Figure 7.

Highly proliferative Sox9^Cyt cells that express ARX, INSM1, and variable glucagon are also present in infant and child pancreata. Control islets of infants (A, C, E, and G) and children (B, D, F, and H) stained for islet endocrine, Sox9, Ki67, and α-cell markers. A and B: Islets stained for synaptophysin (Syn) (green) and Sox9 (red). Insets indicate synaptophysin-Sox9^Cyt copositive cells. C and D: Islets stained for Sox9 (green), ARX (red), and glucagon (Gcg) (white). Insets indicate Sox9^Cyt-ARX-glucagon triple-positive cells, with a Sox9^Cyt ARX+ cell that is clearly glucagon negative visible in the inset of D. E and F: Islets stained for glucagon (white), ARX (green), and Ki67 (red). Insets indicate ARX-Ki67 copositive cells that do not express glucagon. G and H: Islets stained for Sox9 (white), ARX (green), and INSM1 (red). Insets indicate Sox9^Cyt ARX+ cells expressing INSM1. Scale bar: 100 μm. mths, months; wks, weeks; yrs, years.