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. Author manuscript; available in PMC: 2019 Mar 27.
Published in final edited form as: Vaccine. 2018 Feb 26;36(14):1846–1852. doi: 10.1016/j.vaccine.2018.02.073

Figure 1. Splenocyte cytokine production from plant-DIII immunized mice.

Figure 1

Spleen cells from mice injected with PBS (A) or 25 μg plant-DIII (B–D) were stimulated in vitro with DIII for 24 to 48 hr. The production of IL-4 (A and B), IL-6 (A and C), and IFN-γ (A and D) was quantitated by a multiplex mouse cytokine kit. Mean concentration (pg/ml) and standard deviation (SD) from at least two independent experiments with technical triplicates for each sample are presented. Compared with unstimulated samples, significant induction of cytokines by antigen stimulation are observed (p = 0.002 for IL-4 and IL6 both 24hr and 48 hr; and p =0.002 for IFN-γ 48 hr). Significant difference in cytokine levels are also observed between splenocytes from plant-DIII vaccinated mice and control mice receiving PBS (p = 0.002 for IL-4 and IL-6 both 24hr and 48 hr; and p < 0.002 for IFN-γ 48 hr).