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. 2018 Feb 3;9(17):13407–13422. doi: 10.18632/oncotarget.24388

Figure 6. SELENOK deficiency alters adhesion and migration of melanoma cells.

Figure 6

(A) Migration rates were measured for w.t. SK-MEL-28 cells and SELENOK deficient Clone 7 cells using electrode-created wounds and resistance to measure movement of cells into cleared electrode areas. (B) Adherence to fibronectin and collagen I was measured and Clone 7 cells found to exhibit increased adherence to both types of fibers compared to w.t. controls. Stained cells were measured using absorbance read on an ELISA plate reader at 595 nm. (C) Transwell assays were performed to test w.t. and Clone 7 cells for migration through fibronectin or collagen I. Migration through both matrices was lower for Clone 7 cells compare to w.t. controls as measured by fluorescent staining of cells. Results represent three independent experiments. Means of replicates, N = 3 for (A) and N = 6 for (C–D), were compared using a student’s t-test and expressed as mean + SEM with *p < 0.05.