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. 2018 Mar 1;10(3):106. doi: 10.3390/v10030106

Table 1.

Primer sequences used in this study.

Gene Sequence (5′-3′) Forward Sequence (5′-3′) Reverse Accession No. a
TLR4 CTCTGCCTTCACTACAGAGA CTGAGTCGTCTCCAGAAGAT AB078418
TLR9 GTGGAACTGTTTTGGCATC CACAGCACTCTGAGCTTTGT AB071394
TGF-β CACGTGGAGCTATACCAGAA TCCGGTGACATCAAAGGACA Y00111
TNF-α ATTCAGGGATGTGTGGCCTG CCAGATGTCCCAGGTTGCAT JF831365.1
β-actin CATCACCATCGGCAACGA GCGTAGAGGTCCTTCCTGATGT U07786
PEDV-M GGTTCTATTCCCGTTGATGAGGT AACACAAGAGGCCAAAGTATCCAT AF353511.1

a The sequences of the two primers were checked using NCBI BLAST Software (NCBI, Bethesda, MD, USA), and no significant alignment with any other animal virus gene was found.