Table 1. Mating procedure results for the six proof-of-principle AGOC sublines from the F3 to the F7 generation.
Bold entries mark progeny that were used in the subsequent cross. F6-S, F7-O and F7-C are control crosses. No significant differences between the arithmetic means and the theoretical Mendelian ratios were found. See Source Data 1 for raw scores ordered by transgenic sublines.
Table 1—source data 1. Raw scores for all mating procedure result tables ordered by transgenic sublines.
elife-31677-table1-data1.xlsx (36.6KB, xlsx)
DOI: 10.7554/eLife.31677.015
| Gen | Cross | Subline | Progeny | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
 |
 |
 |
 |
 |
 |
 |
 |
Total | |||
| F3 |  |
Theoretical | - | 50.0% | - | - | - | - | - | 50.0% | - |
| AGOC #1 | - | 47.9% (46) | - | - | - | - | - | 52.1% (50) | 96 | ||
| AGOC #2 | - | 52.4% (44) | - | - | - | - | - | 47.6% (40) | 84 | ||
| AGOC #3 | - | 55.1% (49) | - | - | - | - | - | 44.9% (40) | 89 | ||
| AGOC #4 | - | 44.1% (41) | - | - | - | - | - | 55.9% (52) | 93 | ||
| AGOC #5 | - | 47.6% (39) | - | - | - | - | - | 52.4% (43) | 82 | ||
| AGOC #6 | - | 48.2% (27) | - | - | - | - | - | 51.8% (29) | 56 | ||
| Mean | - | 49.2 ± 3.9% | - | - | - | - | - | 50.8 ± 3.9% | 83.3 | ||
| F4 |  |
Theoretical | 25.0% | 25.0% | 12.5% | 12.5% | 12.5% | 12.5% | - | - | - |
| AGOC #1 | 25.6% (30) | 34.2% (40) | 4.3% (5) | 10.2% (12) | 6.0% (7) | 19.7% (23) | - | - | 117 | ||
| AGOC #2 | 27.3% (36) | 28.9% (38) | 15.1% (20) | 8.3% (11) | 9.8% (13) | 10.6% (14) | - | - | 132 | ||
| AGOC #3 | 25.8% (33) | 32.8% (42) | 13.3% (17) | 10.1% (13) | 5.5% (7) | 12.5% (16) | - | - | 128 | ||
| AGOC #4* | 14.0% (14) | 15.0% (15) | 20.0% (20) | 13.0% (13) | 22.0% (22) | 9.0% (9) | 3.0% (3) | 4.0% (4) | 100 | ||
| AGOC #5 | 17.1% (20) | 40.2% (47) | 16.2% (19) | 8.5% (10) | 10.3% (12) | 7.7% (9) | - | - | 117 | ||
| AGOC #6 | 33.6% (39) | 33.6% (39) | 8.6% (10) | 6.9% (8) | 6.1% (7) | 11.2% (13) | - | - | 116 | ||
| Mean | 23.9 ± 7.2% | 30.8 ± 8.5% | 12.9 ± 5.6% | 9.5 ± 2.1% | 9.9 ± 6.3% | 11.8 ± 4.2% | 0.5% | 0.7% | 118.3 | ||
| F5 |  |
Theoretical | 25.0% | - | 25.0% | 25.0% | - | - | 25.0% | - | - |
| AGOC #1 | 27.2% (31) | - | 26.3% (30) | 23.7% (27) | - | - | 22.8% (26) | - | 114 | ||
| AGOC #2 | 28.9% (26) | - | 33.3% (30) | 17.8% (16) | - | - | 20.0% (18) | - | 90 | ||
| AGOC #3 | 24.8% (30) | - | 27.3% (33) | 24.8% (30) | - | - | 23.1% (28) | - | 121 | ||
| AGOC #4 | 19.3% (21) | - | 22.9% (25) | 37.6% (41) | - | - | 20.2% (22) | - | 109 | ||
| AGOC #5 | 28.2% (31) | - | 29.1% (32) | 12.7% (14) | - | - | 30.0% (33) | - | 110 | ||
| AGOC #6 | 26.2% (22) | - | 29.7% (25) | 16.7% (14) | - | - | 27.4% (23) | - | 84 | ||
| Mean | 25.8 ± 3.5% | - | 28.1 ± 3.5% | 22.2 ± 8.8% | - | - | 23.9 ± 4.0% | - | 104.7 | ||
| F6-S |  |
Theoretical | - | - | 50.0% | 50.0% | - | - | - | - | |
| AGOC #1 | - | - | 46.4% (39) | 53.6% (44) | - | - | - | - | 84 | ||
| AGOC #2 | - | - | 50.0% (49) | 50.0% (49) | - | - | - | - | 98 | ||
| AGOC #3 | - | - | 54.0% (68) | 46.0% (58) | - | - | - | - | 126 | ||
| AGOC #4 | - | - | 53.8% (50) | 46.2% (43) | - | - | - | - | 93 | ||
| AGOC #5 | - | - | 51.3% (59) | 48.7% (56) | - | - | - | - | 115 | ||
| AGOC #6 | - | - | 57.0% (49) | 43.0% (37) | - | - | - | - | 86 | ||
| Mean | - | - | 52.1 ± 3.7% | 47.9 ± 3.7% | - | - | - | - | 100.3 | ||
| F6 |  |
Theoretical | - | - | 25.0% | 25.0% | - | - | 50.0% | - | - |
| AGOC #1 | - | - | 20.3% (23) | 24.8% (28) | - | - | 54.9% (62) | - | 113 | ||
| AGOC #2 | - | - | 21.5% (23) | 35.5% (38) | - | - | 43.0% (46) | - | 117 | ||
| AGOC #3 | - | - | 22.9% (27) | 22.9% (27) | - | - | 54.2% (64) | - | 118 | ||
| AGOC #4 | - | - | 22.0% (29) | 22.7% (30) | - | - | 55.3% (73) | - | 132 | ||
| AGOC #5 | - | - | 17.5% (18) | 31.1% (32) | - | - | 51.4% (53) | - | 103 | ||
| AGOC #6 | - | - | 19.8% (22) | 24.3% (27) | - | - | 55.9% (62) | - | 111 | ||
| Mean | - | - | 20.7 ± 1.9% | 26.9 ± 5.2% | - | - | 52.4 ± 4.9% | - | 115.7 | ||
| F7-O |  |
Theoretical | - | - | 100% | - | - | - | - | - | - |
| AGOC #1 | - | - | 100% (94) | - | - | - | - | - | 94 | ||
| AGOC #2 | - | - | 100% (50) | - | - | - | - | - | 50 | ||
| AGOC #3 | - | - | 100% (49) | - | - | - | - | - | 49 | ||
| AGOC #4 | - | - | 100% (79) | - | - | - | - | - | 79 | ||
| AGOC #5 | - | - | 100% (79) | - | - | - | - | - | 79 | ||
| AGOC #6 | - | - | 100% (63) | - | - | - | - | - | 63 | ||
| Mean | - | - | 100 ± 0% | - | - | - | - | - | 69.0 | ||
| F7-C |  |
Theoretical | - | - | - | 100% | - | - | - | - | - |
| AGOC #1 | - | - | - | 100% (101) | - | - | - | - | 101 | ||
| AGOC #2 | - | - | - | 100% (105) | - | - | - | - | 105 | ||
| AGOC #3 | - | - | - | 100% (89) | - | - | - | - | 89 | ||
| AGOC #4 | - | - | - | 100% (54) | - | - | - | - | 54 | ||
| AGOC #5 | - | - | - | 100% (74) | - | - | - | - | 74 | ||
| AGOC #6 | - | - | - | 100% (64) | - | - | - | - | 64 | ||
| Mean | - | - | - | 100 ± 0% | - | - | - | - | 81.2 | ||
*In the AGOC #4 subline, incomplete recombination occurred in the F4 (mCe; mO-mC) double hemizygous generation, as we obtained several F5 individuals that still carried both transformation markers (7.0% in total). We continued the mating procedure with the F5 (mO) and (mC) post-recombination hemizygous progeny.