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. 2018 Feb 12;9(18):14228–14250. doi: 10.18632/oncotarget.24479

Figure 5. IFNβ induces surface expression of TRAIL in NPC cells.

Figure 5

Figure 5

(A) IFNβ induces surface expression of TRAIL in NPC cell lines HONE-1, HONE-1 EBV, CNE-2, HK-1, TW01 and C17-PDX cells, but not in cell line C666-1 and the immortalized nasoepithelial cell line NP69. TRAIL-expression was first noted 24 h after incubation with IFNβ. Cells were incubated with 1,000 U/ml IFNβ and surface expression was analyzed by flow cytometry comparing IFNβ-treated cells (gray area) against non-treated cells (white area). (B) Immunolocalization of TRAIL in NPC cells. Cells were treated for 72 h with IFNβ and stained for TRAIL as described in “Materials and Methods”. Nuclei were counterstained with Hoechst 33258. Confocal microscopy at 400× magnification demonstrates predominant localization of TRAIL on the cell surface.