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. 2017 Jun 16;27(8):755–765. doi: 10.1093/glycob/cwx050

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© The Author 2017. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Fig. 5. — HPAEC-PAD product spectra of Y421 (A) and Y439 (B) variants. Products were obtained from reactions containing 0.5 M sucrose and 2 U/mL of enzymatic activity in 50 mM Soerensen buffer pH 6.6. Samples were evaluated at sucrose conversion of around 70%. (1) 1-kestose, (2) blastose, (3) disaccharide, (4) 6-kestose, (5) disaccharide, (6) neokestose, (7) tetrasaccharide Glu-(Fru)₃, (8) disaccharide, (9) tetrasaccharide, (10) 6-nystose, (11) tetrasaccharide, (12) pentasaccharide and (13) hexasaccharide. Signal intensities may vary between measurements, thus, wild-type Bm-LS was measured with every sample batch to allow comparison, except for samples in Figure 5B. This figure is available in black and white in print and in color at Glycobiology online.