Table 1.
Temperature dependence of thermally inducible intra-cellular D-GFP fusion expression from plasmid pcIpR-D-GFP-timm [fluorescence units (±SE) × 10−3].
| Induction Time (min) | Culture up-Shift from 30 °C ± SE a | ||
|---|---|---|---|
| 37 °C | 39 °C | 42 °C | |
| 0 | 0 (0.2) | 0 (4.0) | 3.2 (0.05) |
| 20 | 0 (0.2) | 1.4 (2.6) | 153.8 (0.5) |
| 40 | 0 (0.3) | 6.7 (2.0) | 415.4 (0.4) |
| 60 | 0.8 (0.3) | 23.2 (3.2) | 518.5 (0.5) |
| 90 | 0 (0.3) | 41.3 (0.3) | 624.6 (0.4) |
| 120 | 7.3 (14.9) | 30.0 (2.9) | 721.2 (0.4) |
| 150 | 10.8 (1.3) | 84.3 (3.4) | 808.4 (0.4) |
| 180 | 23.9 (2.6) | 136.9 (10.7) | 1039.5 (0.7) |
D-GFP is a fusion protein generated by fusing the DNA sequence for green fluorescence protein to DNA sequence for λ capsid decoration protein D. a All data represent triplicate determinations averaged. Then the triplicate average for up-shifted 594 cells (to 37, 39, or 42 °C for the indicated times) was subtracted from the triplicate averaged upshifted 594[pcIpR-D-GFP-timm] whole cells. The standard error (SE) values represent the average of SE values for determinations of 594 cells with or without a plasmid.