Figure 6.

CYFIP2-RNP Recruitment in Growth Cones on Axon-Axon Contact

(A) Time-lapse sequence showing the transport of CYFIP2-GFP in Cy3-UTP-RNA-granules in RGC axon shaft, highlighted by arrowheads.

(B) Quantification of the CYFIP2-GFP containing RNPs motions in the RGC axon shaft.

(C) Example of CYFIP2-GFP and Cy3-UTP-RNA granules signals in a RGC growth cone (GC).

(D) Assay used to follow Cy3-UTP-RNA granules movements during axon-axon interactions.

(E) Example of a time-lapse sequence showing the recruitment of Cy3-UTP-RNA granules in the peripheral domain of the GC in response to a heterotypic contact.

(F–H) Quantifications of the relative Cy3-UTP-RNA granules distribution for each time point in isolated GCs (F) (n = 3 experiments), after heterotypic interactions (G) (n = 5 experiments) or homotypic interactions (H) (n = 5 experiments). T = 0 correspond to the cell-contact point.

(I–K) Quantification of Cy3-UTP-RNA granules anterograde transport over time in isolated (I) (n = 3 experiments), after heterotypic interactions (J) (n = 4 experiments) or homotypic interactions (K) (n = 4 experiments), normalized to the average anterograde transport for each axon.

Error bars represent SEM. ^∗p < 0.05 (Mann-Whitney test for F–K). Time stamps are in the format of min:s. Scale bars: 3 μm (A) and 5 μm (C and E).