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. 2018 Mar 13;7(3):24. doi: 10.1038/s41389-018-0029-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — 786-O and ACHN cells were grown in serum-supplied medium (SSM) and serum-free medium (SFM) for 5 days, respectively. a Representative images of tumorspheres. Bar, 100 μm. b The protein expression of renal CSCs markers (CD133, CD44, ALDHA1, Oct4, and Nanog) was measured by Western blotting. c The mRNA expression level of renal CSCs was measured by qRT-PCR. d The number of CD133⁺ cells in 786-O and ACHN cells was detected by flow cytometry. Data are expressed as mean ± standard deviation (SD). *p < 0.05, **p < 0.01 compared with SSM group