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. Author manuscript; available in PMC: 2018 Jun 20.
Published in final edited form as: Biochemistry. 2017 Jun 7;56(24):3058–3067. doi: 10.1021/acs.biochem.7b00334

Figure 2.

Figure 2

(A) Absorbance spectra of WT, opt-WT, and Cys-less CYP3A4. Arrows point to spectral differences indicative of a partial type II spectral shift in opt-WT and Cys-less CYP3A4. (B) Spectral changes induced by RIT in Cys-less CYP3A4. (C and D) Equilibrium titration of Cys-less CYP3A4 with BEC and MDZ, respectively. Insets are titration plots and quadratic or hyperbolic fittings. The derived Ks values are listed in Table 1.