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. 2018 Feb 6;6(1):9. doi: 10.3390/proteomes6010009

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Schematic illustration of the urine proteomic screening process. (A) The workflow of MS analysis for proteomics approach. Collected urine samples were precipitated and digested by trypsin in solution. Purified peptides were injected into the MS spectrometry. (B) The data acquired by MS instrument were entered into to the database search for protein identification. Label-free semi-quantification was performed using by emPAI value and subjected to Gene Ontology (GO) annotation and KEGG pathway analysis.

Schematic illustration of the urine proteomic screening process. (A) The workflow of MS analysis for proteomics approach. Collected urine samples were precipitated and digested by trypsin in solution. Purified peptides were injected into the MS spectrometry. (B) The data acquired by MS instrument were entered into to the database search for protein identification. Label-free semi-quantification was performed using by emPAI value and subjected to Gene Ontology (GO) annotation and KEGG pathway analysis.