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. Author manuscript; available in PMC: 2018 Mar 10.
Published in final edited form as: Nature. 2016 Nov 16;539(7630):583–587. doi: 10.1038/nature20562

Figure 1. The formation of an EcMutS–EcMutL complex alters the diffusion properties of EcMutS.

Figure 1

a, Representative kymographs and illustration showing an EcMutL loaded by an EcMutS sliding clamp. Blue line indicates the mismatch position. b, The distribution of dwell times for the EcMutS–EcMutL complex (τon•EcMutS–EcMutL; mean ± s.e.m.). c, Box plots of D for the EcMutS–EcMutL complex at different NaCl concentrations (n = number of molecules; Methods). d, The distribution of the starting positions for EcMutS (top) or EcMutS–EcMutL complexes on DNA (bottom). There are two possible orientations of the mismatched DNA with mismatch position (blue star; middle panel). Diamonds in top and bottom panels represent individual starting events. Gaussian fit to the top panel distributions is shown as black lines with the mean ± s.d.