyes with severe LSCD (R and S). The structures of limbus and palisades of Vogt are totally damaged (T).

LSCD may be progressive or stationary, diffuse or sectoral (partial). Clinical manifestations of LSCD vary depending on the severity and extent of limbal involvement.

3.2.1. Mild stage

In the mild stage of LSCD, slit-lamp examination findings include dull/irregular corneal surface with loss of light reflex, corneal epithelial opacity and loss of limbal palisades of Vogt.

3.2.1.1. Epithelial opacity

Compared with transparent corneal epithelium seen in normal cornea (Figs. 1A and 1B), a dull and irregular reflex of the epithelium that varies in thickness and transparency [53] is usually seen on the affected corneal surface. These abnormal epithelial cells may be a mixture of metaplastic corneal epithelial cells and conjunctival epithelial cells, or only conjunctival epithelial cells without neovascularization [54, 55]. The irregular opacified epithelium can be identified under white light with careful examination, but is better visualized using fluorescein staining under cobalt blue light.

3.2.1.2. Epithelial staining

Fluorescein allows visualization of the abnormal cells and their pattern of distribution under cobalt blue light. Fluorescein tends to pool on the affected area because the abnormal conjunctival/metaplastic epithelium layer tends to be thinner and lacks cell-cell tight junctions [56]. The late staining on the abnormal area remains after 10 minutes and can be visualized even after rinsing with balanced salt solution or eye wash. In the mild or early stage of LSCD, stippled fluorescein staining may be present [43, 53]. As the disease becomes more severe, a clear line of demarcation may sometimes, but not always, be visible between the area covered by the corneal and conjunctival epithelial cells in sectoral LSCD (Figs. 1F and 1G).

3.2.1.3. Loss of palisades of Vogt

The palisades of Vogt are more commonly seen in the superior and inferior limbus. In the early or mild stage of LSCD, there may be flattening at the limbus in the region of palisades of Vogt or loss of palisades of Vogt. However, this presentation is not reliably found in all cases. Loss of palisades is also age-related [57].

3.2.2. Moderate stage

A common feature of moderate stage LSCD is fluorescein staining and epithelial thinning in a vortex pattern. Superficial neovascularization and peripheral pannus may be present. The extent of LSCD can range from sectoral to total involvement of the limbus. If the central visual axis is involved, the vision can be significantly compromised.

3.2.2.1. Vortex keratopathy

In the moderate or intermediate stage of LSCD, the abnormal epithelium forms a confluent sheet spreading in a spiral pattern from the limbus onto the cornea and invading the visual axis occasionally (Fig. 1K). With fluorescein staining, a whorl or vortex pattern of abnormal epithelium can be visualized (Fig. 1L). Sometimes it is referred to as whorl-like epitheliopathy [42, 53, 55], which is typically present in the moderate stage. The abnormal epithelium is prone to erosion, and mild anterior stromal haze may be present in the area affected by LSCD.

3.2.2.2. Superficial vascularization and peripheral pannus

Limbal stem cells and corneal epithelium play a significant role in maintaining the angiogenic balance in favor of avascularity [58]. Conjunctival epithelial cells, which migrate onto the cornea in LSCD, have been shown to attract new vessels and they may lack the ability to secrete certain anti-angiogenic factors found in normal corneal epithelium [56]. Therefore, superficial vascularization of the cornea and peripheral pannus are usually seen in the moderate stage of LSCD [54, 59].

3. 2.3. Severe stage

Recurrent or persistent corneal epithelial defect, corneal neovascularization, corneal stroma scarring and opacification are often present in severe-to-total LSCD.

3.2.3.1. Recurrent/persistent epithelial defects

As the population of functional LSCs declines further, recurrent or persistent epithelial defects often occur (Fig. 1Q). Chronic non-healing corneal epithelial defect or repeated epithelium breakdown followed by slow healing are common clinical manifestations of LSCD. Because of the compromised corneal epithelial barrier, the risk of microbial infection increases [60, 61]. Corneal melt or even perforation that may or may not be related to infection can occur in severe LSCD as a result of the persistent epithelial defect.

3.2.3.2. Stromal neovascularization

Both persistent epithelial defects and deteriorated barrier function of the limbus might contribute to an ingrowth of fibrovascular pannus and neovascularization at deep stroma. Although stromal neovascularization is common in severe LSCD, it can also be found in other eye disorders without LSCD, such as herpetic keratitis and previous microbial keratitis.

3.2.3.3. Stromal scarring and opacity

Severe-to-total deficiency of functional LSCs results in almost complete absence of normal corneal epithelium. Corneal stromal haze often worsens as a result of the longstanding instability of the epithelial surface (Fig. 1P). Stromal scarring, calcification, and eventual opacification often occur at the end stage of LSCD. The severe corneal epithelial and stromal opacity leads to functional blindness. Sometimes total or partial keratinization of the covering epithelium may be present due to associated aqueous tear deficiency rather than LSCD.

4. Current diagnostic methods

4.1. Clinical examination

As noted in Section 3, clinical findings under slit-lamp biomicroscopy have been the main basis for the diagnosis and classification of LSCD. A detailed description of the clinical presentations presently used to diagnose and classify mild, moderate, and severe/total LSCD is provided above and summarized in Table 1.

Table 1.

Grading of LSCD based on the findings of slit lamp biomicroscopy

Report Method Normal (Grade 0) Mild (Grade 1) Moderate (Grade 2) Severe/total (Grade 3)
Deng 53 WL Smooth epithelial surface Dull corneal reflex; Superficial punctate keratitis. Thinning of epithelial surface. Persistent epithelial defect (or history of corneal epithelial defect), with or without vascularization of cornea.
FLS No staining Stippling or late FLS. Persistent, late FLS in a vortex pattern. Same vortex pattern of FLS and pooling at area with epithelial abnormality.
Sacchetti54 WL No epithelial alterations; No superficial neovascularization; No stromal scarring. Epithelial haze in less than half of the sector; Superficial neovascularization involved in less than half of the sectorå Stromal scarring involved in less than half of the sector. Epithelial haze and/or epithelial defect in more than half of the sectorå Superficial neovascularization involved in more than half of the sectorå Stromal scarring involved in more than half of the sector. Epithelial opacity in the entire sectorå Superficial neovascularization involved in the entire Stromal sectorå scarring involved in the entire sector.
Dua55 WL Loss of limbal anatomy; Irregular, thin epithelium without notable vascularization. Irregular, thin epithelium; Filaments and erosions; Fibrovascular pannus. Superficial and deep vascularization; Persistent epithelial defects; Fibrovascular pannus; Scarring, keratinization, and calcification.
FLS Stippled FLS of area covered by abnormal epithelium. Whorls or wedges pattern FLS with the broad base toward the limbus and the narrow curving apex toward corneal center.
* Chan42 WL Epithelial opacity arising from limbus; Corneal pannus. Corneal neovascularization; Recurrent/persistent epithelial defects; Stromal scarring.
* Djalialian43 FLS Punctate FLS following curve-like path. Linear pattern or a confluent sheet FLS spreading centrally from limbus. At least 6 clock hours of whorl-like FLS.
Short59 WL Cornea clear; Iris detail visible; Pupil is clearly seen. Mild haze of epithelium; Slight loss of iris; Pupil can be seen but is blurred. Moderate haze of epithelium; No iris details visible; Pupil can just be seen with difficulty. Severe haze of epithelium; No iris details visible; Pupil cannot be seen.
*

Study focuses only on LSCD caused by contact lenses.

WL = White light. FLS = Fluorescein staining.

Although fluorescein staining is essential in the diagnosis of LSCD, the technique to instill fluorescein into the conjunctival sac is crucial to elicit the clinical signs of LSCD. Briefly, a sterile fluorescein strip is wetted by approximately 20 μL sterile saline solution and instilled into the inferior conjunctival sac without touching the cornea or limbus. Patients are then instructed to blink several times to allow the fluorescein to spread over the entire ocular surface. Too much fluorescein will mask the pathology and too little fluorescein will not stain epithelium sufficiently, thus compromising its diagnostic effect.

4.2. Impression cytology

Impression cytology is a well-established method to diagnose ocular surface diseases [62, 63]. It is performed by applying a cellulose acetate filter paper, nitrocellulose membrane or Biopore membrane to the ocular surface to remove the very superficial layers of ocular surface epithelium. The specimens can be subjected to hematoxylin and eosin (H&E), Periodic acid Schiff, Papanicolaou, or Giemsa staining to evaluate the morphology of cells taken from ocular surface, both goblet cells and epithelial cells. Impression cytology has been used as a standard technique to study squamous metaplasia and goblet cell loss in ocular surface diseases, such as dry eye disease, chemical injuries, cicatrizing conjunctivitis, vitamin A deficiency, and effects of diverse medications [62].

The presence of goblet cells on the cornea indicates the invasion of conjunctival cells, which is a hallmark of LSCD [45, 6365]. The identification of goblet cells on the specimens taken from corneal surface by impression cytology has been considered as the “gold standard” in the currently used diagnosis system for LSCD. Moreover, impression cytology on the cornea has been used from the first limbal transplantation to evaluate the restoration of corneal phenotype after surgery [66].

The sensitivity of impression cytology in the diagnosis of LSCD is affected by many factors. Filter pore size affects the consistency of cell collection, and the surfactant treatment of the filter paper can reduce cell pick-up. Therefore, surfactant- free filter paper with pore size 0.22–0.44 μm is recommended [63]. Compared to Biopore membrane, cellulose acetate filter paper is easily wetted during the sample acquisition, either from the anesthetic used beforehand and/or reflex tearing, leading to a poorer cell acquisition and staining outcome. The pressure being applied to the membrane also has a direct effect on the number of cells harvested. The more epithelial cells that are sampled, the greater the number of goblet cells detected on the filter/membrane [67]. The location of sampling is also important, especially in sectoral LSCD. Some researchers divide the membrane into two “D” shaped halves or one large round disk (13 mm) to cover the entire corneal and limbal surface, while others use four fan-shaped pieces to achieve better contact between the membrane and ocular surface [63]. Identification of the location where abnormal epithelium is detected is important, especially in cases of sectoral LSCD.

4.3. Limitations of current diagnostic methods

A recent review reported that more than 60% of the interventional studies on LSCD performed from 2003 to 2013 used diagnostic criteria solely based on clinical presentations, and 30% used the combination of clinical signs and impression cytology for the diagnosis of LSCD [68]. Less than 10% of the interventional studies used the combination of slit-lamp biomicroscopy and other diagnostic tests, such as imaging (e.g., in vivo confocal microscopy [IVCM]) and/or molecular tests to detect conjunctival epithelial cells on the cornea.

Several findings indicate that current diagnostic criteria cannot accurately assess the severity of LSCD [6971]. Structural changes occur before the onset of clinical signs. Although clinical signs and presentations are important in the diagnosis of LSCD, many signs described above are not pathognomonic of LSCD and can be found in other ocular disorders. In addition, detection of those subtle clinical signs is operator-dependent and interpretation of clinical signs is subjective. Normal variations of the limbal structure can further complicate the interpretation. For instance, the palisades of Vogt signify a normal healthy limbus. Disarrayed or absence of palisades of Vogt may be an early anatomical change in mild LSCD or as a result of normal variant or aging process [72]. Subtle fluorescein staining patterns in sectoral or partial LSCD may be missed by clinical examination. Some signs present in LSCD, such as fine stippling fluorescein staining and corneal neovascularization, are nonspecific and are present in other conditions that do not have a component of LSCD. Previous studies also suggest that changes in the microstructure precede the clinical presentation in LSCD [53, 69]. Therefore, clinical presentations are insufficient to provide quantitative evaluation for grading the severity of LSCD.

Although the presence of goblet cells on the corneal surface signifies conjunctival epithelial invasion, the absence of goblet cells on the cornea does not necessarily exclude the presence of LSCD. In Stevens-Johnson syndrome, advanced mucous membrane pemphigoid and severe chemical burn, the number of goblet cells is decreased on the ocular surface [7375]. Sensitivity of impression cytology is low in patients with such disorders and may lead to a false-negative result. The sensitivity of impression cytology varies due to a lack of standardization in the collection and cytological staining protocol [76, 77]. Sampling bias exists because of tight junction of corneal epithelial cells. Therefore, impression cytology is not a quantitative test and cannot be used to quantify the severity of LSCD.

It is difficult to distinguish severe LSCD from total LSCD based on the current diagnostic methods. Although the clinical presentations of severe LSCD and total LSCD are similar, their clinical outcome and prognosis after treatment are likely to be different. A recent study found that residual normal limbal epithelial cells were present in eyes that are categorized as total LSCD using the current diagnostic criteria [71]. It has also been observed that the host LSCs reconstructed the corneal epithelium after allogeneic LSC transplantation in a significant number of successful cases [78, 79], suggesting that the residual LSCs in these recipient eyes might be responsible for reconstruction of the normal corneal epithelial surface. The amount of residual functional LSCs likely plays an important role in the clinical outcome of LSCT. Therefore, it is important to distinguish severe LSCD from total LSCD. An accurate diagnosis and staging of LSCD is of paramount importance in the assessment of the efficacy of different treatments for LSCD.

5. Recent advances in the diagnosis of LSCD

To overcome the shortcomings of current diagnostic methods of LSCD, researchers have investigated the utility of newly developed ocular anterior-segment imaging modalities in the diagnosis of LSCD. Moreover, significant progress has been made in identifying molecular markers that can be developed into new diagnostic tests.

5.1. In vivo imaging

5.1.1. In vivo laser scanning confocal microscopy

In vivo laser scanning confocal microscopy (IVCM) is a noninvasive method to visualize the microstructures within the cornea, limbus, and conjunctiva at the single cell level [31, 57, 8084]. IVCM has a lateral resolution of 1 μm, and axial resolution of 4 μm [85]. The morphology of the corneal epithelium, corneal subbasal nerve plexus, corneal stroma, and limbal structure can be visualized in healthy and abnormal eyes. Hence, the use of IVCM in ophthalmology, especially in the studies of LSCD, has expanded greatly over the last decade [18, 27, 53, 6971, 80, 8689].

5.1.1.1 Techniques of IVCM on patients with LSCD

Currently, two types of in vivo confocal microscopes are commercially available: ConfoScan 4 (NIDEK CO., LTD, Japan) and Heidelberg Retina Tomography (HRT) with a cornea module (Heidelberg Engineering GmbH, Germany). With the application of laser as light source, HRT can obtain images of limbus with better quality, thus becoming the main tool in the study of limbus under normal and pathological conditions. Since the typical size of HRT IVCM image is 0.4 x 0.4 mm2, representing only 0.15% of the total corneal area, careful slit lamp examination before IVCM can help to determine the location of pathology, which is important to obtain images with good diagnostic values, especially in patients with sectoral LSCD. Patient cooperation is also crucial for obtaining good confocal pictures. Detailed explanation to patients before the examination will alleviate their anxiety and fear caused by the direct contact from the confocal lens. To look for residual LSCs in cases of severe-to-total LSCD, a quick scan of the entire 360 degrees of limbus using the “Sequence” mode is preferred. Patients is instructed to rotate their eyes by following the target light to position the limbus to the imaging areas.

5.1.1.2. IVCM parameters for the diagnosis of LSCD

The detection of goblet cells on the cornea by IVCM has been reported as a diagnostic marker of LSCD [64, 88]. However, detection of goblet cells by IVCM is examiner-dependent and has the same limitations as those in impression cytology for the diagnosis of LSCD, as discussed previously [27, 64]. Moreover, there is no consensus in regard to the morphologic features of goblet cells observed on the confocal images. Some studies have reported that goblet cells exhibit a hyper-reflective cytoplasm [77, 90], whereas other studies have suggested that the cytoplasm is hypo-reflective [91]. This variation might be due to the secretion status of the goblet cells. Therefore, new parameters to identify abnormal corneal epithelium, limbal epithelial cells, and conjunctival epithelial cells are more precise and are needed to improve the sensitivity and accuracy of IVCM in the diagnosis of LSCD.

5.1.1.2.1. Corneal and limbal epithelium

Normal corneal epithelium is composed of superficial cells, wing cells, and basal cells. Superficial cells usually appear to be loosely arranged, large hexagonal or polygonal flat cells with hyper-reflective cytoplasm. Wing cells, located between superficial cells and basal cells, have a dark cytoplasm, well-defined bright cell borders, and no visible nuclei (Fig. 1C). Basal epithelial cells are the smallest of the three types of corneal epithelial cells, with a diameter of 8–10 μm [85]. They usually appear as a regular mosaic of dark cell bodies with cell borders visible but less clearly defined than those seen in wing cells. The nuclei of basal epithelial cells are often not visible or very faint. The subbasal nerve plexus is visible at the basal and subbasal layer of corneal epithelial cells in normal eyes (Fig. 1D).

Significant microstructural changes in the corneal epithelium are detected in patients with LSCD, even in those with mild- or early-stage LSCD. The epithelial cells in patients with LSCD have less distinct borders and prominent nuclei (Fig. 1H and 1I). In more advanced stages of LSCD, more morphologic abnormalities of the epithelial cells can be seen (Fig. 1M and 1N). In eyes with severe LSCD, epithelial cells are metaplastic (Fig. 1R and 1S) and neovascularization may be seen. Compared with healthy eyes, eyes with LSCD have significantly decreased basal epithelial cell density (31.0% reduction in LSCD eyes) and larger size of basal epithelial cells (19.7% augmentation in LSCD eyes) [53, 69]. There is a positive correlation between the reduction of basal cell density and the severity of LSCD [69]. Although the morphologic changes of the corneal epithelium are not specific to LSCD, the large-scale reduction of basal cell density has been reported only for LSCD. In addition to the changes in morphology and basal cell density, the epithelial thickness at the central cornea is also decreased significantly in patients with LSCD (20.2% reduction in LSCD eyes) [70]. The extent of epithelial thinning is also positively correlated with the degree of LSCD.

The limbal epithelium undergoes morphologic changes similar to those of the corneal epithelial cells, including decreased cellular density (23.6% reduction in LSCD eyes), enlarged cellular size (13.5% augmentation in LSCD eyes), and alterations in the cell morphology [69, 92]. The epithelial thickness at the limbal area is also significantly reduced (38.5% reduction in LSCD eyes) [70]. Similar to the changes found in the central cornea, the reduction of basal limbal epithelial density and limbal epithelium thickness positively correlates with the severity of LSCD. More importantly, there are significant decreases in basal cell density and epithelial thickness in the limbal regions that do not exhibit clinical signs of LSCD in eyes with sectoral LSCD. This finding suggests that these microstructural changes precede the clinical presentation of LSCD.

5.1.1..2.2 Subbasal nerve plexus

In addition to epithelial cells, the subbasal nerve plexus is affected in LSCD. Innervation of the corneal epithelium plays a pivotal role in the maintenance of health and function of the epithelium. Corneal nerves not only protect the ocular surface through an elaborate mechanism of sensation and blink reflex, but also release various trophic factors that regulate epithelial integrity, proliferation, and wound healing [93, 94].

In patients with LSCD, drastic changes in the morphology and the density of the subbasal nerve plexus are observed, which correlate with the severity of LSCD (Fig. 1I, 1N, 1S). Compared with control subjects, subbasal nerve density was reduced by 34.9% in the early stage, 54.0% in the intermediate stage, and 73.5% in the late stage of LSCD [95]. Other distinct morphologic changes of the subbasal nerve plexus in patients with LSCD include severe nerve dropout, short nerve branches, and sharp turns of the branched nerve [95]. In patients with severe LSCD, such as in mucous membrane pemphigoid [96], chronic Stevens-Johnson syndrome, and toxic epidermal necrolysis [31], the subbasal nerve plexus is usually not detectable.

It should be noted that reduced corneal subbasal nerve density can be found under many pathological conditions, including systemic diseases [97, 98], ocular disorders [99101], and alterations caused by ocular surgeries or therapies [102105]. Therefore, this parameter is not specific to LSCD. The sensitivity and specificity of subbasal nerve density in the diagnosis of LSCD requires further investigation.

5.1.1.2.3. Corneal and limbal stroma

The morphology of corneal and limbal stroma is affected in LSCD. A large number of inflammatory and dendritic cells are sometimes present with blood vessels within the epithelial layer and deep stromal layers [33, 106]. The slightly hyper-reflective stroma in normal eyes is replaced by homogenously bright fibrotic structures in LSCD even at mild stage [107].

At the limbal area, the palisades of Vogt may be detected in normal eyes by IVCM. The palisades of Vogt usually appear as hyper-reflective, double-contour, linear structures (Fig. 1E). These structures alternate with islands of epithelial cells that correspond to the inter-palisade rete pegs [80, 82]. In moderately pigmented healthy subjects, a bright fringe of hyper-reflective basal epithelial cells around stromal papilla is usually seen [57]. Limbal projections, which are round cords of stroma that extend into the limbal epithelium, is another structure observed in normal eyes [80, 108].

In patients with LSCD, the structure of the palisades of Vogt is altered or totally absent (Fig. 1J, 1O, 1T) [86, 88, 89]. Limbal projections are often absent. Interestingly, well-demarcated, lacunae-like structures are occasionally found in certain limbal regions of eyes with sectoral LSCD. These structures extend into the deep underlying limbal stroma and contain clusters of highly packed normal limbal epithelial-like cells. These individual structures can extend more than 100 μm into the stroma [109]. The deep location may protect LSCs from surface insults. The exact mechanism by which these lacunae-like structures are formed in sectoral LSCD is unknown.

5.1.1.2.4 Summary

Although the feasibility of using IVCM in the diagnosis of LSCD has been investigated by many researchers [53, 69, 70, 72, 81, 88, 89], the threshold values for the diagnosis in clinical practice cannot be given based on the present studies, because the number of study populations is not large enough. Moreover, basal cell density and epithelial thickness change with age [57, 110]. Therefore, a large study to determine the normal variation due to aging will be necessary to distinguish the degree of change in these two parameters that signify and grade the degree of LSCD. The percentage of reduction or augmentation from the normal level would be a better index than the actual measurement because there might be the variations in the cell counting among different centers or examiners.

5.1.2. Anterior segment optical coherence tomography

5.1.2.1. Principles

OCT, a noninvasive and noncontact imaging technique that was first introduced in 1994 [111], is based on low-coherence interferometry. It compares the time-delay and intensity of infrared light reflected from the tissue structures against a reference beam. To obtain an OCT image, multiple scans are performed to create a series of axial scans (A-scans), and these A-scans are combined into a composite two-dimensional image, or cross-sectional tomography [112].

Anterior segment OCT (AS-OCT) can image ocular anterior segment structures, including the cornea, limbus, anterior chamber and angle without the use of topical anesthesia. Commercially available AS-OCT systems (Table 2) are developed on two different platforms: time-domain and spectral domain (also called Fourier-domain). In time-domain OCT, cross-sectional images are produced by varying the position of the reference mirror [112]. Spectral domain OCT uses a stationary reference mirror [112]. The signal of interference between the sample and reference reflection is detected by varying the wavelength of the light source with time [113]. Time domain OCT has a wider scan range but relatively lower resolution. With the development of spectral OCT, which provides an axial resolution of 3 μm [114], the limbal structure can be better elucidated in normal and pathological conditions [110, 115, 116].

Table 2.

Commercially available AS-OCT instrumentations at present

commercially available AS-OCT manufacturer platform wave length A scans per second resolution scan width
Visante OCT Carl Zeiss Meditec, Germany time-domain 1310nm 2000 15–20μm 16mm
slit-lamp OCT Heidelberg Engineering GmbH, Germany time-domain 1310nm 4000 10–15μm 15mm max
Casia SS-1000 Tomey, Japan swept-source 1310nm 30000 10μm 16mm
RTVue Optovue, USA Fourier-domain 840nm 26000 5μm 2–8mm
regular: 6–9mm
Cirrus OCT Carl Zeiss Meditec, Germany Fourier-domain 840nm 27000 5μm max: 15.5mm
Spectralis Heidelberg Engineering GmbH, Germany Fourier-domain
Fourier-domain
870nm 40000 3.9μm 6mm
Copernicus HR Optopol Technology, Poland high resolution 840nm 52000 3μm 5mm
5.1.2.2. Ocular surface structure
5.1.2.2.1. Corneal and limbal epithelium

AS-OCT provides two methods to measure epithelium thickness. The thickness of the epithelial cell layer within a 6-mm diameter of the central cornea can be automatically measured (Fig. 2A, 2E). The thickness of epithelium, either central cornea or limbal area, can also be manually measured on cross-sectional images (Fig. 2B, 2F) [110]. AS-OCT can measure the epithelial thickness in normal eyes [110, 117] and in eyes with various ocular surface disorders [118, 119]. In LSCD, the thickness of limbal epithelium measured by AS-OCT decreases significantly (Fig. 2D–2F) [87], which is consistent with the findings of confocal imaging [70]. The limbal epithelial thinning correlates with the damage to the palisades of Vogt in LSCD. However, the application of AS-OCT to study the alterations of corneal epithelial thickness in LSCD has not yet been fully developed [70]. The irregularity of the epithelium, the reflectivity alterations of the epithelial layer, and interference caused by hyper-reflective fibrovascular tissue underneath the epithelium in LSCD are all factors that reduce the discrimination of the epithelial layer from the underlying subepithelial scar or anterior stromal haze on the OCT images. These limitations could be resolved by a newer generation of OCT with an ultra-higher resolution.

Figure 2.

Figure 2

AS-OCT images of normal subjects and patients with LSCD

Fig 2A–2C are taken from normal eyes. The thickness of epithelial cell layer within a 6-mm diameter of the central cornea can be automatically measured under pachymetry scan mode (A). The thickness of limbal epithelium can be manually measured on cross-sectional image (B). The palisades of Vogt are visible at limbus in normal eyes (C). Fig 2D–2F are taken from eyes with sectoral LSCD. The area with reduced epithelial thickness found in AS-OCT (E) is corresponding to the affected area shown by slit-lamp examination (D). The cross sectional images of limbus show that the thickness of limbal epithelium decreased at the affected area (F).

5.1.2.2.2. The palisades of Vogt

In vivo visualization of the palisades of Vogt provides information about any structural changes of the limbus in healthy and pathological conditions. Spectral domain OCT has been reported to visualize the palisades of Vogt (Fig. 2C) [120]. This technique may enhance targeted limbal biopsies for transplantation [121]. However, as mentioned above, the palisades of Vogt may be absent in eyes with normal LSC function. IVCM is a more sensitive tool to locate normal limbal epithelium for biopsy.

5.1.2.3. OCT angiography (OCTA)

Recent technological developments have increased the imaging capabilities of OCT in the evaluation of vascular flow; use of OCT for this purpose is called OCT angiography (OCTA). This method visualizes vessels via motion contrast imaging of erythrocyte movement across sequential B-scans [122]. In a preliminary study, OCTA acquired images of the cornea and limbal vasculature with substantial consistency [123]. Although the utilization of OCTA in the diagnosis of LSCD is probably limited because corneal neovascularization is not a specific sign of LSCD, it might be a promising tool to quantify the extent of corneal neovascularization.

5.1.3. Three-dimensional imaging of cornea and limbus

With recent improvement of hardware and software in quantitative corneal imaging by IVCM, three-dimensional images of the cornea and limbus can be obtained. These images allow measurement of corneal sublayer thicknesses, stromal cell and extracellular matrix backscatter, depth-dependent changes in corneal keratocyte density, and the structure of the subbasal nerve plexus [124]. The development of image acquisition, reconstruction, and analysis techniques should further expand the applications of IVCM in the diagnosis of LSCD.

With the progress of volumetric OCT and computer-assisted three-dimensional reconstruction technology, spectral domain OCT has recently been shown to have the capability of visualizing the three-dimensional structure of the limbal area by using reconstructed volumetric images that include the palisades of Vogt, blood vessels, lymphoid channels, Schlemm's canal, trabecular meshwork and corneal nerve fiber bundles [125128]. The palisades of Vogt appear to be connected in the stromal layer as deep as 120 μm below the epithelial surface. These deep invasions of epithelium form an elaborate network [82]. This finding suggests that LSCs are likely to be located very deep in the limbal stroma.

Three-dimensional images of the limbus could provide important structural information on the LSC niche in both normal and pathologic conditions. The structural information could be used as a new parameter to detect residual LSCs and quantify the severity of LSCD.

5.2. Molecular methods

Direct histological staining (e.g., H&E staining and Papanicolaou staining) of impression cytology specimens is used to evaluate the morphology of the epithelium. However, histology cannot distinguish conjunctival epithelial cells from corneal epithelial cells without cell-specific markers.

5.2.1. Molecular biomarkers

Cytokeratins are a group of water-insoluble proteins that form intermediate filaments in epithelial cells and are expressed in distinct patterns during epithelial development and differentiation. Immunocytochemical analysis of impression cytology specimens can identify the specific type of cytokeratin present and hence determine the type of epithelium. Cytokeratin profiles have been used as markers of epithelial origins and as a supplementary diagnostic tool in some studies of LSCD [129, 130].

Table 3 summarizes the biomarkers that have been used in the diagnosis of LSCD and methods to detect them. Cytokeratin 3 and 12 are regarded as specific markers of differentiated corneal epithelial cells, whereas cytokeratin 19 and mucin 1(MUC1) are reportedly conjunctiva-specific markers [54, 81, 131]. However, other reports have shown that cytokeratin 19 is expressed in the limbal and peripheral corneal epithelium and MUC 1 is expressed throughout the entire ocular surface in the normal human eye [132, 133]. Keratin 3 is also expressed in the conjunctiva [81]. Therefore, cytokeratin 3 is less specific than cytokeratin 12 to identify corneal epithelium, and keratin 19 and mucin 1 are not specific for conjunctival epithelium. In contrast, cytokeratin 7 and cytokeratin 13 are expressed in the conjunctival epithelium but not in the corneal epithelium [134, 135]; thus, these two proteins are more specific markers of conjunctival epithelial cells than cytokeratin 19 and MUC1. Cytokeratin 15 has also been reported as the specific marker of conjunctival epithelial cells [136]. However, its application in impression cytology is under investigation. Mucin 5AC is a specific marker of goblet cells [132] which has been used to detect goblet cells.

Table 3.

Biomarkers previously or currently used in the diagnosis of LSCD

biomarker location specificity detection
CK3 [54, 131] cornea + immunohistochemical staining
CK12 [81, 132] cornea +++ immunohistochemical staining
CK19 [54, 131] conjunctiva + immunohistochemical staining
CK15 [136] conjunctiva ++ immunohistochemical staining
CK7 [132] conjunctiva +++ immunohistochemical staining
CK13 [132, 134, 135] conjunctiva +++ immunohistochemical staining
MUC5AC [132, 140] goblet cells +++ immunohistochemical staining
counterstained with hematoxylin
RT-PCR
MUC1 [81] conjunctiva (membrane-associated mucins) + immunohistochemical staining and immunoelectron microscopy

5.2.2. Methods to detect the molecular biomarkers

The traditional technique to detect cytokeratin is immunohistochemistry. Flow cytometric analysis of cells collected by impression cytology is a new approach [137]. Other molecular analytical methods, such as RT-PCR to detect the transcript of Mucin 5AC, are more sensitive and objective than the immunohistochemistry staining [132, 138140].

In recent years, the protein/cytokine analysis of tears has been performed in patients with congenital aniridia using high-performance liquid chromatography, two-dimensional electrophoresis, and liquid chromatography-tandem mass spectrometry [141, 142]. Tear acquisition is easier to perform and less traumatic to the epithelium than impression cytology. The diagnostic value of these proteins/cytokines in tear still needs further investigation and validation because aniridia accounts for only a very small portion of LSCD.

The use of specific markers of conjunctival and corneal epithelia and the application of new techniques to detect these markers increases the specificity and sensitivity of traditional impression cytology. Optimization of these molecular techniques is needed to verify their utility as diagnostic tests. The diagnosis of LSCD will be more reliable using the combination of molecular diagnostic tests and in vivo imaging.

6. Future Developments

The characterization of the cellular structure of the cornea and limbus in healthy eyes using in vivo imaging has increased our understanding of pathophysiology of external eye diseases [85, 110, 143, 144]. Cellular changes in the cornea and limbus occur in LSCD and have been quantified by both IVCM and AS-OCT. Basal cell density, subbasal nerve density and epithelial thickness are three quantifiable measures that have been shown to correlate with the severity of LSCD. With further improvement in image resolution and image analysis software, these quantifiable parameters could be further optimized to establish a threshold level that indicates the occurrence of LSCD and to classify the severity of LSCD.

The three-dimensional structure of the limbus can be mapped by IVCM and AS-OCT, and the number of any residual limbal epithelial cells located in deep limbal crypts/lacunae could be identified. In addition, these residual LSCs could be collected by targeted biopsy for autologous LSC therapy in severe LSCD.

A precise diagnostic test using molecular markers to detect conjunctival epithelial cells on the cornea will be important in confirming the diagnosis of LSCD. A combination of the molecular diagnostic tests, the grading of clinical signs, and the detection of microstructural changes could lead to a precise staging of LSCD and assessment of the clinical outcome of LSC therapy in the future.

The treatment approaches for different severity levels of LSCD are different. In partial LSCD, elimination of ongoing insults to the existing LSCs and optimization of the health of the ocular surface should be the first steps in recovering the LSC population from the remaining LSC pool and restoring LSC function. These initial steps would not require LSC transplantation. It is likely that only in severe or total LSCD, transplantation of LSCs will be necessary. The degree of LSCD that requires LSC transplantation remains to be established. The same criteria to stage LSCD would be used to assess the clinical outcome of different therapies and make future improvement of these therapies possible.

7. Summary

The current diagnostic methods and criteria for LSCD are based solely on clinical examination and traditional impression cytology. They are not accurate and do not permit the quantification of LSC function or LSCD. There is a lack of consensus in the current diagnostic approaches for LSCD. In vivo imaging devices are powerful tools to analyze and quantify the in vivo dynamic changes of the ocular surface structure at the cellular level. A set of standardized criteria can be established by using a combination of grading of clinical presentation, quantitative analysis of ocular surface structural changes, and detection of molecular biomarkers to accurately diagnose and quantify LSCD.

Acknowledgments

This study was supported by an unrestricted grant from Research to Prevent Blindness. SXD received grant support from National Eye Institute (5P30EY000331 and R01EY021797), California Institute for Regenerative Medicine (TR2-01768, CLIN1-08686).

Footnotes

The authors have no commercial or proprietary interest in any concept or product described in this article.

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