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. 2018 Mar 14;13(2):152–159. doi: 10.1111/1749-4877.12291

Lineage‐specific evolution of bitter taste receptor genes in the giant and red pandas implies dietary adaptation

Lei SHAN 1, Qi WU 1, Le WANG 1, Lei ZHANG 1, Fuwen WEI 1,2,3,✉
PMCID: PMC5873442  PMID: 29168616

Abstract

Taste 2 receptors (TAS2R) mediate bitterness perception in mammals, thus are called bitter taste receptors. It is believed that these genes evolved in response to species‐specific diets. The giant panda (Ailuropoda melanoleuca) and red panda (Ailurus fulgens styani) in the order Carnivora are specialized herbivores with an almost exclusive bamboo diet (>90% bamboo). Because bamboo is full of bitter tasting compounds, we hypothesized that adaptive evolution has occurred at TAS2R genes in giant and red pandas throughout the course of their dietary shift. Here, we characterized 195 TAS2R genes in 9 Carnivora species and examined selective pressures on these genes. We found that both pandas harbor more putative functional TAS2R genes than other carnivores, and pseudogenized TAS2R genes in the giant panda are different from the red panda. The purifying selection on TAS2R1, TAS2R9 and TAS2R38 in the giant panda, and TAS2R62 in the red panda, has been strengthened throughout the course of adaptation to bamboo diet, while selective constraint on TAS2R4 and TAS2R38 in the red panda is relaxed. Remarkably, a few positively selected sites on TAS2R42 have been specifically detected in the giant panda. These results suggest an adaptive response in both pandas to a dietary shift from carnivory to herbivory, and TAS2R genes evolved independently in the 2 pandas. Our findings provide new insight into the molecular basis of mammalian sensory evolution and the process of adaptation to new ecological niches.

Keywords: bitter taste receptor gene, dietary adaptation, giant panda, red panda

 

Cite this article as:

Shan L, Wu Q, Wang L, Zhang L, Wei FW (2018). Lineage‐specific evolution of bitter taste receptor genes in the giant and red pandas implies dietary adaptation. Integrative Zoology 13, 152–9.

Supporting information

Table S1 The whole‐genome assemblies used in this study

Table S2 Analyses of selective pressure on the branch of the giant panda and/or red panda for 17 TAS2R genes

Supporting Information

Click here for additional data file. (6.7KB, pdf)

Supporting Information

Click here for additional data file. (47KB, xls)

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Associated Data

This section collects any data citations, data availability statements, or supplementary materials included in this article.

Supplementary Materials

Table S1 The whole‐genome assemblies used in this study

Table S2 Analyses of selective pressure on the branch of the giant panda and/or red panda for 17 TAS2R genes

Supporting Information

Click here for additional data file. (6.7KB, pdf)

Supporting Information

Click here for additional data file. (47KB, xls)

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