FIG 6 .

Immunization with O-Ag⁻ STmGMMA offers protection against infection in the spleen and liver. CD1 mice were immunized with STmGMMA O-Ag⁺ or O-Ag⁻ at time zero and day 14 and subsequently challenged i.p. with 2 × 10⁴ CFU of S. Typhimurium SL1344 at day 28. Bacteria in the spleen (left) and liver (right) were enumerated 3 days after infection. Each circle represents one mouse, and graphs are representative of two independent experiments. One-way ANOVA with Tukey’s multiple-comparison test were used to determine significant differences between groups linked by bars. NI, nonimmunized;*, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001.