Comparative Effects of Curcumin and Tetrahydrocurcumin on Dextran Sulfate Sodium-induced Colitis and Inflammatory Signaling in Mice

Abstract

Background

Curcumin, a yellow ingredient of turmeric (Curcuma longa Linn, Zingiberaceae), has long been used in traditional folk medicine in the management of inflammatory disorders. Although curcumin has been reported to inhibit experimentally-induced colitis and carcinogenesis, the underlying molecular mechanisms remain largely unresolved.

Methods

Murine colitis was induced by dextran sulfate sodium (DSS) which mimics inflammatory bowel disease. Curcumin or tetrahydrocurcumin was given orally (0.1 or 0.25 mmol/kg body weight daily) for 7 days before and together with DSS administration (3% in tap water). Collected colon tissue was used for histologic and biochemical analyses.

Results

Administration of curcumin significantly attenuated the severity of DSS-induced colitis and the activation of NF-κB and STAT3 as well as expression of COX-2 and inducible nitric oxide synthase. In contrast to curcumin, its non-electrophilic analogue, tetrahydrocurcumin has much weaker inhibitory effects.

Conclusions

Intragastric administration of curcumin inhibited the experimentally induced murine colitis, which was associated with inhibition of pro-inflammatory signaling mediated by NF-κB and STAT3.

INTRODUCTION

Chronic inflammation has been implicated in pathogenesis of a variety of human malignancies. For instance, inflammatory bowel diseases (IBDs), such as ulcerative colitis and Crohn’s disease, has been considered to contribute to human colorectal carcinogenesis (CRC).1 Dextran sulfate sodium (DSS)-induced mouse colitis mimics IBD, and has been widely used in evaluating the preventive and therapeutic potential of many anti-inflammatory agents. However, while DSS-induced colitis is necessary, it is not sufficient to induce CRC. Tanaka et al.2 developed a murine model of colitis-associated colon carcinogenesis, in which colonic tumors developed within 20 weeks by a single intraperitoneal injection of a genotoxic carcinogen azoxymethane, followed by 2.0% to 2.5% DSS in drinking water for seven consecutive days.

COX-2 has been known to play a pivotal role in pathogenesis of CRC.3 Thus, the intake of the selective COX-2 inhibitor celecoxib resulted in the reduction of polyps in patients with familial adenomatous polyposis.4 Another enzyme involved in inflammation and oxidative stress is inducible nitric oxide synthase (iNOS). iNOS, which is overexpressed in colon cancer of humans and rodents, catalyzes the oxidative deamination of L-arginine to produce NO.5 Elevated levels of NO generated by iNOS induce inflammation, gene mutation, tissue damage, and malignant transformation.6 iNOS is likely to stimulate COX-2 activities,7 and hence affects colon carcinogenesis.6 Thus, COX-2 and iNOS are potential targets for the chemoprevention of colon cancer.5 NF-κB is a key transcription factor that mediates inflammatory gene expression, as well as immune responses.8 The 5′-promoter region of genes encoding COX-2 and iNOS contains putative NF-κB binding sites. NF-κB is mediated by several external stimuli that increase mitogen-activated protein kinase activity.9

Alleviating chronic inflammation represents an important component of current chemoprevention strategy. Though some non-steroidal anti-inflammatory drugs have protective effects against IBD, there has been an increased risk of heart attack or stroke observed in people intaking selective COX-2 inhibitors for 18 months or more. Thus, attention has been focused on the nutritional manipulation of colitis, and possibly inflammation- associated CRC. Numerous phytochemicals present in our diet have been shown to possess anti-inflammatory activities.10 One of the well-known dietary chemopreventive agents is curcumin, a yellow ingredient of the Indian spice turmeric (Curcuma longa Linn, Zingiberaceae). Curcumin has long been used in traditional folk medicine in the management of inflammatory disorders.11 It has been reported that curcumin is effective in ameliorating chronic gut inflammation in an animal model, and also in a randomized cross-over trial for the treatment of ulcerative colitis.12 Although there have been several reports demonstrating the inhibition of experimentally-induced colitis and carcinogenesis by curcumin,13–21 the underlying molecular mechanisms remain largely unresolved.

MATERIALS AND METHODS

1. Materials

Curcumin was supplied from LKT Laboratories (St. Paul, MN, USA). Tetrahydrocurcumin was prepared by conventional catalytic hydrogenation of curcumin in the presence of palladium on carbon. DSS (molecular weight, 36,000–50,000) was obtained from ICN Biochemicals, Inc. (Aurora, OH, USA) and MP Biomedical Inc. (Solon, OH, USA). Rabbit polyclonal COX-2 antibody was purchased from Cayman Chemical Co. (Ann Arbor, MI, USA), and iNOS antibody from BD Bioscience (Franklin Lakes, NJ, USA). Primary antibody for β-catenin was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-rabbit and anti-mouse horseradish peroxidase-conjugated secondary antibodies were products of Zymed Laboratories (San Francisco, CA, USA). An oligonucleotide probe containing the NF-κB sequence in the mouse COX-2 promoter region was obtained from Bionics (Seoul, Korea). An oligonucleotide probes harboring the STAT3 consensus sequence was obtained from Santa Cruz Biotechnology.

2. Dextran sulfate sodium-induced colitis

Male ICR mice (5 weeks of age) supplied by Central Lab. Animal Inc. (Seoul, Korea) were housed in climate-controlled quarters (24ºC at 50% humidity) with a 12-h light/12-h dark cycle. Curcumin or tetrahydrocurcumin suspended in 0.05% carboxymethyl cellulose was given orally (0.1 or 0.25 mmol/kg body weight daily) for 7 days. Control animals were given vehicle in lieu of curcumin. After additional one week treatment, mice were given 3% DSS in a tap water. Control mice were given a tap water. During the period of DSS treatment, all mice were subjected to following examinations on daily basis: weight measurement, fecal occult blood, and stool consistency. Mice were sacrificed by cervical dislocation. After measuring the length of the colorectal part, it was cut longitudinally and fixed with 10% formalin before embedded in paraffin. Each section (4 μm) was stained with H&E.

3. Western blot analysis

Colon tissues were subjected to homogenization in ice-cold lysis buffer (150 mM NaCl, 0.5% Triton-X 100, 50 mM, Tris-HCl [pH 7.4], 20 mM ethylene glycol tetraacetic acid, 1 mM dithiothreitol [DTT], 1 mM Na₃VO₄ and protease inhibitor cocktail tablet), and kysis for 30 minutes at 0°C followed by centrifugation at 14,000 rpm for 15 minutes. Supernatant was collected, and the total protein concentration was quantified by using the BCA protein assay kit (Pierce, Rockford, IL, USA). Cell lysates (30–50 μg protein) were boiled in SDS sample buffer for 5 minutes before electrophoresis on 8% to 12% SDS-polyacrylamide gel. Separated proteins were transferred to PVDF membrane, and the blots were blocked with 5% fat-free dry milk PBS containing 0.1% Tween-20 (PBST) or 1% BSA in Tris-buffered saline containing 0.1% Tween 20 (TBST) for 1 hour at room temperature. After washing with PBST or TBST buffer, the membranes were incubated for 2 hours at room temperature with 1:4,000 dilutions of primary antibody for actin, for 12 hours at 4°C with 1:1,000 dilutions of primary antibody for COX-2 or for 2 to 3 days at 4°C with 1:700 dilutions of primary antibody for iNOS. Blots were washed three times with PBST or TBST at 10 minutes intervals followed by incubation with 1:5,000 dilution of respective horseradish peroxidase conjugated secondary antibodies (rabbit, goat, or mouse) for 1 hour and again washed in PBST or TBST for three times. The corresponding proteins were visualized using an ECL detection kit obtained from Amersham Pharmacia Biotech (Buckinghamshire, UK).

4. Preparation of nuclear extracts

The collected colon tissues of mice were homogenized in hypotonic buffer A (10 mM HEPES [pH 7.8], 10 mM KCl, 2 mM MgCl₂, 1 mM DTT, 0.1 mM ethylenediaminetetraacetic acid [EDTA], and 0.1 mM phenylmethylsulfonylfluoride [PMSF]). To the homogenate was added 10% Nonidet P-40 (NP-40) solution, and the mixture was then centrifuged for 2 minutes at 14,000 rpm. The precipitated nuclei were washed twice with buffer A plus 40 μL of 10% NP-40, centrifuged, resuspended in 200 μL of buffer C (50 mM HEPES [pH 7.8], 50 mM KCl, 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1 mM PMSF, 20% glycerol) and centrifuged for 5 minutes at 14,000 rpm. The supernatant containing nuclear proteins was collected and stored at −70°C for further use.

5. Electrophoretic mobility shift assay

The electrophoretic mobility shift assay was conducted using a DNA-protein binding assay kit (Gibco BRL, Grand Island, NY, USA). In brief, the NF-κB oligonucleotide probe 5′-GAG GGG ATT CCC TTA-3′ and the STAT3 oligonucleotide probe 5′-GAT CCT TCT GGG AAT TCC TAG ATC-3′ were labeled with [γ-³²P] ATP by T4 polynucleotide kinase and purified on a Nick column (Amersham Pharmacia Biotech, Little Chalfont, UK). The reaction was carried out in 25 μL of the mixture containing 10 mM Tris-HCl (pH 7.5), 100 mM NaCl, 1 mM DTT, 1 mM EDTA, 4% glycerol, and 0.1 mg/mL sonicated salmon sperm DNA], 10 μg of nuclear extracts, and 100,000 cpm. of [γ-³²P]ATP-end labeled oligonucleotide. The reaction mixtures were incubated at room temperature for 50 min. After addition of 2 μL of 0.1% bromophenol blue, the samples were electrophoresised using 6% non-denaturing polyacrylamide gel at 150 V in a cold room for 2 hours. Finally, the gel was dried and exposed to X-ray film.

6. Statistical analysis

Results are expressed as the means ± SD. Data were analyzed by one-way ANOVA and Student’s t-test.

RESULTS

1. Curcumin attenuates dextran sulfate sodium-induced mouse colitis

DSS treatment for one week caused the macroscopic symptoms of colitis, such as a decrease in body weight and colon length, rectal bleeding and diarrhea. Based on the severity of stool consistency and rectal bleeding, DSS-induced pathogenic conditions were scored from 0 to 4.22 The sum was given into a form of the disease activity index. Oral administration of curcumin at 0.25 mmol/kg significantly attenuated the body weight loss (Fig. 1A) and shortening of colon (Fig. 1B), loose stool, diarrhea, and occult rectal blood (Fig. 2A). Exposure to DSS in drinking water for seven days also resulted in marked inflammation of the colon as reflected by thickening of the mucosal wall (Fig. 2B). Histopathological examination of formalin-fixed and paraffin-embeded colon tissues revealed marked destruction of colonic crypts after DSS treatment, with a progressive demolition of the mucosal barrier. Treatment with curcumin ameliorated DSS-induced inflammatory damage of the colon, and restored the architecture of the mucosal layer (Fig. 2B).

Figure 1.

Effect of curcumin on (A) body weight loss and (B) colon length reduction in dextran sulfate sodium (DSS)-treated mice. Animal treatment and other experimental details are described in Materials and Methods section. *P < 0.05 and **P < 0.01.

Figure 2.

Effect of curcumin on pathophysiologic conditions and histologic changes in dextran sulfate sodium (DSS)-induced murine colitis. (A) Disease activity index (DAI) as the sum of stool consistency and rectal bleeding was scored 0 to 3. Data are expressed as means ± SD (n = 5 per group). *P < 0.05 or **P < 0.01 vs. the DSS-treated group. The distal colon was stained by H&E. (B) Microscopic analysis of H&E-stained tissue sections shows that oral administration of curcumin (0.25 mmol/kg) significantly ameliorated damage of colonic mucosa caused by DSS. (a) Control; (b) DSS alone; (c) DSS + curcumin (0.1 mmmol/kg); (d) DSS + curcumin (0.25 mmol/kg).

2. Curcumin inhibits dextran sulfate sodium-induced DNA binding of NF-κB in mouse colon

NF-κB has been identified as a tumor promoter in inflammation-associated cancer.23 The activation of NF-κB partly contributes to the transactivation of various proinflammatory genes (e.g., COX-2 and iNOS). As shown in Figure 3, DSS administration led to enhancement of NF-κB DNA binding, which was significantly attenuated by curcumin treatment.

Figure 3.

Inhibitory effects of curcumin on dextran sulfate sodium (DSS)- induced DNA binding of NF-κB in mouse colon. The nuclear extracts was prepared from colon of each treatment group. Inhibitory effects of curcumin on DSS-induced NF-κB DNA binding were assessed by electrophoretic mobility shift assay as described in Materials and Methods. To ensure the specific NF-κB DNA binding, a competition assay was performed with 100-fold excess unlabeled oligonucleotides. Data are means ± SD (n = 5 per group). ***P < 0.001 vs. the DSS-treated group.

3. Tetrahydrocurcumin suppresses colitis and activation of proinflammatory signaling to a lesser extent than curcumin in the colon of dextran sulfate sodium-treated mice

Curcumin possesses a highly electrophilic α,β-unsaturated carbonyl moiety, which can react with free sulfhydryl groups of many cellular proteins.24 To examine whether this electrophilic moiety is important for the anti-inflammatory properties of curcumin, we compared its effects on the DSS-induced colitis, activation of NF-κB and and expression of proinflammatory enzymes, with those of the non-electrophilic analogue, tetrahydrocurcumin (Fig. 4A). As compared to curcumin, tetrahydrocurcumin exerted much lesser inhibitory effects on colitis (Fig. 4B) and NF-κB DNA binding (Fig. 5A), Another transcription factor involved in inflammation-associated carcinogenesis is STAT3. Curcumin inhibited DSS-induced DNA binding of STAT3 in mouse colon (Fig. 5B). DSS-induced expression of COX-2 and iNOS, two representative pro-inflammatory enzymes known to be regulated by NF-κB and STAT3 was also attenuated by curcumin administration (Fig. 5C).

Figure 4.

(A) The synthesis of tetrahydrocurcumin by catalytic hydrogenation of curcumin. (B) Effects of curcumin and tetrahydrocurcumin on dextran sulfate sodium (DSS)-induced colitis. Curcumin and tetrahydrocurcumin were treated to mice by gastric intubation at a dose of 0.25 mmol/kg each. Histology of H&E-stained mouse colon was visualized under a microscope. (a) Control; (b) DSS alone; (c) DSS + curcumin; (d) DSS + tetrahydrocurcumin.

Figure 5.

Effects of curcumin (CUR) and tetrahydrocurcumin (THC) on activation of pro-inflammatory transcription factors and expression of their target proteins in the colon of dextran sulfate sodium (DSS)-treated mice. (A, B) The nuclear extracts from the colon of mice were prepared and subjected to electrophoretic mobility shift assay to assess DNA binding of NF-κB and STAT3 as described in Materials and Methods. (C) Colon tissues from mice treated with either CUR or THC and challenged with DSS were lysed. Lysates were subjected to immunoblot analysis to detect the expression of COX-2 and inducible nitric oxide synthase (iNOS).

DISCUSSION

Over the past few decades, there has been a remarkable progress in our understanding the pathogenesis, development and progression of cancer. However, we still do not have effective therapies that can selectively kill cancer cells. Like other human disorders, cancer is basically preventible. Recently, attention has focused on naturally occurring substances, especially derived from plants, capable of intervening in multi-stage carcinogenesis.25 A wide variety of dietary phytochemicals present in fruits, vegetables, and spices have been known to exert cancer preventive effects through multiple mechanisms.10,25,26

Curcumin is an ingredient of the Indian spice turmeric (Curcuma longa L., Zingiberaceae)27 responsible for the characteristic yellow color of curry. Curcumin, as a pharmacologically active ingredient of turmeric, has traditionally been used in the management of inflammatory symptoms.28 Cancer chemopreventive effects of curcumin in relation to its anti-inflammatory activity have been evaluated in various experimental models. Curcumin when given orally suppressed dimethylhydrazine- initiated and DSS-promoted murine colon carcinogenesis.29 NF-κB plays crucial roles in the regulation of inflammatory gene transcription, as well as cancer progression.30 Suppression of DSS-promoted expression of COX-2 and iNOS by curcumin is attributable to its blockade of the NF-κB overactivation.

Besides NF-κB, STAT3 has been reported to be highly activated in patients with IBDs, such as ulcerative colitis and Crohn’s disease,31 as well as in DSS-induced colitis in mice.32 STAT3 plays a key role in colonic inflammation and colitis-associated cancer.33,34 During the colitis-associated tumorigenesis, the activation of STAT3 promotes cell proliferation and survival through upregulation of its target genes, including cyclin D1 and bcl-xl.35 In our previous study, curcumin was found to inhibit DNA binding of STAT3 in DSS-stimulated mouse colon.36 While our work was in progress, there have been several papers published demonstrating the protective effects of curcumin on DSS-induced murine colitis. Thus, oral administration of curcumin inhibited the DSS-induced colitis, and repressed the signal transduction mediated by JAK-STAT3,19,21 as well as IKKβ activity.37

Tetrahydrocurcumin, one of the metabolites of curcumin, is structurally distinct from curcumin, in that it lacks the α,β-unsaturated carbonyl group. The biological activities of this nonelectrophilic analog of curcumin have been extensively investigated, but the results are discordant. Some studies have demonstrated that tetrahydrocurcumin exerts more potent effects than does curcumin, while others report that both compounds have a similar potency.38 In our present study, tetrahydrocurcumin exhibited weaker inhibitory effects compared with curcumin on DSS-induced colitis, DNA binding activity of NF-κB and STAT3, and expression of COX-2 and iNOS. It appears that the relative potency of curcumin and tetrahydrocurcumin relies on the mode of their interactions with the target molecules. If the interaction is covalent in nature, curcumin is expected to be superior to tetrahydrocurcumin, as the latter compound is unable to act as a Michael reaction acceptor due to the lack of the electrophilic functionality. Otherwise, the response may vary depending on the dose, route of administration, duration of treatment, intracellular environment, especially redox status, etc.

Taken together, the above findings suggest that oral administration of curcumin provokes anti-inflammatory effects by inhibiting the activation of NF-κB and STAT3 and their target protein expression. Considering a close association between inflammation and cancer and the role of aberrantly overexpressed NF-κB and STAT3 in tumor promotion, the present study provides the molecular basis for the previously reported chemopreventive effects of curcumin on inflammation-associated carcinogenesis.