ABSTRACT
Here, we report the draft genome sequence of a clinical isolate of the uropathogenic strain Escherichia coli KL53. A total of 5,083,632 bp was de novo assembled into 170 contigs containing 89 RNAs and 5,034 protein-coding genes. Remarkable is the presence of the tellurite resistance (ter) operon on a plasmid.
GENOME ANNOUNCEMENT
The genus Escherichia belongs to the family of Enterobacteriaceae, and due to the cooccurrence of antibiotic and heavy metal resistance genes, these organisms can, through plasmid carryover, evolve multidrug and multimetal resistance, including tellurium resistance. Tellurite oxyanions are highly toxic for most forms of life, even at micromolar levels. A group of pathogens, including foodborne pathogen Escherichia coli O157:H7, Klebsiella pneumoniae (1), Bacillus subtilis, Staphylococcus aureus, Yersinia pestis (2), Vibrio cholerae, and Shigella spp., possess tellurite resistance genes; E. coli KL53 has this cluster on a plasmid (3).
We report here the draft genome sequence of a uropathogenic isolate of E. coli strain KL53 serotype O18:H14 recovered from the hospital environment of the Department of Urology of University Hospital in Bratislava, Slovakia. The strain was found to grow at a high concentration of tellurite under laboratory conditions. The complete genome was sequenced by next-generation sequencing. The sequencing libraries were prepared using the Tn-based library preparation chemistry Nextera XT (Illumina, Inc., San Diego, CA, USA), according to the manufacturer’s instructions. One nanogram of total DNA was fragmented and amplified. Purified fragments were quantified fluorometrically with a Qubit fluorimeter (Thermo Fisher Scientific, Waltham, MA, USA). The quality was assessed using a high-sensitivity (HS) DNA chip (Agilent Technologies, Waldbronn, Germany). Paired-end sequencing was performed using an Illumina MiSeq platform (Illumina, Inc.). The sequence quality was checked with the bioinformatics tool FastQC. For the de novo assembly, SPAdes version 3.10.1 was used. Gene identification and annotation were provided by the NCBI Prokaryotic Genome Annotation Pipeline (https://www.ncbi.nlm.nih.gov/genome/annotation_prok/), with the best-placed reference set and GeneMarkS+ version 4.2.
The total sequence length was 5,083,632 bp. De novo assembly of the E. coli KL53 genome resulted in a set of 170 contigs with GC content of 50.77%. The total DNA contains 5,418 genes, of which 5,034 genes were protein-coding and 89 were RNA genes (14 rRNA, 66 tRNA, and 9 noncoding RNA [ncRNA]). The presence of 3 plasmid incompatibility group-determining sequences was detected [IncI1, 100% identity; IncHI2, 100% identity; IncFIB(K), 98.93% identity] by PlasmidFinder (https://cge.cbs.dtu.dk/services/PlasmidFinder/). An exact match of IncI1 plasmid genes repl1-ardA-trbA-sogS-pilL with database alleles 1-4-17-1-3 was determined using pMLST (http://pubmlst.org/plasmid/).
The KL53 strain includes multiple heavy metal resistance genes for zinc (znuC, znuB, zupT, and zntB), copper (copA to copD), a cation efflux system for silver and other heavy metals (cusA, cusC, and cusF), arsenic (arsCBR), and a tellurium resistance system (terZABCDEF and terWY1XY2Y3), part of which was determined to be localized on a plasmid by transconjugation (GenBank accession no. AJ888883) (4).
Multidrug resistance genes include multidrug efflux resistance-nodulation-division (RND) transporter MexE, which is involved in resistance to a number of compounds, including lipophilic antibiotics, multidrug and toxic compound extrusion (MATE) family efflux transporter DinF, Bcr/CflA (bicyclomycin resistance protein), and the BlaEC family class C β-lactamase providing multiresistance to β-lactam antibiotics.
Furthermore, genes for the resistance to bacitracin, UV resistance, and resistance to N4 and lambda phages were detected in annotated draft genome sequence of E. coli KL53.
Accession number(s).
This whole-genome shotgun project has been deposited in GenBank under the accession no. NXEL00000000. This version of the project (01) has the accession no. NXEL01000000 and consists of sequences NXEL01000001 to NXEL01000170.
ACKNOWLEDGMENTS
This publication is a result of the implementation of the project REVOGENE (grant ITMS 26240220067), which is supported by the Research & Development Operational Programme funded by the ERDF, and of the project implementation of Comenius University Science Park-2 phase (grant ITMS 2014+313021D075), which is supported by the Research & Innovation Operational Programme funded by the ERDF.
Footnotes
Citation Soltys K, Vavrova S, Budis J, Palkova L, Minarik G, Grones J. 2018. Draft genome sequence of Escherichia coli KL53. Genome Announc 6:e00220-18. https://doi.org/10.1128/genomeA.00220-18.
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