TABLE 2 .
α-Diversitya
| DNA reaction concn (ng/μl) |
No. of observed OTUs, mean difference (P value)b |
Shannon index, mean difference (P value)c |
Inverse Simpson index, mean difference (P value)d |
|---|---|---|---|
| 2 × 10−1 | −0.664 (0.96) | 0.0138 (0.88) | 0.409 (0.78) |
| 4 × 10−2 | −32.1 (0.06) | −0.070 (0.43) | −1.15 (0.41) |
| 8 × 10−3 | −66.1 (<0.001) | −0.167 (0.07) | −3.22 (0.02) |
| 1.6 × 10−3 | −131 (<0.001) | −0.292 (0.002) | −4.11 (0.004) |
| 3.2 × 10−4 | −194 (<0.001) | −0.450 (<0.001) | −5.74 (<0.001) |
| 6.4 × 10−5 | −233 (<0.001) | −0.630 (<0.001) | −6.23 (<0.001) |
| 1.28 × 10−5 | −262 (<0.001) | −0.926 (<0.001) | −11.8 (<0.001) |
a
Comparison of each dilution with 1 ng/μl gDNA. Abbreviations: gDNA, genomic DNA; OTU, operational taxonomic units.
b
The average number of observed OTUs was 362.
c
The average Shannon index value was 4.14.
d
The average inverse Simpson index value was 26.2.